Miron D.,Federal University of Rio Grande do Sul |
Cornelio R.,Development Laboratory of Quimayra Farmaceutica |
Troleis J.,Bioscience Institute |
Mariath J.,Bioscience Institute |
And 3 more authors.
European Journal of Pharmaceutical Sciences | Year: 2014
This work aimed to evaluate the effect of different substances on the permeation of geraniol through bovine hoof membranes. Different penetration enhancers were able to increase the permeability up to 25 times compared to control. It was demonstrated that acetilcysteine in association with ascorbic acid increased the permeation, even in acid formulations. In addition, some antifungal drugs were incorporated into a gel formulation of HPMC containing acetylcysteine 5% and ascorbic acid 0.2% and then the permeation coefficient through bovine hoof membranes was evaluated. The relationship between permeability and molecular weight was established for fluconazole, miconazole, terbinafine, butenafine, geraniol and nerol. Geraniol and nerol, the antifungals with lower molecular weight, had the better permeability results. Permeability coefficients for nail plates were estimated and geraniol demonstrated similar or even better efficacy index values against T. rubrum, T. menthagrophytes and M. canis compared with terbinafine and miconazole. © 2013 Elsevier B.V. All rights reserved.
News Article | December 1, 2015
« ARPA-E awards U-M $1.9M to develop advanced low-cost high-efficiency engine; boosting, highly dilute combustion and 48V system | Main | BMW delivers 50 i3 EVs to city of Leipzig » A study by researchers at the University of São Paulo’s Bioscience Institute (IB-USP) in Brazil, with colleagues at the Ohio State University, has found that the rising atmospheric concentration of CO is beneficial for the physiology of sorghum, an economically and nutritionally important crop grown worldwide. An open-access paper on their work is published in the journal Plant Physiology. The ability of sorghum to benefit from rising CO levels is due to a peculiarity of photosynthesis in the family of C grasses, which include sugarcane and maize as well as sorghum. The researchers found that when sorghum plants were kept in a low-humidity environment, CO not only protected them against drought but also promoted a systemic adjustment in their metabolism that led their seeds to accumulate 60% more protein. The discovery contributes to a better understanding of the impact of climate change on sorghum and other grasses such as sugarcane and maize. According to Marcos Silveira Buckeridge, the principal investigator for the project “Using systems biology approach to develop a model for whole plant functioning”, the study is the first systematic analysis of the effects on sorghum of high concentrations of CO combined with drought to consider the interactions between different organs of the plant. The project is supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) and Microsoft Research. While most research in the field focuses on specific parts of plants, such as leaves, stems or roots, we set out to understand sorghum as a system. We studied the interactions among its organs when subjected to water stress combined with high levels of CO . Whole-plant metabolism studies of this kind improve our understanding of the implications of modifying several genes or introducing an entire metabolic pathway into one organ, such as a system in the stem that accelerates water transport. This requires investigating what happens not just in the stem but also in leaves, flowers and seeds, producing a broader picture without losing the specific focus on the more reduced level of processes, i.e., the plant’s biochemistry. Sorghum is genetically very similar to sugarcane. It is an excellent model for the study of grasses that are economically more important to Brazil because of the simplicity of its genome, which has been completely sequenced and is available to the international scientific community. The ability of sorghum to benefit from rising CO levels is due to a peculiarity of photosynthesis in the family of C grasses, which include sugarcane and maize as well as sorghum. C photosynthesis uses a supplementary method of CO uptake in which a 4-carbon molecule is formed instead of the two 3-carbon molecules involved in the more widespread C process. To analyze the interactions among plant organs during the process, Buckeridge’s team at IB-USP, in collaboration with researchers at the Ohio State University in the United States, performed metabolomics studies on sorghum to investigate all the metabolites produced or modified under specific conditions, as well as gene functions and interrelations, gene and protein expression and regulation, and the metabolic output of the system. Sorghum plants were grown under elevated CO and drought conditions for 120 days during the grain formation and filling phase. Leaf photosynthesis, respiration and stomatal conductance (the rate of CO exchange between leaf stomata and the air) were measured 90 days and 120 days after planting. Plants were harvested at the end of each period, and the biomass and intracellular primary metabolites of leaves, culm, root, prop roots and grains were evaluated. The researchers found that elevated CO reduced stomatal conductance, leading to increased water use efficiency. Although minor physiological effects were observed, growing sorghum under high levels of atmospheric CO was found to mitigate the loss of grain quality caused by low humidity during the filling phase thanks to a delay in physiological and metabolic responses to drought. To our knowledge, this is the first study to analyse the simultaneous metabolic responses of the different organs of a plant grown in these conditions. It also shows for the first time how changes in each organ can affect grain composition in sorghum. The findings pave the way for a deeper understanding of the genes responsible for this resilience to climate change, and in particular of how the plant's organs can interact to improve seed production.
