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Stavanger, Norway

Pittman K.,University of Bergen | Pittman A.,University of Bergen | Karlson S.,University of Bergen | Cieplinska T.,University of Bergen | And 5 more authors.
Journal of Fish Diseases | Year: 2013

Mucous cell size and distribution were investigated in the skin of five salmon using a novel stereology-based methodology: one (48 cm) fish to test 15 tissue treatment combinations on measures of cell area and density on the dorsolateral region and, using the most suitable treatment, we mapped mucous cell differences between body regions on four (52 cm) salmon, comprising a male and a female on each of two diets. The section site, decalcification, embedding medium and plane of sectioning all impacted significantly on mucous cell size, whereas mucous cell density is more robust. There were highly significant differences in both mucosal density and mean mucous cell size depending on body site: the dorsolateral skin of the four salmon had significantly denser (about 8% of skin area) and larger (mean about 160 μm2) mucous cells, whereas the lowest mean density (about 4%) and smallest mean area (115 μm2) were found on the head. We found that 100 random measurements may be sufficient to distinguish differences >7 μm2 in mean mucous cell areas. The results further suggest that salmon exhibit a dynamic repeatable pattern of mucous cell development influenced by sex, diet and possibly strain and season. © 2012 Blackwell Publishing Ltd. Source


Nilsen M.M.,IRIS - International Research Institute of Stavanger | Meier S.,Norwegian Institute of Marine Research | Andersen O.K.,Biotatools AS | Hjelle A.,IRIS - International Research Institute of Stavanger
Marine Pollution Bulletin | Year: 2011

Proteomics is a new and promising approach to evaluate potential effects of pollution. In order to investigate if there is a direct link between the protein expression profiles obtained by the SELDI-TOF MS technology and effects observed at the organism level in fish, plasma samples from unexposed and 20. ppb alkylphenol exposed female Atlantic cod (Gadus morhua) with high phenotypic variation in gonadosomatic index (GSI) were analyzed by SELDI-TOF MS. Principle component analysis (PCA) showed that the major proteomic variation present in the dataset (i.e. 23.6%) could be significantly correlated to the individual variation in GSI, which indicates that SELDI-TOF MS data can reflect effects observed at higher levels of organization in fish. Further exploration of the other principal components revealed an additional proteomic pattern specific for the alkylphenol exposed females. Hence, this study supports the usefulness of SELDI-TOF MS as a proteomic tool in ecotoxicological research. © 2011 Elsevier Ltd. Source


Maeland Nilsen M.,IRIS - International Research Institute of Stavanger | Uleberg K.-E.,IRIS - International Research Institute of Stavanger | Uleberg K.-E.,University of Stavanger | Uleberg K.-E.,University of Bergen | And 5 more authors.
Journal of Proteomics | Year: 2011

SELDI-TOF MS has been demonstrated as a powerful tool for biomarker discovery. However, a major disadvantage of SELDI-TOF MS is the lack of direct identification of the discriminatory peaks discovered. We describe a novel experimental identification strategy where peptides/proteins captured to a weak cation exchange ProteinArray surface (CM10) are eluted, and thereafter identified by utilizing a sensitive LC-MS/MS (i.e. LTQ Orbitrap). A mixture of four known proteins was used to test the novel experimental approach described, and all four proteins were successfully identified. Additionally, a biomarker candidate previously discovered in plasma of Atlantic cod (Gadus morhua) by SELDI-TOF MS was identified. Thus, this study indicated that a combination of on-chip elution and a highly sensitive LC-MS/MS system can be an alternative approach to identify biomarker candidates discovered by use of SELDI-TOF MS. © 2011 Elsevier B.V. Source


Maeland Nilsen M.,IRIS - International Research Institute of Stavanger | Meier S.,Norwegian Institute of Marine Research | Larsen B.K.,Technical University of Denmark | Ketil Andersen O.,Biotatools AS | Hjelle A.,IRIS - International Research Institute of Stavanger
Ecotoxicology and Environmental Safety | Year: 2011

Compound-specific protein expression signatures (PESs) can be revealed by proteomic techniques. The SELDI-TOF MS approach is advantageous due to its simplicity and high-throughput capacity, however, there are concerns regarding the reproducibility of this method. The aim of this study was to define an estrogen-responsive PES in plasma of Atlantic cod (Gadus morhua) using the SELDI-TOF MS technique. Protein expression analysis of male cod exposed to 17Β-estradiol (E 2) showed that 27 plasma peaks were differentially expressed following exposure. The reproducibility of this result was evaluated by reanalyzing the samples six months later, and a significant change in expression was confirmed for 13 of the 27 peaks detected in the first analysis. The performance of the reproducible E 2-responsive PES, constituting these 13 peaks, was then tested on samples from juvenile cod exposed to 4-nonylphenol, North Sea oil, or North Sea oil spiked with alkylphenols. Principal component analysis revealed that nonylphenol-exposed cod could be separated from unexposed cod based on the E 2-responsive PES, indicating that the PES can be used to assess estrogenic exposure of both juvenile and adult specimens of cod. A targeted antibody-assisted SELDI-TOF MS approach was carried out in an attempt to identify the E 2-responsive peaks. Results indicated that 2 peaks were fragments of the well-known biomarkers VTG and/or ZRP. In this study, the SELDI-TOF MS technology has shown its potential for defining compound-specific PESs in fish. Nevertheless, thorough validation of reproducibility, specificity and sensitivity of a PES is required before it can be applied in environmental monitoring. © 2011 Elsevier Inc. Source


Patent
Biotatools As | Date: 2012-08-10

The effect of pollution in an aquatic mass is monitored. A living sessile organism (

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