Winooski, VT, United States
Winooski, VT, United States

Time filter

Source Type

Larson B.,BioTek Instruments Inc. | Moeller T.,tap tap tap | Cameron G.,Bioreclamation IVT | Mailliet J.,BioTek Instruments GmbH
BioSpektrum | Year: 2014

Typically in vitro screening has incorporated primary hepatocytes cultured in a two dimensional (2D) format. However, when cultured and studied in this fashion they rapidly lose their key functions and dedifferentiate over the course of only a few days. Here we present data demonstrating the differences in response between human primary cells cultured in 2D and a new 3D cell culture system which has the benefits of a collagen hydrogel with tissue-like properties. © 2014, Springer-Verlag Berlin Heidelberg.


Larson B.,BioTek Instruments Inc | Moeller T.,tap tap tap | Cameron G.,Bioreclamation IVT | Mailliet J.,BioTek Instruments GmbH
BioSpektrum | Year: 2014

Typically in vitro screening has incorporated primary hepatocytes cultured in a two dimensional (2D) format. However, when cultured and studied in this fashion they rapidly lose their key functions and dedifferentiate over the course of only a few days. Here we present data demonstrating the differences in response between human primary cells cultured in 2D and a new 3D cell culture system which has the benefits of a collagen hydrogel with tissue-like properties. © 2014, Springer-Verlag Berlin Heidelberg.


Sheehan A.J.,TGR BioSciences | Goodrich W.,BioTek Instruments Inc. | Banks P.,BioTek Instruments Inc. | Crouch M.F.,TGR BioSciences | Osmond R.I.W.,TGR BioSciences
Assay and Drug Development Technologies | Year: 2013

We describe a cellular assay for detection of phosphorylation of endogenous proteins, whereby cells are seeded, treated, and assayed for modulation of phosphorylation in a single microplate well. The procedure is coupled to a rapid, one-wash sandwich enzyme-linked immuno-sorbent assay, enabling results to be obtained within 3-4 h from cell seeding. The assay was tested in two separate cellular systems, namely, HeLa and MCF-7 cells. When using the one-well protocol with Akt phosphorylation as a model, the response to a number of agonists was the same as the response obtained using cells treated in a separate microplate, using a conventional lysate transfer approach. The assay procedure was automated, and quantitative pharmacological data on three known inhibitors of the PI3-kinase signaling pathway was obtained within 4 h from seeding cells, with six dispense steps, and a single wash cycle. Thus, the protocol affords a reliable means of assaying for cellular signaling events in different cell types, and is amenable to automation. © 2013 Mary Ann Liebert, Inc.


Larson B.,BioTek Instruments Inc. | Berry D.,Global Cell Solutions | Justice B.,Global Cell Solutions | Gainer T.,Life Technologies
Bio Tech International | Year: 2010

A novel three-dimensional (3D) cell culture method, wherein cells are attached to microcarriers, is compared to traditional 2D culture methods. Results suggest that the 3D method offers similar results compared to the 2D method along with improvements in overall cost, time, in situ behaviour and automation-readiness.


Wang D.,U.S. National Institutes of Health | Qian X.,U.S. National Institutes of Health | Rajaram M.,U.S. National Institutes of Health | Rajaram M.,BioTek Instruments Inc. | And 2 more authors.
Oncotarget | Year: 2016

