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Shujun L.,Beijing Aeronautical Science and Technology Research Institute | Shujun L.,Biosystems International
AMA, Agricultural Mechanization in Asia, Africa and Latin America | Year: 2016

Agricultural machinery, as the material basis for developing modern agriculture, is an indispensable means for improving agricultural production efficiency, transforming the agricultural development pattern, enhancing the comprehensive competitiveness of agriculture, and ensuring national food security and effective supply of agricultural products. In recent years, in the process of rapid socio-economic growth in rural China, with the effective implementation of agriculture and farmers-friendly policies, agricultural machinery has become a most dynamic industry in the whole machinery sector in the country with daily improved research capacities and industrial technologies, and greatly enlarged product varieties and industrial scale. Under the new circumstances in the country, with the urbanization being accelerated and the rural labor increasingly transferred, development of farm machines suitable for the national conditions in the process of agricultural mechanization shall be the fundamental approach to solve the problem of "Who farm and how to farm", in order to meet the requirements in building up the modern agriculture with a sound mode and in a vertical direction. In this review, the paper describes the achievements made in the agricultural machinery and agricultural mechanization, the implementation of the key Sci-Tech planning projects, the innovation of basic applied technologies and key common products, as well as the establishment of four-in-one industrial chain innovation system covering projects, bases, personnel and mechanism in China. It also summarizes the development of the major technologies and products from 7 perspectives such as precision farming and intelligent technologies, farm power and tractors, multifunctional working machines, harvesters, livestock machinery, agro-products and food processing equipment, and agro-forest biomass utilization equipment. In conclusion, the paper indicates the future trends and priorities in the sci-tech development of the agricultural machinery in China from the following four aspects: Strengthening basic research and advancing original innovative capabilities, developing key common technologies and equipment for sound supply of domestic agricultural machinery, upgrading the productivity and quality of high-end equipment, and accelerating going-global for agricultural engineering in the process of the internationalization.

Varadi C.,Debrecen University | Mittermayr S.,Debrecen University | Szekrenyes A.,Debrecen University | Kadas J.,Biosystems International | And 3 more authors.
Electrophoresis | Year: 2013

A CE-based method was introduced to compare the N-glycosylation profile of haptoglobin in normal and pathologic conditions. To assess the biomarker potential of glycosylation changes in various lung diseases, haptoglobin was isolated from plasma samples of healthy, pneumonia, chronic obstructive pulmonary disease, and lung cancer patients by means of two haptoglobin-specific monoclonal antibodies. Haptoglobin N-glycans were then enzymatically released, fluorescently labeled, and profiled by CE. Disease-associated changes of core and antennary fucosylation were identified by targeted exoglycosidase digestions and their levels were compared in the different patient groups. Terms such as core- and arm-fucosylation degree, as well as branching degree, were introduced for easier characterization of the changes and statistical analysis was used to examine which structures were responsible for the observed differences. Increased level of α1-6 fucosylated tri-antennary glycans was found in all disease groups compared to the control. Elevated amounts of core- and arm-fucosylation on tetra-antennary glycans were detected in the lung cancer group compared to the chronic obstructive pulmonary disease group. A larger scale study is necessary to confirm and validate these preliminary findings in the glycosylation changes of haptoglobin, so could then be used as biomarkers in the diagnosis of malignant and inflammatory lung diseases. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Guergova-Kuras M.,Biosystems International
Molecular & cellular proteomics : MCP | Year: 2011

A challenge in the treatment of lung cancer is the lack of early diagnostics. Here, we describe the application of monoclonal antibody proteomics for discovery of a panel of biomarkers for early detection (stage I) of non-small cell lung cancer (NSCLC). We produced large monoclonal antibody libraries directed against the natural form of protein antigens present in the plasma of NSCLC patients. Plasma biomarkers associated with the presence of lung cancer were detected via high throughput ELISA. Differential profiling of plasma proteomes of four clinical cohorts, totaling 301 patients with lung cancer and 235 healthy controls, identified 13 lung cancer-associated (p < 0.05) monoclonal antibodies. The monoclonal antibodies recognize five different cognate proteins identified using immunoprecipitation followed by mass spectrometry. Four of the five antigens were present in non-small cell lung cancer cells in situ. The approach is capable of generating independent antibodies against different epitopes of the same proteins, allowing fast translation to multiplexed sandwich assays. Based on these results, we have verified in two independent clinical collections a panel of five biomarkers for classifying patient disease status with a diagnostics performance of 77% sensitivity and 87% specificity. Combining CYFRA, an established cancer marker, with the panel resulted in a performance of 83% sensitivity at 95% specificity for stage I NSCLC.

