Gangwon, South Korea
Gangwon, South Korea

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ee S.-M.,Yonsei University | Do H.J.,Korea University | Shin M.-J.,Korea University | Seong S.-I.,Biotopia Co. | And 5 more authors.
Journal of Microbiology and Biotechnology | Year: 2013

We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of 5 μM. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as 0.5 μM elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphateactivated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK.


Lee E.J.,Haegangbio Co. | Hwang K.Y.,Biotopia Co. | Lee H.-S.,Chonbuk National University | Chung N.,Korea University
Journal of Applied Biological Chemistry | Year: 2011

An isolate of actinomycetes was obtained from farmland soil and designated as Streptomyces sp. A1022 based on cultural characteristics, 16S rDNA, DNA gyrase subunit B (gyrB), and fatty acid analyses. When strain A1022 was grown on chrome azurol S agar plate, it did not produce siderophore. However, strain A1022 had a strong antagonism and broad spectrum against six out of nine tested phytopathogens including Alternaria logipes, Cercospora canescece, Colletotrichum gloeosporioides, Diaporthe citri, Magnaporthe grisea, and Sclerotinia soleroforum. These results suggest that Streptomyces sp. A1022 could be employed as a potential biocontrol agent for the development of biopesticide having broad spectrum.


Lee C.W.,Catholic University of Korea | Seo J.Y.,Catholic University of Korea | Choi J.W.,Catholic University of Korea | Lee J.,Catholic University of Korea | And 5 more authors.
Biochemical and Biophysical Research Communications | Year: 2014

Due to some severe side effects or lack of efficacy of currently used synthetic drugs, such as bisphosphonates (BPs), the search for new therapeutic agents that can more effectively prevent and treat osteoporosis (OP) has been an increasingly important topic of research. In this study, the low-molecular weight hyaluronan (LMW-HA, 50 kDa) produced by enzymatic degradation of high-molecular weight hyaluronan (HMW-HA, 1922 kDa) from Streptococcus zooepidemicus was evaluated in vitro for its antiosteoclastogenic potentials using RAW 264.7 murine macrophage cells. LMW-HA (25-200 lg/ml) dosedependently inhibited the receptor activator of NF-jB ligand (RANKL)-induced tartrate-resistance acid phosphatase (TRAP) activity and the formation of multinucleated osteoclasts. Western blot analysis showed that LMW-HA reduced the RANKL-induced expression of tumor necrosis factor receptor-associated factor 6 (TRAF6), gelsolin and c-Src-proline-rich tyrosine kinase 2 suggesting that it could inhibit actin ring formation of osteoclast cells. In addition, LMW-HA inhibited the bone resorption activity of osteoclastic cells by dose-dependently attenuating the RANKL-induced expression of carbonic anhydrase II and integrin b3. RT-PCR analysis showed that LMW-HA dose-dependently decreased the expression of osteoclast-specific genes, such as matrix metalloproteinase 9 (MMP-9) and cathepsin K, suggesting that it has potential to inhibit the differentiation of osteoclastic cells. Taken collectively, these results suggested that LMW-HA (50 kDa) has significant anti-osteoporotic activity in vitro and may be used as a potent functional ingredient in health beneficial foods or as a therapeutic agent to prevent or treat OP. © 2014 Elsevier Inc.


Kang K.-D.,Biotopia Co. | Kamita S.G.,University of California at Davis | Suzuki K.,Iwate University | Seong S.-I.,Biotopia Co. | Seong S.-I.,University of Suwon
Journal of Invertebrate Pathology | Year: 2011

The progression of baculovirus (BmNPV, BmCysPD, AcMNPV or AcAaIT) infection in larval Bombyx mori and Heliothis virescens (1st, 3rd or 5th instar) was investigated following various starvation regimes. When the larvae were starved for 12 or 24h immediately following inoculation, the median lethal time to death (LT50) was delayed by 9.5-19.2h in comparison to non-starved controls. This corresponded to a delay of 10-23% depending upon the larval stage and virus that was used for inoculation. When a 24h-long starvation period was initiated at 1 or 2days post inoculation (p.i.), a statistically significant difference in LT50 was not found indicating that the early stages of infection are more sensitive to the effects of starvation. Viral titers in the hemolymph of 5th instar B. mori that were starved for 24h immediately following inoculation were 10-fold lower (p<0.01) than that found in non-starved control larvae. Histochemical analyses indicated that virus transmission was reduced in 5th instar B. mori that were starved for 24h immediately following inoculation in comparison to non-starved control larvae. In general, the mass of larvae that were starved immediately after inoculation was 30% lower than that of non-starved control insects. Our findings indicate that starvation of the larval host at the time of baculovirus exposure has a negative effect on the rate baculovirus transmission and pathogenesis. © 2010 Elsevier Inc.


