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Dresden, Germany

Weidner J.,Universitatsklinik Munster | Liedtke T.,Science and Application PARTEC GMBH | Nasdala I.,Science and Application PARTEC GMBH | Brabetz W.,Biotype Diagnostic GmbH | And 2 more authors.
BioSpektrum | Year: 2011

A novel laboratory system is developed for high resolution gel analysis and allows a compact electrophoresis and live imaging in one device. Using newly developed fluorochromes, the system presents a highly improved sensitivity, making it ideal in the field of multiplex PCR differential diagnostic. Source


Toma M.I.,Carl Gustav Carus Institute | Wuttig D.,TU Dresden | Kaiser S.,TU Dresden | Herr A.,Biotype Diagnostic GmbH | And 7 more authors.
Genes Chromosomes and Cancer | Year: 2013

PARK2 is an E3 ligase, known to be involved in ubiquitination of several proteins and to play a role in neuronal protection. The gene PARK2 and its potentially co-regulated gene PACRG have been previously found to be deleted in clear-cell renal cell carcinomas (ccRCCs). The aim of our study was to evaluate the mRNA and protein expression of PARK2 and PACRG in a large cohort of ccRCC, and to investigate their association with outcome. The expression of both genes was measured by quantitative PCR in 94 primary ccRCCs and autologous nonmalignant kidney tissues. PACRG and PARK2 protein expression was determined immunohistochemically using tissue microarrays comprising 133 ccRCCs. The mRNA and protein expression of PARK2 and PACRG was significantly downregulated in ccRCCs compared with nonmalignant tissues. Low levels of PARK2 mRNA were associated with high-grade ccRCC and lymph node metastasis. Patients with low PARK2 mRNA levels showed a higher tumor-specific mortality rate and a shorter overall survival (OS) than those with high PARK2 expression. Patients without PACRG mRNA expression in the tumor had a shorter disease-free survival and OS than those with tumors expressing PACRG. In multivariate analyses, neither PARK2 nor PACRG expression were independent prognostic factors. The protein expression of PARK2 and PACRG was significantly downregulated in ccRCCs (82.8, and 96.9%, respectively), but no association with clinical outcome was noticed. © 2012 Wiley Periodicals, Inc. Source


Trademark
Biotype Diagnostic GmbH and Biotype Ag | Date: 2004-08-03

[ Products used in the biological, biochemical and chemical industries, namely, reagents for molecular-biological biochemical and immunological testing and analyses; ] biological [ and immunological ] test kits comprised of diagnostic reagents, oligo nucleotiede, [ antibodies, ] in vitro diagnostics, controlling reagents for molecular biological and immunological testing and analysis. Diagnostic preparations for medical [ and veterinary ] purposes. Molecular biological and immunological test systems comprised of micro-array scanners, DNA and protein chips, multiplex-PCR test devices comprised of PCR cyclers, apparatus for electrophoresis, optical and electronical equipment for analyzing biological and biochemical samples, [ cannulas, sampling units, reagent and sample dispensers, pipettes, sample labeling and sample identification apparatuses ] and the computer [ hardware and ] software associated therewith. [ Printed matter, namely, pamphlets about reagents for molecular biological, biochemical and immunological testing and analyses, about biological and immunological test kits comprised of diagnostic reagents for scientific, medical and veterinary use, about diagnostic preparations for medical and veterinary purposes, and about molecular biological, biochemical and immunological analysis ]. [ Molecular biological, biochemical and immunological analysis ].


Kick A.,TU Dresden | Bonsch M.,TU Dresden | Mertig M.,TU Dresden | Herr A.,Biotype Diagnostic GmbH | And 3 more authors.
Proceedings of IEEE Sensors | Year: 2010

The detection of DNA hybridization in medical diagnostics ought to be rapid, sensitive and specific. A platform technology based on surface plasmon resonance (SPR) is presented. We use TOPAS® chips with integrated optics and combined with microfluidics. Applying a nanoliter dispenser, thiol-modified single-stranded probe DNA (anti-tag) is deposited on the gold surface of the chips to create a DNA microarray. We fabricate chips with sufficiently high probe density, which is a key factor for DNA chips and can be controlled by adding MgCl2 to the immobilization solution. This technology offers the possibility of detecting PCR products comprising a single-stranded tag sequence being complementary to an anti-tag sequence of immobilized probes on the microarray. Consequently, this universal platform can be applied for detection of DNA hybridization based on the tag/anti-tag system. We demonstrate detection of specific hybridization of different 300 base pairs-long PCR products by SPR within less than five minutes. We checked for expected cross hybridizations with seven base pairs-long sequences at different positions in the tag/anti-tag sequence. Depending on the distance to the sensor surface we could observe crosshybridization if the according complementary sequence part is more distant from the surface. The initial binding rates (response units/min) at different PCR product concentrations were determined. Within five minutes a PCR product concentration of 2.6 nM is sufficient for distinct detection without crosshybridizations. ©2010 IEEE. Source


Kick A.,TU Dresden | Bonsch M.,TU Dresden | Katzschner B.,TU Dresden | Voigt J.,TU Dresden | And 8 more authors.
Biosensors and Bioelectronics | Year: 2010

We report on the development of a new platform technology for the detection of genetic variations by means of surface plasmon resonance (SPR) spectroscopy. TOPAS chips with integrated optics were exploited in combination with microfluidics. Within minutes, the detection of hybridization kinetics was achieved simultaneously at all spots of the DNA microarray. A nanoliter dispenser is used to deposit thiol-modified single-stranded probe DNA on the gold surface of the chips. We investigated the influence of different parameters on hybridization using model polymerase chain reaction (PCR) products. These PCR products comprised a single-stranded tag sequence being complementary to an anti-tag sequence of probes immobilized on the gold surface. The signals increased with increasing length of PCR products (60, 100 or 300 base pairs) as well as with their concentration. We investigated hybridizations on DNA microarrays comprising 90 spots of probe DNA with three different sequences. Furthermore, we demonstrate that sequences with possible hairpin structures significantly lower the binding rate, and thus, the SPR signals during hybridization. © 2010 Elsevier B.V. Source

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