Szekrenyes A.,Debrecen University | Roth U.,Qiagen | Kerekgyarto M.,Debrecen University | Szekely A.,Debrecen University | And 3 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

Capillary gel electrophoresis (CGE) in the presence of sodium dodecyl sulfate (SDS) is a well-established and widely used protein analysis technique in the biotechnology industry, and increasingly becoming the method of choice that meets the requirements of the standards of International Conference of Harmonization (ICH). Automated single channel capillary electrophoresis systems are usually equipped with UV absorbance and/or laser-induced fluorescent (LIF) detection options offering general applicability and high detection sensitivity, respectively; however, with limited throughput. This shortcoming is addressed by the use of multicapillary gel electrophoresis (mCGE) systems with LED-induced fluorescent detection (LED-IF), also featuring automation and excellent detection sensitivity, thus widely applicable to rapid and large-scale analysis of biotherapeutics, especially monoclonal antibodies (mAb). The methodology we report in this paper is readily applicable for rapid purity assessment and subunit characterization of IgG molecules including detection of non-glycosylated heavy chains (NGHC) and separation of possible subunit variations such as truncated light chains (Pre-LC) or alternative splice variants. Covalent fluorophore derivatization and the mCGE analysis of the labeled IgG samples with multi-capillary gel electrophoresis are thoroughly described. Reducing and non-reducing conditions were both applied with and without peptide N-glycosidase F mediated deglycosylation. © 2012 Springer-Verlag.

Hanssen I.M.,Scientia Terrae Research Institute | van Esse H.P.,Wageningen University | Ballester A.-R.,Biosystems International | Hogewoning S.W.,Wageningen University | And 5 more authors.
Plant Physiology | Year: 2011

Pepino mosaic virus (PepMV) is a highly infectious potexvirus and a major disease of greenhouse tomato (Solanum lycopersicum) crops worldwide. Damage and economic losses caused by PepMV vary greatly and can be attributed to differential symptomatology caused by different PepMV isolates. Here, we used a custom-designed Affymetrix tomato GeneChip array with probe sets to interrogate over 22,000 tomato transcripts to study transcriptional changes in response to inoculation of tomato seedlings with a mild and an aggressive PepMV isolate that share 99.4% nucleotide sequence identity. The two isolates induced a different transcriptomic response, despite accumulating to similar viral titers. PepMV inoculation resulted in repression of photosynthesis. In addition, defense responses were stronger upon inoculation with the aggressive isolate, in both cases mediated by salicylic acid signaling rather than by jasmonate signaling. Our results furthermore show that PepMV differentially regulates the RNA silencing pathway, suggesting a role for a PepMV-encoded silencing suppressor. Finally, perturbation of pigment biosynthesis, as shown by differential regulation of the flavonoid and lycopene biosynthesis pathways, was monitored. Metabolite analyses on mature fruits of PepMV-infected tomato plants, which showed typical fruit marbling, revealed a decrease in carotenoids, likely responsible for the marbled phenotype, and an increase in alkaloids and phenylpropanoids that are associated with pathogen defense in the yellow sectors of the fruit. © 2011 American Society of Plant Biologists.

Machida T.,Biosystems International
AMA, Agricultural Mechanization in Asia, Africa and Latin America | Year: 2011

The Japan Association of International Commission of Agriculture and Biosystems Engineering (JAICABE) was established in 1984 with approval of six academic societies and one association for contributing to the development of agricultural engineering technology. JAICABE held annual conventions and symposiums as academic projects, in addition to awarding ceremonies for fellow recognitions. It also participated in the activities of International Commission of Agricultural Engineering (CIGR) as the representative society of the country and sent its members as the officials to CIGR, along with committee members to the respective sections from Section 1 to Section 7. JAICABE worked jointly with the CIGR branch society at the Science Council of Japan to make proposals to the government for agricultural engineering and held international academic symposiums and workshops in cooperation with the council.

