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Singh S.,Sunder Deep Pharmacy College | Khan Md.K.,Biotech Park | Sharma S.K.,Sunder Deep Pharmacy College | Singh L.,Sunder Deep Pharmacy College
Journal of Chemical and Pharmaceutical Sciences | Year: 2014

An alarming increase in bacterial strains resistant to existing antimicrobial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to current antimicrobials. Hamelia patens extracts was studied for antibacterial and antifungal activity against various clinical isolates of the bacteria and fungi, in varying concentration by Agar well diffusion method and serial dilutions. The extracts showed pronounced concentration dependent antibacterial activity against Gram positive and Gram negative bacteria and also antifungal activity. Various extracts of Hamelia patens inhibited the growth of Staphylococcus aureus, Bacillus subtilis, Pseudomonas flurescens, Escherichia coliand Aspergillus niger, Penicillium chrysogenum, Alternaria alternata. Six extracts of Hamelia patenswere analyzed for their antioxidant activity by Hydrogen peroxide scavenging activity. The six extract along with the reference sample, Gallic acid were further analysed to determine toal phenolic content by Folin - Ciocalteau method. The data obtained in the in vitro models clearly establish the antioxidant potency of all extracts. © 2014, SPB Pharma Society. All rights reserved. Source


Saxena S.,Babasaheb Bhimrao Ambedkar University | Kesharwani R.K.,Babasaheb Bhimrao Ambedkar University | Singh V.,Babasaheb Bhimrao Ambedkar University | Singh S.,Biotech Park
International Journal of Bioinformatics Research and Applications | Year: 2013

Geminiviruses are single-stranded circular DNA viruses causing leaf curl disease in papaya crop. Post-Transcriptional Gene Silencing (PTGS), also known as RNAi, acts as a natural antiviral defence mechanism and plays a role in genome maintenance and development in plants. PTGS suppression by viruses makes the plant RNA silencing machinery inefficient. Three geminiviral genes namely AV2, AC2 and AC4 are found to play the role in suppression of RNA silencing. siRNA degrades the target mRNA in a homology-dependent manner. In-silico designing of siRNA against these three genes of geminiviruses infecting Carica papaya was done using bioinformatics tools. This strategy may provide PTGS by specifically targeting the viral genes involved in suppression of plant RNA silencing machinery. Copyright © 2013 Inderscience Enterprises Ltd. Source


Gupta S.,Biotech Park | Misra G.,Hygia Institute of Pharmaceutical Education and Research | Pant M.C.,University of Lucknow | Seth P.K.,Biotech Park
Protein and Peptide Letters | Year: 2012

Head and neck squamous cell carcinoma (HNSCC) is a major cause of cancer related death. The epidermal growth factor receptor (EGFR) pathway is over expressed in HNSCC. EGFR regulates the HNSCC by inducing signalling events responsible for regulating key tumorigenic processes such as proliferation, inhibition of apoptosis, cell adhesion/motility, growth and survival. Present study evaluates the potential of N-(3-Ethynylphenyl)-6, 7-bis (2-methoxyethoxy) quinolin-4-amine as a new inhibitor for EGFR. We have explored the binding and inhibitory potential of the compound using molecular docking, structural interactions fingerprinting and molecular dynamics studies. The inhibitor exhibits extensive interactions with the EGFR catalytic site in the form of hydrogen bonds, pi-pi bond and salt bridges. It shows high specificity and binding affinity towards the protein. The compound can further be explored for its potential to serve in the diagnosis and treatment of HNSCC. The quantitative prediction provides a scope for future experimental testing, facilitating the understanding of the crosstalks between signalling pathways. © 2012 Bentham Science Publishers. Source


Gupta S.,Biotech Park | Misra G.,Biotech Park | Pant M.C.,University of Lucknow | Seth P.K.,Biotech Park
Journal of Molecular Modeling | Year: 2011

Protein-protein interactions play an important role in regulating the expression of huntingtin protein (htt). Expansion of polyglutamine tracts in htt results in neurodegenerative Huntington disease. Huntingtin interacting protein (HIP14) is an important interacting partner of htt and the altered interactions have been proposed to play an important role in disease progression. In the present study, an attempt has been made to explore the potential of several known Huntington inhibitors to inhibit HIP14. The docking studies have resulted in the identification of a novel binding site for these inhibitors distinct from the previously known ankyrin repeat domain. The results have been validated using geometry based docking transformations against the other binding pocket. The specificity of binding has been determined with high values of both accuracy and precision. Nine potential inhibitors obtained after screening belong to three distinct classes of compounds viz, carbohydrates (deoxy-glucose), alcohols (including phenolic scaffold) and tetracycline. The compounds form stable complex with protein exhibiting optimal intermolecular and Gibbs free energy. The hydrogen bonding and hydrophobic interactions predominantly contribute to the stability of these complexes. The present study identifies metoprolol, minocyclines and 18 F fluorodeoxyglucose as the best inhibitors that bind specifically to the new site. Therefore, these compounds can further be exploited for their potential to serve in the diagnosis and treatment of Huntington disease. The quantitative predictions provide a scope for experimental testing in future. © Springer-Verlag 2011. Source


Devi S.P.,Central Agricultural University | Kumaria S.,North - Eastern Hill University | Rao S.R.,North - Eastern Hill University | Tandon P.,Biotech Park
Tropical Plant Biology | Year: 2016

Carnivorous plants are among the curiosities of nature being different from the normal plants in their mode of nutrition. These plants have fascinated several researchers for centuries. They are also characterized by synthesis of bioactive compounds which are used as a mechanism for self defense. These compounds possess a broad spectrum of biological activities such as antiparasitic, antibacterial, insecticidal, fungicidal, anti-inflammatory, antipyretic, antiproliferative activities. Although, several antimicrobial drugs have been introduced during the recent decades, the problems of microbial infections resistant to synthetic pesticides still exist which necessitate the introduction of novel antimicrobial agents with additional modes of actions than the currently available therapeutic agents. Naphthoquinones are one of the most studied bioactive compounds which have been reported to inhibit the growth of proliferative cells and microbes. Efforts have been made to induce the biosynthesis of naphthoquinone in different species of carnivorous plants. It has been demonstrated that the accumulation of naphthoquinones in carnivorous plants was increased by injecting chitin into the plant tissues. Also, their biosynthesis could be enhanced by the incorporation of elicitors in in vitro cultures of plants. In the present review, we discuss the applications of naphthoquinones and its biosynthesis in carnivorous plants. © 2016 Springer Science+Business Media New York Source

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