The RHO family of RAS-related GTPases in tumors may be activated by reduced levels of RHO GTPase accelerating proteins (GAPs). One common mechanism is decreased expression of one or more members of the Deleted in Liver Cancer (DLC) family of Rho-GAPs, which comprises three closely related genes (DLC1, DLC2, and DLC3) that are down-regulated in a wide range of malignancies. Here we have studied their comparative biological activity in cultured cells and used publicly available datasets to examine their mRNA expression patterns in normal and cancer tissues, and to explore their relationship to cancer phenotypes and survival outcomes. In The Cancer Genome Atlas (TCGA) database, DLC1 expression predominated in normal lung, breast, and liver, but not in colorectum. Conversely, reduced DLC1 expression predominated in lung squamous cell carcinoma (LSC), lung adenocarcinoma (LAD), breast cancer, and hepatocellular carcinoma (HCC), but not in colorectal cancer. Reduced DLC1 expression was frequently associated with promoter methylation in LSC and LAD, while DLC1 copy number loss was frequent in HCC. DLC1 expression was higher in TCGA LAD patients who remained cancer-free, while low DLC1 had a poorer prognosis than low DLC2 or low DLC3 in a more completely annotated database. The poorest prognosis was associated with low expression of both DLC1 and DLC2 (P < 0.0001). In cultured cells, the three genes induced a similar reduction of Rho-GTP and cell migration. We conclude that DLC1 is the predominant family member expressed in several normal tissues, and its expression is preferentially reduced in common cancers at these sites.


Patent
Biotek Instruments Inc. | Date: 2015-10-08

Optimized automatic focusing of a microscope objective based on a cross correlation between a representative focus metric scan and a focus metric scan of a sample to be imaged.


Patent
Biotek Instruments Inc. | Date: 2014-10-17

A microplate stacker capable of removing and replacing standard microplate lids by separating a microplate from a lid located directly above the microplate in a stack of microplates and lids.


Patent
Bio Tek Instruments Inc. | Date: 2016-07-11

Non-image based autofocusing in an imaging system including an autofocus light source on a movable carriage variably positioned with offset in parallel to an optical axis of an objective.


PubMed | BioTek Instruments Inc.
Type: Journal Article | Journal: Combinatorial chemistry & high throughput screening | Year: 2011

ADME-Tox testing examines the effects of an organism, tissue or cell on a compound, as well as the effects that the compound has on an organism, tissue or cell, and has gained in importance in the overall drug discovery process over the past twenty years. This is due to the rising percentage of drug candidate attrition in the 1990s and early 2000s due to adverse ADME/Tox profiles. The increased importance placed upon ADME/Tox testing has brought about new types of in-vitro assay technologies utilizing microplates to deliver the most pharmacologically relevant data. These tests, however, have typically been performed sequentially, where multiple assays over multiple microplates are used. This typically leads to increased time and cost required to generate the required information, and can sacrifice data quality. Multiplexed assays, however, where more than one piece of data can be attained from a single well or a single microplate, performed using appropriate liquid handling and detection instrumentation, can improve data quality and reduce the time and expense required to attain this information.


PubMed | BioTek Instruments Inc.
Type: Journal Article | Journal: Journal of laboratory automation | Year: 2012

Cell-based drug absorption assays, such as Caco-2 and MDCK-MDR1, are an essential component of lead compound ADME/Tox testing. The permeability and transport data they provide can determine whether a compound continues in the drug discovery process. Current methods typically incorporate 24-well microplates and are performed manually. Yet the need to generate absorption data earlier in the drug discovery process, on an increasing number of compounds, is driving the use of higher density plates. A simple, more efficient process that incorporates 96-well permeable supports and proper instrumentation in an automated process provides more reproducible data compared to manual methods. Here we demonstrate the ability to perform drug permeability and transport assays using Caco-2 or MDCKII-MDR1 cells. The assay procedure was automated in a 96-well format, including cell seeding, media and buffer exchanges, compound dispense, and sample removal using simple robotic instrumentation. Cell monolayer integrity was confirmed via transepithelial electrical resistance and Lucifer yellow measurements. Proper cell function was validated by analyzing apical-to-basolateral and basolateral-to-apical movement of rhodamine 123, a known P-glycoprotein substrate. Apparent permeability and efflux data demonstrate how the automated procedure provides a less variable method than manual processing, and delivers a more accurate assessment of a compounds absorption characteristics.

Loading BioTek Instruments Inc. collaborators
Loading BioTek Instruments Inc. collaborators