Cai L.,Dankook University | Park Y.S.,Biotopia Co. | Seong S.I.,Biotopia Co. | Yoo S.W.,SeoHa EBF Co. | Kim I.H.,Dankook University
Livestock Science | Year: 2015

The objective of this study was to evaluate the effects of rare earth elements-enriched yeast (RY) on growth performance, nutrient digestibility, meat quality, relative organ weight, and excreta microflora in broiler chickens. A total of 765 ROSS 308 one-day-old broilers with an average initial body weight of 49.0±0.1. g were used in this 4-wk feeding study containing the starter period (d 1 to 14) and the grower period (d 15 to 28). Dietary treatments include: (1) basal diet, free of antibiotics [negative control(NC)], (2) NC+500. mg/kg of RY (RY500), (3) NC+1000. mg/kg of RY (RY1000), (4) NC+1500. mg/kg of RY (RY1500), and (5) antibiotics diet, NC+1000. mg/kg of tiamulin [positive control (PC)]. Broiler chickens were allotted to 5 treatments with 9 replicates (17 broiler chickens/replicate) in a completely randomized design. At the end of the experiment, digestibility of dry matter was increased in broiler chickens fed RY1500 (linear, P=0.011) and PC (P=0.035) diets by 3.7 and 3.9%, respectively, compared with NC diet. The digestibility of gross energy was increased (linear, P=0.019) by 4.4% when broiler chickens were fed RY1500 diet compared with those fed NC diet. Yellowness of breast muscle (linear, P=0.003) was increased by 10.7% in broiler chickens fed RY1500 diet compared with the NC group. However, there was no significant influence on growth performance, relative organ weight, and excreta microflora. In conclusion, the results from this study demonstrated that nutrient digestibility and meat quality were improved slightly in the broiler chickens supplemented with RY. © 2014 Elsevier B.V.


Do H.J.,Korea University | Chung J.H.,Korea University | Hwang J.W.,Korea University | Kim O.Y.,Dong - A University | And 2 more authors.
Food and Chemical Toxicology | Year: 2015

The aim of this study was to determine whether 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI beneficially influences lipid metabolism and mitochondrial function in the liver of mice fed a high-fat diet in addition to the anti-obesity properties of DNJ. Male C57BL/6 mice (n=29; 5 weeks old) were randomly assigned to three groups: normal control diet (CTL, n=10), high-fat diet (HF, n=10), and high-fat diet supplemented with DNJ (DNJ, n=9). After 12 weeks, the HF group exhibited higher overall weight gain, of the liver, and of various fat pads than the CTL and DNJ groups did. The HF group also showed greater expression of C/EBPα and CD36 mRNA in the liver than that in the CTL and/or DNJ groups. In addition, mRNA expressions of AAC and FAS were lower, while mRNA expression of PGC-1β was higher in the liver of the DNJ group than that of the HF group. The hepatic expression of p-AMPK/AMPK was higher in the DNJ group than in the HF group. This study provides novel insight into the protective effect of DNJ supplementation against obesity-induced hepatic lipid abnormalities and mitochondrial dysfunction. © 2014 Elsevier Ltd.


