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Sikkandar S.,Anna University | Murugan K.,King College | Nair A.,Biozone Research Technologies Pvt Ltd | Tilton F.,Biozone Research Technologies Pvt Ltd
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Objective - Halophilic bacteria present in various high salt locations have shown to possess various commercially viable properties. An understanding of the microbial flora present in salt brines will help in the proper exploitation of this resource.Methods - In the current study, bacteria were isolated from salt brines and characterized. Followed by the morphological and biochemical characterization, the strains were subjected to molecular characterization. Genomic DNA was isolated and the 16s rRNA gene was amplified and sequenced. The sequence so obtained was subjected to sequence alignment analysis. The genetic diversity of the isolated strains were also analysed by RAPD PCR and phylogenetic relationship was established by dendrogram construction.Results - The isolated strains were found to be Haloferax volcanii and Halobacterium salinarium. The genetic diversity analyses of H. salinarium and H. volcanii reveal the resistance of the former to genetic variations.Conclusion - This study analysed the impact of environmental stress on the genotypes of H. salinarium and H. volcanii.

Sheela,Loyola College | Kingsley S.J.,Loyola College | Rayappan F.,Biozone Research Technologies Pvt. Ltd. | Tilton F.,Biozone Research Technologies Pvt. Ltd.
Journal of Pure and Applied Microbiology | Year: 2014

Over the last few years, the interest in Aeromonas infection in India has gone beyond the boundaries due to the increase of diseases which is responsible for watery diarrhea. Most reports have described gastroenteritis, septicemia, meningitis, pneumonia, or surgical wound infections, primarily in immune-compromised patients1. Infections caused by resistant microorganisms often fail to respond to the standard treatment, resulting in prolonged illness costing a financial burden to families and greater risk of death. This bacterium produces a large number of extracellular virulence factors that are closely associated with specific diseases. It is known that Aeromonas spp possess different chromosomal β-lactamase genes and most of the antibiotics have been rendered futile against this environmental pathogen thus forcing mankind to device methods for control and treatment against them by locating new drug targets. In this study, a total of 30 stool samples were collected from patients in an around hospitals in south Chennai with diarrhoea. A large number of A. hydrophila colonies were isolated and their antibiotic susceptibility pattern was analyzed. Amp C beta-lactamase is Ambler class C enzymes that confer resistance to extended spectrum cephalosporins and their detection is crucial, since the phenotypic tests are not standardized leading to ambiguity in interpretation of results. PCR technique detects antibiotic resistance to more than one antibiotic resistant genes thus facilitating to identify the pervasiveness of extended spectrum β-lactamase (ESBL) producing genes such as CTX-M, TEM and SHV in this study.

Sheela S.,Loyola College | Kingsley S.J.,Loyola College | Vadivukkarasi B.,Biozone Research Technologies Pvt. Ltd | Tilton F.,Biozone Research Technologies Pvt. Ltd
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Among the MDR efflux pump mechanisms in Aeromonas hydrophila, the Ahe ABC efflux pump lays a major role in the multiresistance mechanism. The AheB protein structure is predicted using MODELLER 9.9 and the final model is refined by energy minimization. The quality of the refined model is assessed using PROCHECK. The interaction between the efflux pump inhibitors 1-(1-naphthylmethyl)-piperazine (NMP) and phenyl-arginine-β-naphthylamide (PAβN) is analysed in silico by GOLD Software. The results of this study will provide an insight into the understanding of the mechanism of Ahe ABC efflux pump and the identification of the novel method to block the mechanism.

Sikkandar S.,Anna University | Murugan K.,King Saud University | Al-Sohaibani S.,King Saud University | Rayappan F.,Biozone Research Technologies Pvt Ltd. | And 2 more authors.
Journal of Pure and Applied Microbiology | Year: 2013

Halophiles are known to be potent sources of commercially exploitable substances. Recent interest in the halophile research has its focus on the medicinal properties of these microorganisms. In the current study, an attempt was made to isolate carotenoids from two strains of halophilic bacteria-Halobacterium salinarium and Haloferox volcanii. Media optimization was carried out and H. salinarium was found to produce the maximum yield of carotenoids among the strains. The isolated carotenoids were tested for their free radical scavenging activity by DPPH assay and the results validated the known antioxidant activity of carotenoids. A further analysis of the cytotoxic properties of the carotenoids against human liver cancer cell lines hinted at their anticancer properties. This work is a contribution to the recent research on alternative natural sources of medicine.

Balachandran C.,Entomology Research Institute | Duraipandiyan V.,Entomology Research Institute | Duraipandiyan V.,King Saud University | Al-Dhabi N.A.,King Saud University | And 4 more authors.
Applied Biochemistry and Microbiology | Year: 2015

Anticancer activity of 9,10-anthraquinone isolated from soil-derived (Doddabetta forest, Nilgiris, Tamil Nadu, India) filamentous bacterium Streptomyces sp. isolate ERINLG-26 was tested to explore the molecular mechanisms of action. The culture liquid ethyl acetate extract showed high cytotoxic activity against tested colorectal adenocarcinoma cell line COLO320. The ethyl acetate extract was fractionated by column chromatography over silica gel and preparative HPLC. The isolated compound, 9,10-anthraquinone, showed prominent cytotoxic activity in vitro against colorectal adenocarcinoma cell line COLO320. It showed 79.7% cytotoxicity at concentration of compound 300 μg/mL with IC50 value of 75 μg/mL. Treatment of the COLO320 cancer cells with isolated 9,10-anthraquinone significantly reduced cell proliferation and increased formation of fragmented DNA and apoptotic bodies. The expression of p53 and caspase-3 was up-regulated by 9,10-anthraquinone in colorectal adenocarcinoma cell line COLO320. © 2015, Pleiades Publishing, Inc.

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