Biotech Park

Lucknow, India

Biotech Park

Lucknow, India
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Bhattacharyya P.,North - Eastern Hill University | Bhattacharyya P.,University of KwaZulu - Natal | Kumaria S.,North - Eastern Hill University | Bose B.,North - Eastern Hill University | And 2 more authors.
Journal of Applied Research on Medicinal and Aromatic Plants | Year: 2017

Rumex nepalensis is an important medicinal herb found in high altitude regions of India. It is a rich source of various secondary metabolites and has a widespread use in various herbal preparations. The regeneration efficacy of both direct shoot organogenesis (DSO) and indirect shoot organogenesis (ISO) was experimentally tested and the highest regeneration frequency for DSO and ISO pathways were 87.2% and 91.2% respectively. The best rooting frequency was recorded in half-strength MS medium supplemented with 2. mg/l NAA. The plantlets after acclimatization were phenotypically similar to that of the mother plant. The clonal stability of the regenerated plants from both DSO and ISO pathways were determined using Start Codon Targeted Polymorphism (SCoT) and Random amplified polymorphic DNA (RAPD) marker techniques. Although RAPD technique detected lesser degree of variability within the cloned plants (both DSO and ISO), SCoT marker could detect comparatively higher degree of genetic variability thereby proving its efficacy over the conventional molecular markers. Regeneration methodology, plant part and solvent system significantly influenced the levels of various secondary metabolites like phenols, flavonoids, alkaloids and tannins. Simultaneously, the antioxidant assays (DPPH and FRAP) detected the antioxidant activity varied tissue specifically and a significant higher value of antioxidant activity was observed within the ISO-derived plants. Higher multiplication rate coupled with enhanced clonal stability and secondary metabolite production recorded in the present report ensure the efficacy of the protocol designed for the micropropagation of this important medicinal herb species. © 2017 Elsevier GmbH.

Diengdoh R.V.,North - Eastern Hill University | Kumaria S.,North - Eastern Hill University | Tandon P.,Biotech Park | Das M.C.,North - Eastern Hill University
South African Journal of Botany | Year: 2017

The present scenario of urbanization and commercialization has adversely affected orchid's population; as a consequence they are diminishing from the nature very rapidly. Paphiopedilum insigne (lady's slipper orchid) is one such orchid of horticultural importance. Apart from being listed as endangered in IUCN red data list; it finds a place in Appendix I of CITES in the global platform. In the present study, capsules < 180 days after pollination (DAP) were found to be immature and 180 DAP was found optimal for seed germination. Capsules > 240 DAP were found to have mature seeds that were treated prior to germination and TTC tested for viability. Seeds derived from 180 DAP capsules showed the highest germination of 88.5% in modified Burgeff medium (BG1) with initiation in 26 days. Incorporation of plant growth regulators like 5 μM kinetin (KN) + 10 μM indole-3-acetic acid (IAA) in 1/2 MS medium influenced the stage-wise development of the seedlings in a short duration. Mature seeds stored at − 196 °C for 360 days followed by pre-treatment with 3% NaOCl showed viability of 70.5% and recorded germination of 79.2%. Sixty-nine percent of the plantlets were successfully hardened and acclimatized in the green house. © 2017 SAAB

RAMNATH,North - Eastern Hill University | DUTTA A.K.,North - Eastern Hill University | DKHAR B.,North - Eastern Hill University | TANDON V.,Biotech Park | DAS B.,North - Eastern Hill University
Parasitology | Year: 2017

Phosphoenolpyruvate carboxykinase (PEPCK) is involved in glycolysis in the cestode parasite, Raillietina echinobothrida; whereas, it executes a gluconeogenic role in its host, Gallus domesticus. Because of its differing primary function in the cestode parasite and its host, this enzyme is regarded as a plausible anthelmintic target. Hence, the biological significance of PEPCK in the parasite was analysed using siRNA against PEPCK from R. echinobothrida (RePEPCK). In order to find out the functional differences between RePEPCK and GdPEPCK (PEPCK from its host, G. domesticus), PEPCK genes from both sources were cloned, over-expressed, characterized, and some properties of the purified enzymes were compared. RePEPCK and GdPEPCK showed a standard Michaelis-Menten kinetics with K mapp of 46.9 and 22.9 µ m, respectively, for phosphoenolpyruvate and K mapp of 15.4 µ m for oxaloacetate in GdPEPCK decarboxylation reaction. Here, we report antagonist behaviours of recombinant PEPCKs derived from the parasite and its host. In search of possible modulators for PEPCK, few phytoestrogens were examined on the purified enzymes and their inhibitory constants were determined and discussed. This study stresses the potential of these findings to validate PEPCK as the anthelmintic drug target for parasitism management. Copyright © Cambridge University Press 2017

