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Chuncheon, South Korea

Do H.J.,Korea University | Chung J.H.,Korea University | Hwang J.W.,Korea University | Kim O.Y.,Dong - A University | And 2 more authors.
Food and Chemical Toxicology | Year: 2015

The aim of this study was to determine whether 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI beneficially influences lipid metabolism and mitochondrial function in the liver of mice fed a high-fat diet in addition to the anti-obesity properties of DNJ. Male C57BL/6 mice (n=29; 5 weeks old) were randomly assigned to three groups: normal control diet (CTL, n=10), high-fat diet (HF, n=10), and high-fat diet supplemented with DNJ (DNJ, n=9). After 12 weeks, the HF group exhibited higher overall weight gain, of the liver, and of various fat pads than the CTL and DNJ groups did. The HF group also showed greater expression of C/EBPα and CD36 mRNA in the liver than that in the CTL and/or DNJ groups. In addition, mRNA expressions of AAC and FAS were lower, while mRNA expression of PGC-1β was higher in the liver of the DNJ group than that of the HF group. The hepatic expression of p-AMPK/AMPK was higher in the DNJ group than in the HF group. This study provides novel insight into the protective effect of DNJ supplementation against obesity-induced hepatic lipid abnormalities and mitochondrial dysfunction. © 2014 Elsevier Ltd. Source


Lee E.J.,Haegangbio Co. | Hwang K.Y.,Biotopia Co. | Lee H.-S.,Chonbuk National University | Chung N.,Korea University
Journal of Applied Biological Chemistry | Year: 2011

An isolate of actinomycetes was obtained from farmland soil and designated as Streptomyces sp. A1022 based on cultural characteristics, 16S rDNA, DNA gyrase subunit B (gyrB), and fatty acid analyses. When strain A1022 was grown on chrome azurol S agar plate, it did not produce siderophore. However, strain A1022 had a strong antagonism and broad spectrum against six out of nine tested phytopathogens including Alternaria logipes, Cercospora canescece, Colletotrichum gloeosporioides, Diaporthe citri, Magnaporthe grisea, and Sclerotinia soleroforum. These results suggest that Streptomyces sp. A1022 could be employed as a potential biocontrol agent for the development of biopesticide having broad spectrum. Source


Seo J.Y.,Catholic University of Korea | Lee C.W.,Catholic University of Korea | Choi D.J.,Catholic University of Korea | Lee J.,Catholic University of Korea | And 2 more authors.
International Immunopharmacology | Year: 2015

Panax ginseng C.A. Meyer has been traditionally consumed to prevent or treat various medical disorders due to its diverse health benefits. Polysaccharides isolated from Panax ginseng have been known to possess various pharmacological activities, including immune modulating, anti-diabetic, and anti-obesity properties. Despite the increasing number of reports on the bioactivities of ginseng polysaccharides, little is known regarding the medicinal potential of ginseng-derived oligosaccharides. In this study, we prepared a lower-molecular weight oligosaccharide (GOS, MW. 2.2 kDa) from ginseng polysaccharides (MW. 11-605 kDa) by enzymatic degradation and evaluated for its immunostimulating activities in RAW 264.7 murine macrophage cells. GOS was shown to be a glucan type oligosaccharide mainly containing glucose residues (97.48 in molar %). Treatment with GOS (100-500 μg/ml) dose-dependently enhanced the production of TNF-α, IL-6, and NO in RAW 264.7 cells. Western blot analysis indicated that GOS dose-dependently induced the phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), p38, and nuclear factor κB (NFκB), which are upstream signalling molecules for cytokine production. While GOS was not cytotoxic to the RAW 264.7 macrophage cells at the concentration tested (up to 1000 μg/ml), when B16F10 melanoma cells were co-cultured with the GOS-activated macrophages, the cell viability of melanoma cells was dose-dependently decreased through the induction of apoptotic cell death. Taken together, these results suggested that ginseng marc-derived GOS has anti-cancer activity in vitro against melanoma cells by potentiating macrophage function. © 2015 Elsevier B.V. Source


Cai L.,Dankook University | Park Y.S.,Biotopia Co. | Seong S.I.,Biotopia Co. | Yoo S.W.,SeoHa EBF Co. | Kim I.H.,Dankook University
Livestock Science | Year: 2015

The objective of this study was to evaluate the effects of rare earth elements-enriched yeast (RY) on growth performance, nutrient digestibility, meat quality, relative organ weight, and excreta microflora in broiler chickens. A total of 765 ROSS 308 one-day-old broilers with an average initial body weight of 49.0±0.1. g were used in this 4-wk feeding study containing the starter period (d 1 to 14) and the grower period (d 15 to 28). Dietary treatments include: (1) basal diet, free of antibiotics [negative control(NC)], (2) NC+500. mg/kg of RY (RY500), (3) NC+1000. mg/kg of RY (RY1000), (4) NC+1500. mg/kg of RY (RY1500), and (5) antibiotics diet, NC+1000. mg/kg of tiamulin [positive control (PC)]. Broiler chickens were allotted to 5 treatments with 9 replicates (17 broiler chickens/replicate) in a completely randomized design. At the end of the experiment, digestibility of dry matter was increased in broiler chickens fed RY1500 (linear, P=0.011) and PC (P=0.035) diets by 3.7 and 3.9%, respectively, compared with NC diet. The digestibility of gross energy was increased (linear, P=0.019) by 4.4% when broiler chickens were fed RY1500 diet compared with those fed NC diet. Yellowness of breast muscle (linear, P=0.003) was increased by 10.7% in broiler chickens fed RY1500 diet compared with the NC group. However, there was no significant influence on growth performance, relative organ weight, and excreta microflora. In conclusion, the results from this study demonstrated that nutrient digestibility and meat quality were improved slightly in the broiler chickens supplemented with RY. © 2014 Elsevier B.V. Source


ee S.-M.,Yonsei University | Do H.J.,Korea University | Shin M.-J.,Korea University | Seong S.-I.,Biotopia Co. | And 5 more authors.
Journal of Microbiology and Biotechnology | Year: 2013

We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of 5 μM. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as 0.5 μM elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphateactivated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK. Source

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