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Tahir M.S.,Government College University Lahore | Adnan A.,Government College University Lahore | Syed Q.,Biotechnology and Food Research Center
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

First time a simple, sensitive and unified quantification method has been developed to analyze the complete class of angiotensin II receptor antagonists which are used in the treatment of hypertension either alone or in combination with some other drugs. The most important advantage of developed method was that the eight separate drugs can be determined on a single chromatographic system without modifications in detection wavelength and mobile phase. The drugs were separated on a Purospher Star 4.6 mm × 25 cm, 5 μm, C18 column maintained at 40 °C with 1 mL min-1 flow rate using ultra violet detection at 254 nm. Good separation (Rs > 2.0) was achieved in a short analysis allowing simultaneous determination of all eight sartans. The effect of variation in flow rate, detection wavelength and column oven temperature was also studied. The proposed method was statistically validated in terms of precision, accuracy, linearity, specificity and robustness. The newly developed method proved to be specific, robust and accurate for the quantification of eight sartans in commercial saqlain highnoon@yahoo.com, © 2016 Elsevier B.V. Source

Aftab M.N.,The University of Lahore | Ikram-Ul-Haq,The University of Lahore | Baig S.,Biotechnology and Food Research Center
Pakistan Journal of Botany

The present study is focused on the improvement of Bacillus licheniformis through random mutagenesis to obtain mutant having enhanced production of bacitracin. Many isolates of Bacillus licheniformis were isolated and the isolate GP-40 produced maximum bacitracin production (16±0.72 IU/mL). Treatment of Bacillus licheniformis GP-40 with ultraviolet (UV) radiations increased bacitracin production to 29±0.69 IU/mL. Similarly, treatment of vegetative cells of GP-40 with chemicals like N-methyl N'-nitro N-nitroso guanidine (MNNG) and Nitrous acid (HNO2) increased bacitracin production to 35±1.35 IU/mL and 29±0.89 IU/mL respectively. Studies regarding the combined effect of UV and chemical treatment on parental cells exhibited significantly higher titers of bacitracin with maximum bacitracin production reached to 47.6±0.92 IU/mL. An increase of 2.97 fold production of bacitracin in comparison to wild type was observed. Mutant strain was highly stable and produced consistent yield of bacitracin even after 15 generations. On the basis of kinetic variables, notably μ(h-1)max, Yp/x qp, Qp and Qx mutant strain B. licheniformis UV-MN-HN-8 was found to be a hyperproducer of bacitracin. Source

Saeed A.,Biotechnology and Food Research Center | Iqbal M.,Biotechnology and Food Research Center | Iqbal M.,Bank of The West | Iqbal Sahibzada K.,The University of Lahore | Parvez S.,PCSIR Head Office
Pakistan Journal of Botany

In this study, the adsorption capacity of free and loofa sponge-entrapped microalga Kirchneriella contorta to remove Cr6+ from aqueous solution was investigated. This is the first reported study of biosorption by K. contorta. The effects of the experimental conditions, such as pH, initial Cr6+ concentration, sorbent-sorbate contact time, and quantity of sorbent mass, on Cr6+ removal efficiency were studied. The Cr6+ sorption on K. contorta was found to be highly pH dependent and the maximum uptake capacity was achieved at pH 1.0. The adsorption isotherms study showed the maximum sorption capacity of the loofa sponge-immobilized biomass of K. contorta (LIBKC) of 100.84 mg g-1, which was much higher than 80.61 mg g-1 of the free biomass of K. contorta (FBKC). The adsorption equilibrium data showed a better fit on the monolayer Langmuir adsorption isotherms model with the regression coefficient (r2) greater than 0.99 for both FBKC and LIBKC. The rate of Cr6+ removal followed the pseudo second-order kinetics equation. The LIBKC on treatment with NaOH resulted in 97% Cr6+ recovery and its complete regeneration. The regenerated LIBKC was reused in five repeated cycles without appreciable loss of its metal sorption ability. The potential of LIBKC in a fixed-bed continuous flow column bioreactor for the sorption of Cr6+ from the metal contaminated water was also investigated. Source

Iqbal M.,Biotechnology and Food Research Center | Saeed A.,Biotechnology and Food Research Center
Pakistan Journal of Botany

An indigenous strain of blue green microalga was entrapped within reticulated fibrous network of loofa sponge disc to produce immobilized hybrid disc biosorbent (IHDB) and successfully used for the removal and recovery of Cd2+ from aqueous solution. The two biosorbents used as the symbiotic building block to produce IHDB, were the filamentous blue green alga Phormidium sp. (B1) and loofa sponge discs (B2). Maximum biosorption capacity of B1 and B2 was noted respectively to be 37.06 and 5.32 mg Cd2+ g-1 biosorbent. However, when the two biosorbents were combined to form IHDB, the biosorption capacity (48.53 mg g-1) was increased by 30.95%, 812.21%, respectively as compared to the ability of B1 and B2 when used alone, and by14.51% than the sum of individual abilities of the two biosorbents. The kinetics of Cd2+ removal by IHDB was rapid, with 91.81% of Cd2+ biosorption occurring within first 30 min., and equilibrium was reached after 60 min of contact. The Langmuir and Freundlich adsorption isotherm models were used for mathematical description of the sorption equilibrium. Equilibrium data fitted very well to the Langmuir model in the studied range of concentration (5-200 mg l-1). The biosorbed Cd2+ was desorbed by washing the IHDB with dilute HCl (50 mM) and regenerated IHDB was reused in seven biosorption-desorption cycles without an apparent decrease in metal biosorption capacity. The metal removing capacity of IHDB was also tested in continuous flow fixed-bed column bioreactor and found to be highly effective in removing Cd2+ from aqueous solution. The results suggested that IHDB could be used as low-cost and environment-friendly biosorbent for the efficient removal of Cd2+ from aqueous solution. Source

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