Biswal D.K.,North - Eastern Hill University | Konhar R.,North - Eastern Hill University | Debnath M.,North - Eastern Hill University | Tandon V.,Biotech Park
Mitochondrial DNA Part B: Resources | Year: 2016

The complete mitogenome sequences of the intestinal fluke Fasciolopsis buski are presented for the first time in this study. It is 14,119 bp long and is thus the shortest trematode mitochondrial genome sequenced to date. The F. buski mtDNA genome has a close resemblance with F. hepatica and has a similar gene order tallying with that of other trematodes. The overall base composition of F. buski mitogenome is 17.89% for A, 9.16% for C, 27.59% for G and 45.36% for T, and has a GC content of 36.75%. The assembled mitogenome (GenBank accession number KX449331) consists of 12 protein-coding genes (PCGs), 22 transfer RNAs and two ribosomal RNA genes. The mtDNA for the intestinal fluke reported herein would help investigate Fasciolidae taxonomy and systematics with the aid of mtDNA NGS data. © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Gupta S.,Biotech Park | Misra G.,Hygia Institute of Pharmaceutical Education and Research | Pant M.C.,University of Lucknow | Seth P.K.,Biotech Park
Protein and Peptide Letters | Year: 2012

Head and neck squamous cell carcinoma (HNSCC) is a major cause of cancer related death. The epidermal growth factor receptor (EGFR) pathway is over expressed in HNSCC. EGFR regulates the HNSCC by inducing signalling events responsible for regulating key tumorigenic processes such as proliferation, inhibition of apoptosis, cell adhesion/motility, growth and survival. Present study evaluates the potential of N-(3-Ethynylphenyl)-6, 7-bis (2-methoxyethoxy) quinolin-4-amine as a new inhibitor for EGFR. We have explored the binding and inhibitory potential of the compound using molecular docking, structural interactions fingerprinting and molecular dynamics studies. The inhibitor exhibits extensive interactions with the EGFR catalytic site in the form of hydrogen bonds, pi-pi bond and salt bridges. It shows high specificity and binding affinity towards the protein. The compound can further be explored for its potential to serve in the diagnosis and treatment of HNSCC. The quantitative prediction provides a scope for future experimental testing, facilitating the understanding of the crosstalks between signalling pathways. © 2012 Bentham Science Publishers.

Devi S.P.,Central Agricultural University | Kumaria S.,North - Eastern Hill University | Rao S.R.,North - Eastern Hill University | Tandon P.,Biotech Park
Tropical Plant Biology | Year: 2016

Carnivorous plants are among the curiosities of nature being different from the normal plants in their mode of nutrition. These plants have fascinated several researchers for centuries. They are also characterized by synthesis of bioactive compounds which are used as a mechanism for self defense. These compounds possess a broad spectrum of biological activities such as antiparasitic, antibacterial, insecticidal, fungicidal, anti-inflammatory, antipyretic, antiproliferative activities. Although, several antimicrobial drugs have been introduced during the recent decades, the problems of microbial infections resistant to synthetic pesticides still exist which necessitate the introduction of novel antimicrobial agents with additional modes of actions than the currently available therapeutic agents. Naphthoquinones are one of the most studied bioactive compounds which have been reported to inhibit the growth of proliferative cells and microbes. Efforts have been made to induce the biosynthesis of naphthoquinone in different species of carnivorous plants. It has been demonstrated that the accumulation of naphthoquinones in carnivorous plants was increased by injecting chitin into the plant tissues. Also, their biosynthesis could be enhanced by the incorporation of elicitors in in vitro cultures of plants. In the present review, we discuss the applications of naphthoquinones and its biosynthesis in carnivorous plants. © 2016 Springer Science+Business Media New York