Wang D.,Northeastern University | Hincapie M.,Northeastern University | Guergova-Kuras M.,Biosystems International | Kadas J.,Biosystems International | And 2 more authors.
Journal of Proteome Research | Year: 2010

A mass spectrometric (MS)-based strategy for antigen (Ag) identification and characterization of globally produced monoclonal antibodies (mAbs) is described. Mice were immunized with a mixture of native glycoproteins, isolated from the pooled plasma of patients with nonsmall cell lung cancer (NSCLC), to generate a library of IgG-secreting hybridomas. Prior to immunization, the pooled NSCLC plasma was subjected to 3-sequential steps of affinity fractionation, including high abundant plasma protein depletion, glycoprotein enrichment, and polyclonal antibody affinity chromatography normalization. In this paper, to demonstrate the high quality of the globally produced mAbs, we selected 3 mAbs of high differentiating power against a matched, pooled normal plasma sample. After production of large quantities of the mAbs from ascites fluids, Ag identification was achieved by immunoaffinity purification, SDS-PAGE, Western blotting, and MS analysis of in-gel digest products. One antigen was found to be complement factor H, and the other two were mapped to different subunits of haptoglobin (Hpt). The 2 Hpt mAbs were characterized in detail to assess the quality of the mAbs produced by the global strategy. The affinity of one of the mAbs to the Hpt native tetramer form was found to have a K D of roughly 10-9 M and to be 2 orders of magnitude lower than the reduced form, demonstrating the power of the mAb proteomics technology in generating mAbs to the natural form of the proteins in blood. The binding of this mAb to the β-chain of haptoglobin was also dependent on glycosylation on this chain. The characterization of mAbs in this work reveals that the global mAb proteomics process can generate high-quality lung cancer specific mAbs capable of recognizing proteins in their native state. © 2010 American Chemical Society.

Agency: European Commission | Branch: H2020 | Program: SME-1 | Phase: PHC-12-2015-1 | Award Amount: 71.43K | Year: 2015

Cancer incidence is continuously increasing, making it a major concern of European governments. Cheaper, more sensitive and specific diagnostic tests are needed to foster early cancer diagnosis, thereby alleviating the impact of cancer on citizens lives and reducing overall cancer care costs. BioSystems International Kft. (BSI) developed a diagnostics technology based on plasma protein epitome biomarker panels, with promising results to meet these needs for several cancers. BSI novel biomarkers-based in vitro diagnostics will bring better health outcomes for the patient as earlier detection will increase survival and will reduce overall care costs diagnosis contributing to the sustainability of the health care system. The CANDLE project objective is to consolidate the business plan and to complete clinical validation of the biomarker panels for breast and colon cancers, together representing 26.8 % of all cancer incidences in the EU. Clinically validated results obtained in CANDLE will enable BSI to seize the business opportunity and to maximize return on investments through higher royalties. BSI will license its cancer diagnostics intellectual property (IP) to fully integrated diagnostics companies who will get a competitive advantage by putting more cost-effective diagnostic products on the market. BSI already licensed its candidate diagnostic for lung cancer to a major European diagnostics company, demonstrating proof-of-concept for this business model. CANDLE will enable BSI to licence its IP after clinical validation at much better terms than for lung cancer, licensed at an earlier qualification stage. The global diagnostics market for cancers was estimated at 30 billion Euros in 2012. BSIs IP-based diagnostics are expected to seize 4 % of this market within 10 years, resulting in more than 49.2 M of license revenues for BSI. CANDLE will boost BSIs competitiveness and profitability, enabling the company to grow and to increase its innovation activity.

News Article | July 19, 2010

Following the discovery of novel biomarkers for lung cancer, the company launched the development of a new blood test for the detection of lung cancer in May, 2009. This new funds, which were provided by the company’s existing investors, will be used to complete development of the test and bring it to the market in 2011. Commenting on the financing, Jean-Pierre Tirouflet, Chief Executive Officer of Biosystems International, said: “The renewed commitment by our shareholders demonstrates their confidence in our lung cancer program. These funds will help to accelerate our entry to market”.

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