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Ben Jemia M.,Biotechnologic Center Borj Cedria Technopark | Kchouk M.E.,Biotechnologic Center Borj Cedria Technopark | Senatore F.,University of Naples Federico II | Autore G.,University of Salerno | And 3 more authors.
Chemistry Central Journal | Year: 2013

Background: As a part of our investigation on Tunisian medicinal plants, we have carried out a phytochemical investigation of the hexane extracts from leaves of Cistus libanotis, C. villosus and C. monspeliensis, evualuating also their possible antiproliferative activity in vitro.Results: The major compounds of hexane extracts were identified and quantified by GC-MS. The composition of the three species, although belonging to the same genus, is completely different. The antiproliferative activity was evaluated against murine monocyte/macrophages (J774.A1), human melanoma cells (A-375), and human breast cancer cells (MCF-7), showing major activity against the human melanoma cell line A-375.Conclusions: The chemical composition of the hexane extracts from the three Cistus species can be useful in the chemosystematics of this complex genus. The preliminary antiproliferative activity against human melanoma cell line A-375 deserve further investigations in order to determine the compounds, or their combinations, which are the main responsible for the antiproliferative activity and its possible mechanism(s) of action. © 2013 Ben Jemia et al; licensee Chemistry Central Ltd.

Loizzo M.R.,University of Calabria | Ben Jemia M.,Biotechnologic Center Borj Cedria Technopark | Senatore F.,University of Naples Federico II | Bruno M.,University of Palermo | And 2 more authors.
Food and Chemical Toxicology | Year: 2013

The chemical composition of Cistus creticus, Cistus salvifolius, Cistus libanotis, Cistus monspeliensis and Cistus villosus essential oils has been examined by GC and GC-MS analysis. Height-nine constituents were identified in C. salvifolius oil, sixty in C. creticus, fifty-six in C. libanotis, fifty-four in C. villosus, forty-five in C. monspeliensis. Although the five species belong to the same genus, the composition showed interesting differences. Essential oils were screened also for their potential antioxidant effects (by DPPH, ABTS, FRAP and β-carotene bleaching test) and their acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity, useful for prevention and treatment of Alzheimer's disease. C. monspeliensis exhibited the most promising activity in β-carotene bleaching test (IC50 of 54.7μg/mL). In FRAP test C. libanotis showed a value of 19.2μM Fe(II)/g. C. salvifolius showed the highest activity against AChE (IC50 of 58.1μg/mL) while C. libanotis, C. creticus, C. salvifolius demonstrated a good inhibitory activity against BChE with IC50 values of 23.7, 29.1 and 34.2μg/mL, respectively. Overall our results could promote the use of the essential oil of different Cistus species as food additives and for formulation of herbal infusion or nutraceutical products. © 2013 Elsevier Ltd.

Jemia M.B.,Biotechnologic Center Borj Cedria Technopark | Senatore F.,University of Naples Federico II | Bruno M.,University of Palermo | Bancheva S.,Bulgarian Academy of Science
Records of Natural Products | Year: 2015

Volatile components from florets, leaves and stems and branches of Centaurea aeolica Guss. harvested in Lipari, Sicily, Italy, were analysed by gas phase chomatography (GC) and gas chomatography mass spectrometry (GC-MS). The main constituents were β-eudesmol, caryophyllene oxide, (E)-12-norcaryophyll-5-en-2-one and hexahydrofarnesylacetone in flowers, hexahydrofarnesylacetone, 2-methyloctadecane and tricosane in the leaves and hexadecanoic acid, caryophyllene oxide and β-eudesmol in the stems and branches. The analysis of the essential oil of the aerial parts of Centaurea diluta Aiton gave mainly fatty acids and derivatives, the main ones being hexadecanoic acid and (Z,Z)-9,12-octadecadienoic acid methyl ester. © 2015 ACG Publications. All rights reserved.

Jemia M.B.,Biotechnologic Center Borj Cedria Technopark | Tundis R.,University of Calabria | Maggio A.,University of Palermo | Rosselli S.,University of Palermo | And 5 more authors.
Journal of Functional Foods | Year: 2013

A comparative study of phytochemicals content and biological properties of eight Rosmarinus officinalis (rosemary) populations (RO1-RO8) collected in different areas of Tunisia was carried out. Two of the main rosemary constituents, rosmarinic and carnosic acids, were quantified by an NMR technique. Carnosic acid content was higher than that of rosmarinic acid. The non-polar constituents were examined by GC and GC-MS. Total phenols and flavonoids content were also determined in order to discuss the possible correlation between these phytochemicals and bioactivity. Antioxidant activity was investigated through different in vitro assays. Sample RO3 from a sub-humid area showed the highest potency in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay (IC50 of 6.74μg/mL) while RO5 from arid area exhibited the highest radical 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging activity (IC50 of 5.92μg/mL). The most active sample in Ferric Reducing Antioxidant Power (FRAP) assay was RO4 (62.21μM Fe(II)/g), followed by RO1-RO3 and RO8 harvested in sub-humid areas with values in the range 49.61-58.72μM Fe(II)/g. Extracts were able to inhibit acethylcholinesterase (AChE) and butyrylcholinesterase (BChE). Sample RO5 exhibited a promising AChE inhibitory activity (IC50 of 4.47μg/mL). Thus, we suggest rosemary extracts as a functional food ingredient for preventing AD. © 2013.