Intercontinental dislocations between tropical regions harboring two-thirds of the flowering plants have always drawn attention from taxonomists and biogeographers. One such family belonging to angiosperms is Orchidaceae with an herbaceous habit and high species diversity in the tropics. Here, we investigate the evolutionary and biogeographical history of the genus Cymbidium, which represents a monophyletic subfamily (Epidendroideae) of the orchids and comprises 50 odd species that are distinctly distributed in tropical to temperate regions. Much is not known about correlations among the level of CAM activity (one of the photosynthetic pathways often regarded as an adaptation to water stress in land plants), habitat, life forms, and phylogenetic relationships of orchids from an evolutionary perspective. A relatively well-resolved and highly supported phylogeny for Cymbidium orchids is reconstructed based on sequence analysis of ITS2 and matK regions from the chloroplast DNA available in public repositories viz. GenBank at NCBI.This study examines a genus level analysis by integrating different molecular matrices to existing fossil data on orchids in a molecular Bayesian relaxed clock employed in BEAST and assessed divergence times for the genus Cymbidium with a focus on evolutionary history of photosynthetic characters. Our study has enabled age estimations (45Ma) as well as ancestral area reconstruction for the genus Cymbidium using BEAST by addition of previously analyzed two internal calibration points.

Gupta S.,Biotech Park | Misra G.,Biotech Park | Pant M.C.,University of Lucknow | Seth P.K.,Biotech Park
Journal of Molecular Modeling | Year: 2011

Protein-protein interactions play an important role in regulating the expression of huntingtin protein (htt). Expansion of polyglutamine tracts in htt results in neurodegenerative Huntington disease. Huntingtin interacting protein (HIP14) is an important interacting partner of htt and the altered interactions have been proposed to play an important role in disease progression. In the present study, an attempt has been made to explore the potential of several known Huntington inhibitors to inhibit HIP14. The docking studies have resulted in the identification of a novel binding site for these inhibitors distinct from the previously known ankyrin repeat domain. The results have been validated using geometry based docking transformations against the other binding pocket. The specificity of binding has been determined with high values of both accuracy and precision. Nine potential inhibitors obtained after screening belong to three distinct classes of compounds viz, carbohydrates (deoxy-glucose), alcohols (including phenolic scaffold) and tetracycline. The compounds form stable complex with protein exhibiting optimal intermolecular and Gibbs free energy. The hydrogen bonding and hydrophobic interactions predominantly contribute to the stability of these complexes. The present study identifies metoprolol, minocyclines and 18 F fluorodeoxyglucose as the best inhibitors that bind specifically to the new site. Therefore, these compounds can further be exploited for their potential to serve in the diagnosis and treatment of Huntington disease. The quantitative predictions provide a scope for experimental testing in future. © Springer-Verlag 2011.

Saxena S.,Babasaheb Bhimrao Ambedkar University | Kesharwani R.K.,Babasaheb Bhimrao Ambedkar University | Singh V.,Babasaheb Bhimrao Ambedkar University | Singh S.,Biotech Park
International Journal of Bioinformatics Research and Applications | Year: 2013

Geminiviruses are single-stranded circular DNA viruses causing leaf curl disease in papaya crop. Post-Transcriptional Gene Silencing (PTGS), also known as RNAi, acts as a natural antiviral defence mechanism and plays a role in genome maintenance and development in plants. PTGS suppression by viruses makes the plant RNA silencing machinery inefficient. Three geminiviral genes namely AV2, AC2 and AC4 are found to play the role in suppression of RNA silencing. siRNA degrades the target mRNA in a homology-dependent manner. In-silico designing of siRNA against these three genes of geminiviruses infecting Carica papaya was done using bioinformatics tools. This strategy may provide PTGS by specifically targeting the viral genes involved in suppression of plant RNA silencing machinery. Copyright © 2013 Inderscience Enterprises Ltd.

PubMed | Biotech Park and University of Lucknow
Type: Journal Article | Journal: Bioinformation | Year: 2014

In this work, 3D model of D2 dopamine receptor was determined by comparative homology modeling program MODELLER. The computed models energy was minimized and validated using PROCHECK and Errat tool to obtain a stable model structure and was submitted in Protein Model Database (PMDB-ID: PM0079251). Stable model was used for molecular docking against Risperidone and their 15 derivatives using AutoDock 4.2, which resulted in energy-based descriptors such as Binding Energy, Ligand Efficiency, Inhib Constant, Intermol energy, vdW + Hbond + desolv Energy, Electrostatic Energy, Total Internal Energy and Torsional Energy. After that, we have built quantitative structure activity relationship (QSAR) model, which was trained and tested on Risperidone and their 15 derivatives having activity value pKi in M. For QSAR modeling, Multiple Linear Regression model was engendered using energy-based descriptors yielding correlation coefficient r2 of 0.513. To assess the predictive performance of QSAR models, different cross-validation procedures were adopted. Our results suggests that ligand-receptor binding interactions for D2 employing QSAR modeling seems to be a promising approach for prediction of pKi value of novel antagonists against D2 receptor.

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