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Fu R.-H.,China Medical University at Taichung | Liu S.-P.,China Medical University at Taichung | Ou C.-W.,China Medical University at Taichung | Huang C.-M.,China Medical University at Taichung | And 2 more authors.
Acta Biomaterialia | Year: 2010

Cell-based assays have become important tools in the pharmaceutical and biotechnology industries. However, observing and monitoring molecules in cells that mimic the physiological environment is often difficult. Dynamic processes not only increase the accuracy of simulations, but also improve our understanding of the function and regulation of molecules within cells. In this study we used chitosan as a multifunctional biomaterial for selective micropatterning of cells, peptide delivery and covalent bonding with quantum dots (QD) to decrease the cytotoxicity of QD. Our results demonstrate the efficacy of chitosan-QD-peptide-Alexa Fluor 488 in controlling the spread and spatial organization of cells. Cationic chitosan also provided an efficient delivery mechanism to live cells. We used the shift from green to red fluorescence of the chitosan dual color QD peptide to detect biological activity. This methodology has potential applications in high throughput screening of inhibitors and activators of biological mechanisms and pathways and for use in the pharmaceutical industry. © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. Source

Fu R.-H.,China Medical University at Taichung | Wang Y.-C.,BioTechPlex Corporation | Liu S.-P.,China Medical University at Taichung | Huang C.-M.,China Medical University at Taichung | And 6 more authors.
Cell Transplantation | Year: 2011

Stem cells are a natural choice for cellular therapy because of their potential to differentiate into a variety of lineages, their capacity for self-renewal in the repair of damaged organs and tissues in vivo, and their ability to generate tissue constructs in vitro. Determining how to efficiently drive stem cell differentiation to a lineage of choice is critical for the success of cellular therapeutics. Many factors are involved in this process, the extracellular microenvironment playing a significant role in controlling cellular behavior. In recent years, researchers have focused on identifying a variety of biomaterials to provide a microenvironment that is conducive to stem cell growth and differentiation and that ultimately mimics the in vivo situation. Appropriate biomaterials support the cellular attachment, proliferation, and lineage-specific differentiation of stem cells. Tissue engineering approaches have been used to incorporate growth factors and morphogenetic factors - factors known to induce lineage commitment of stem cells - into cultures with scaffolding materials, including synthetic and naturally derived biomaterials. This review focuses on various strategies that have been used in stem cell expansion and examines modifications of natural and synthetic materials, as well as various culture conditions, for the maintenance and lineage-specific differentiation of embryonic and adult stem cells. Copyright © 2011 Cognizant Comm. Corp. All rights reserved. Source

Fu R.-H.,China Medical University at Taichung | Hran H.-J.,China Medical University at Taichung | Chu C.-L.,China Medical University at Taichung | Chu C.-L.,National Health Research Institute | And 7 more authors.
Biotechnology Letters | Year: 2011

Modulation of dendritic cell (DC) fate and function may be one approach for the treatment of inflammatory and autoimmune diseases. n-Butylidenephthalide (BP), derived from Angelica sinensis, at 40 μg/ml significantly decreased the secretion of interleukin-6 and tumor necrosis factor-α by lipopolysaccharide (LPS)-stimulated activation of cultured murine DC2. 4 cells (P < 0. 01). LPS-induced major histocompatibility complex class II (P < 0. 05), CD86 (P < 0. 01) and CD40 (P < 0. 01) expression on DC2. 4 cells was also inhibited by BP. The endocytic capacity of LPS-stimulated DC2. 4 cells was increased by BP (P < 0. 01). The antigen-presenting capacity of LPS-stimulated DC2. 4 cells was decreased by BP (P < 0. 05). Moreover, we confirmed BP attenuates the responses of LPS-stimulated activation of DCs via suppression of NF-κB-dependent pathways. © 2011 Springer Science+Business Media B.V. Source

BioTechPlex Corporation | Date: 2004-06-28

Computer software components for a cardiovascular system data collector, processor and display, namely, software for a computer-based system comprising a data acquisition module, an event detector module, a signal sampling module, and an analysis module, for collecting, organizing, and displaying medical data.

BioTechPlex Corporation | Date: 2006-01-31

system comprising membrane holders, fluid chambers, thermostats, and devices for monitoring concentrations, for use in measuring ion flow and water flow through membranes.

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