Jemia M.B.,Biotechnologic Center Borj Cedria Technopark | Wannes W.A.,Biotechnologic Center Borj Cedria Technopark | Ouchikh O.,Biotechnologic Center Borj Cedria Technopark | Bruno M.,University of Palermo | Kchouk M.E.,Biotechnologic Center Borj Cedria Technopark
Natural Product Research | Year: 2013

The present investigation focuses on the methanolic extract obtained from Geranium robertianum L. (Geraniaceae) (Herb Robert), a herbal plant used in traditional medicine for the treatment of human and animal diseases. The antioxidant capacities of the extract were evaluated using 1,1-diphenyl-2- picrylhydrazyl radical, β-carotene/linoleic acid and reducing power and metal chelating activity assays. The amount of total phenolic content, flavonoids and condensed tannins was very high, and the correlation between the antioxidant activity potential and total phenolic level of the extract was pointed out. © 2013 Taylor & Francis.

Wannes W.A.,Biotechnologic Center Borj Cedria Technopark | Marzouk B.,Biotechnologic Center Borj Cedria Technopark
Journal of Food and Drug Analysis | Year: 2016

This study aimed to characterize the chemical composition and antioxidant activity of the oil and the methanolic extract of Myrtus communis var. baetica seed. The oil yield of myrtle seed was 8.90%, with the amount of neutral lipid, especially triacylglycerol, being the highest, followed by phospholipids and glycolipids. Total lipids and all lipid classes were rich in linoleic acid. The content of total phenols, flavonoids, tannins, and proanthocyanidins of the methanolic extract and the oil from myrtle seed was determined using spectrophotometric methods. Antioxidant activities of the oil and the methanolic extract from myrtle seed were evaluated using 1,1-diphenyl-2-picrylhydrazyl radical scavenging, β-carotene-linoleic acid bleaching, and reducing power and metal chelating activity assays. In all tests, the methanolic extract of myrtle seed showed better antioxidant activity than oil. This investigation could suggest the use of myrtle seed in food, industrial, and biomedical applications for its potential metabolites and antioxidant abilities. © 2015.

Kraiem Z.,Biotechnologic Center Borj Cedria Technopark | Aidi Wannes W.,Biotechnologic Center Borj Cedria Technopark | Zairi A.,Biotechnologic Center Borj Cedria Technopark | Ezzili B.,Biotechnologic Center Borj Cedria Technopark
Scientia Horticulturae | Year: 2010

The effects of cutting date and position (apical, basal and central) on rooting ability and fatty acid composition from Carignan (Vitis vinifera L.) shoot were determined. Root number and weight depended of the cutting date and position. Only in the case of the cutting in the basal position, there was a highly positive correlation between number and percentage of roots (r = 0.95) during sampling date. Concerning the influence of the cutting date, the root number by cutting oscillated in a saw tooth. The root weight and percentage showed a positive correlation for the three cutting positions and they increased with time. Cutting date and position had an irregularly effect on the contents of total lipid and different fatty acids. Independently of cutting position, the contents of oleic acid (C18:1) and palmitic acid (C16:0) correlated negatively with those of linoleic (C18:2) and linolenic acids (C18:3) during sampling date. © 2010 Elsevier B.V. All rights reserved.

PubMed | Biotechnologic Center Borj Cedria Technopark
Type: Journal Article | Journal: Natural product communications | Year: 2012

The volatile constituents of the aerial parts of Centaureaformanekii Halacsy and the aerial parts, capitula and roots of C. orphanidea Heldr. & Sart. ex Boiss. ssp. thessala (Hausskn.) Dostl from Greece were extracted by hydrodistillation and analyzed. The main components in C. formanekii were hexadecanoic acid (13.6%), delta-elemene (9.1%), and spathulenol (6.9%). The main components in C. orphanidea ssp. thessala were gamma-elemene (26.1%) and caryophyllene oxide (13.2%) in the aerial parts, hexadecanoic acid (33.5%) and heptacosane (6.3%) in the capitula, and hexadecanoic acid methyl ester (22.0%) and a-chamigrene (14.0%) in the roots. The chemotaxonomic significance with respect to other previously studied species of the same sections (Phalolepis and Achrolopus, respectively) is discussed.

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