Kim H.S.,University of Suwon | Lee J.Y.,Biotopia Co. | Hwang K.Y.,Biotopia Co. | Cho Y.S.,Biotopia Co. | And 5 more authors.
Korean Journal of Microbiology and Biotechnology | Year: 2011

Thirty Streptomyces sp. and 200 Bacillus sp. isolated from Korean soils and traditional foods were screened for their abilities to inhibit α-glucosidase and produce 1-deoxynojirimycin (DNJ). This screening identified a Bacillus sp. bacterium that strongly inhibited α-glucosidase and produced high levels of DNJ from Chungkookjang, a Korean traditional food. The bacterium was characterized in terms of its biochemical and molecular biological properties such as sugar utilization, cellular quinone composition, cell wall fatty acid composition, and 16S rDNA sequence. In addition, scanning electron microscopy was used to visualize the morphology of the bacterium. These analyses identified the bacterium as B. subtilis, a bacterium with Generally Recognized as Safe (GRAS) status. The selected strain was named B. subtilis MORI.


The present invention relates to a polypeptide associated with the biosynthesis of 1-deoxynojirimycin which is a substance that inhibits -glucosidase activity, to a polynucleotide coding therefor, to a vector comprising the polynucleotide, to a transformant comprising the vector, and to a method in which the transformant is used in order to produce either a polypeptide associated with the synthesis of 1-deoxynojirimycin or 1-deoxynojirimycin. The polynucleotide of the present invention can be used in the volume production of 1-deoxynojirimycin, or can be used in studies searching for novel 1-deoxynojirimycin derivatives using recombinant technology, and hence is useful in developing pathogenic virus inhibitors and hypoglycemic agents for diabetic patients.


PubMed | Biotopia Co. and Catholic University of Korea
Type: Journal Article | Journal: International immunopharmacology | Year: 2015

Panax ginseng C.A. Meyer has been traditionally consumed to prevent or treat various medical disorders due to its diverse health benefits. Polysaccharides isolated from Panax ginseng have been known to possess various pharmacological activities, including immune modulating, anti-diabetic, and anti-obesity properties. Despite the increasing number of reports on the bioactivities of ginseng polysaccharides, little is known regarding the medicinal potential of ginseng-derived oligosaccharides. In this study, we prepared a lower-molecular weight oligosaccharide (GOS, MW. 2.2kDa) from ginseng polysaccharides (MW. 11-605kDa) by enzymatic degradation and evaluated for its immunostimulating activities in RAW 264.7 murine macrophage cells. GOS was shown to be a glucan type oligosaccharide mainly containing glucose residues (97.48 in molar %). Treatment with GOS (100-500g/ml) dose-dependently enhanced the production of TNF-, IL-6, and NO in RAW 264.7 cells. Western blot analysis indicated that GOS dose-dependently induced the phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), p38, and nuclear factor B (NFB), which are upstream signalling molecules for cytokine production. While GOS was not cytotoxic to the RAW 264.7 macrophage cells at the concentration tested (up to 1000g/ml), when B16F10 melanoma cells were co-cultured with the GOS-activated macrophages, the cell viability of melanoma cells was dose-dependently decreased through the induction of apoptotic cell death. Taken together, these results suggested that ginseng marc-derived GOS has anti-cancer activity in vitro against melanoma cells by potentiating macrophage function.


PubMed | Dankook University, University of Manitoba, Biogenic Co., Biotopia Co. and Kansas State University
Type: Journal Article | Journal: Journal of animal physiology and animal nutrition | Year: 2016

The objective of this study was to determine the effects of rare earth element-enriched yeast (RY) on egg production, coefficient of total tract apparent digestibility (CTTAD), egg quality, excreta gas emission and excreta microbiota of laying hens. A total of 216 ISA brown laying hens of 52weeks of age were used in a 5-week feeding trial and data were collected every week. Birds were randomly allotted to three dietary treatments each with six replicates and 12 hens per replicate. Each cage (38cm width50cm length40cm height) contained one hen. Treatments consisted of corn-soya bean meal-based diet supplemented with 0, 500 or 1000mg/kg of RY. From weeks 55 to 56, inclusion of RY linearly increased (p<0.05) egg production. The CTTAD of nitrogen was increased (linear, p<0.05) with increasing dietary level of RY. In week 55, yolk height and Haugh units were increased linearly (p<0.05) with increasing dietary RY content. However, no significant effects were observed in terms of excreta emissions and excreta microbiota in laying hens. In conclusion, dietary supplementation with RY improved egg production and CTTAD of nitrogen and slightly improved egg quality in laying hens of the late period of peak egg production.

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