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Srivastava M.,Mg Post Graduate College | Prabhuji S.K.,Biotechnology and Molecular Biology Center
Medicinal Plants | Year: 2013

The oils of neem (Azadirachta indica), madhuca (Madhuca indica), pongamia (Pongamia glabra), castor (Ricinus communis), eucalyptus (Eucalyptus globulus) and extract of turmeric (Curcuma longa) were used for in vitro protease production and growth of three variants of T. rubrum. The plant extracts were used in 1, 3, 5, 10, 20, 30, 50 μl/ml concentrations in acetone for determining MIC values. Different concentrations of the plant oil / extracts were mixed uniformly in basal synthetic medium and incubated at 30±2 °C and the growth and protease activity was recorded at 24 h interval. The detection of inhibitory excretion of extracellular protease along with retardation of in vitro growth may further strengthen the role of enzyme in the pathogenesis of the organism.

Prabhuji S.K.,Biotechnology and Molecular Biology Center | Rao G.P.,Indian Agricultural Research Institute
Medicinal Plants | Year: 2012

Ayurveda is considered to be the science of life and this makes it an earlier medical science having a positive concept of health to be achieved through a balanced blend of physical, social, moral, mental and spiritual welfare. In Ayurveda, Homoeopathy, Unani, Siddha and several other systems of traditional medication the plants constitute the major resources. The potency of different medicinal plants in controlling diseases has been reviewed together with the status of popular chemically assembled drugs that exhibit several side-effects and after-effects. The present day shift of peoples' inclination towards tribal medicines, ayurvedic, homoeopathic and unani system of medicines is an indication that these constitute the futuristic medications.

Studies on watermoulds-particularly isolation, taxonomic criteria, occurrence and phenology-have been conducted by several aquatic mycologists in different geographical regions of the world. However, the ecology of fungi, particularly watermoulds, has received very little attention. In this contribution, the occurrence, seasonal periodicity, population density and distribution of watermoulds in water bodies and soils in different geographical areas of the globe, are reviewed. Various types of water and soil sampling techniques and the quality of the resulting data are also reviewed. Data from different geographical areas of the globe indicate that distinct eccentric and centric Occurrence Zones of the world may be identified for the members of saprolegniaceae, and an altitude-latitude relationship has also been elucidated. On the basis of the occurrence and phenology of watermoulds in aquatic and terrestrial conditions, the interdependence between aquatic and terrestrial conditions is also discussed. © International Society for Tropical Ecology.

Prabhuji S.K.,Biotechnology and Molecular Biology Center | Sinha S.K.,UP
Journal of Advanced Zoology | Year: 2010

The oomycete members, which have earlier been identified as the basic pathogens for dermatomycoses, have also been found involved in deep mycoses causing damage to deep muscular tissues. The observations and correlations on the dermatomycosis resulting into mortality have been reviewed. The histopathology of dermal tissues in certain fresh-water fishes, viz., Anabas testudineus Bl., Channa punctatus Bl., Chela laubuca Ham. and Colisa lalius Ham., parasitized by oomycetous fungi, have been studied and work reviewed. Dermal Ulcerative Syndrome has also been critically discussed.

Prabhuji S.K.,Biotechnology and Molecular Biology Center | Gaur R.,Awadh University | Tripathi A.,KNIPSS | Srivastava G.K.,Biotechnology and Molecular Biology Center | Srivastava R.,Biotechnology and Molecular Biology Center
Medicinal Plants | Year: 2013

Antifungal activity of Saccharomyces cerevisiae culture, isolated from the toddy of Borassus flabellifer, has been studied in vitro on fish pathogenic watermoulds, viz., Achlya orion, Saprolegnia diclina, Aphanomyces laevis and Allomyces arbuscula. Poison-culture method has been used during the experimental procedure. The results have indicated that the effect of Sachharomyces cerevisiae culture was fungistatic and not fungicidal.

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