News Article | November 22, 2016
VGXI was honored to host U.S. Congressman Pete Olson and several key industry leaders for a roundtable discussion on the state of biotech and innovation in Texas. Attendees included President of the Biotechnology Innovation Organization (BIO), Jim Greenwood, as well as Tom Kowalski, President of the Texas Healthcare and Bioscience Institute, and Ann Tanabe, CEO of BioHouston. The visitors toured VGXI’s state-of-the-art cGMP plasmid DNA manufacturing facility and met with the CEO of VGXI and GeneOne Life Science, Young Park. The roundtable discussion that followed focused on funding and policy issues facing the biotech community, and the important role of innovative companies like VGXI. VGXI is the only facility in the world to have manufactured DNA vaccines for Ebola, MERS, and Zika. In July of 2016 VGXI’s parent company GeneOne Life Science announced the start of a Phase-1 trial to evaluate the Zika DNA vaccine (GLS-5700), which has been demonstrated in animal models to provide 100% protection from the virus. This trial set a record as one of the fastest development timelines from initial concept of the vaccine to FDA approval for human testing. Roundtable attendees noted the exceptional safety profile and rapid manufacturing timeline for DNA vaccines, making them a smarter and more cost effective approach to emerging diseases. “Establishing commercial scale manufacturing capability for plasmid DNA within the US will ensure we are ready to support production and widespread distribution of these vaccines in response to future outbreaks,” stated GeneOne CEO Young Park. ABOUT VGXI, INC. With over 15 years of experience, VGXI, Inc. is a leading provider of plasmid DNA manufacturing and development services for DNA vaccine and gene therapy research. The company has an outstanding track record of success in manufacturing plasmid products under cGMP conditions for clinical trials in the US, EU, Asia and Australia, and its cGMP and non-GMP products have passed rigorous reviews by several international regulatory agencies. VGXI’s ability to work with unique requirements and create custom manufacturing solutions is based on its patented manufacturing process, flexible cGMP production facility, and experienced development team. VGXI, Inc. is a wholly-owned subsidiary CMO of GeneOne Life Science, Inc. To learn more about VGXI, visit http://www.vgxii.com/about/overview. ABOUT GENEONE LIFE SCIENCE, INC. GeneOne Life Science Inc. is an international DNA vaccine developer and leading contract manufacturer of DNA plasmid-based agents for preclinical and clinical trials for global companies and institutions. It researches and develops DNA vaccines to prevent and treat incurable diseases in South Korea and internationally. The company is headquartered in Seoul, South Korea. VGXI, Inc., GeneOne’s wholly-owned manufacturing subsidiary located in Texas, is the largest pure-play cGMP DNA plasmid manufacturing facility in the world. ABOUT BIO Biotechnology Innovation Organization (BIO) is the world's largest trade association representing nearly 1,000 biotechnology companies, academic institutions, state biotechnology centers and related organizations across the United States and in more than 30 other nations. BIO members are involved in the research and development of innovative healthcare, agricultural, industrial and environmental biotechnology products. ABOUT THBI Texas Healthcare and Bioscience Institute is the Texas Policy voice for healthcare and bioscience and the only provider of statewide resources to our members and the industry. With a focus on using advocacy as a tool to create a more favorable environment for the life sciences, THBI works with government and industry leaders to attract new participants in the life sciences to Texas and to promote effective government legislation on behalf of the industry. ABOUT BIOHOUSTON BioHouston, Inc. is a non-profit corporation founded by Houston area academic/research institutions, leading a broad effort to establish the Houston region as a vigorous global competitor in life science and biotechnology commercialization. Our mission is to create an environment that will stimulate technology transfer and research commercialization, thereby generating economic wealth for the Houston region and making it a global competitor in life science commercialization. BioHouston’s activities provide the greatest leverage in making the Houston region a world-class competitor in the life science industry. Cautionary Factors That May Affect Future Results Materials in this press release contain information that includes or is based upon forward-looking statements within the meaning of the Securities Litigation Reform Act of 1995. Forward-looking statements relate to expectations or forecasts of future events. These statements can be identified by the fact that they do not relate strictly to historical or current facts. They include words such as “anticipate,” “estimate,” “expect,” “project,” “intend,” “plan,” “believe,” and other words and terms of similar meaning in connection with a discussion of potential future events, circumstances or future operating or financial performance. In particular, these include statements relating to future actions, prospective products or product approvals, future performance or results of current and anticipated products, sales efforts, expenses, the outcome of contingencies such as legal proceedings, and financial results. Any or all of our forward-looking statements here or in other publications may turn out to be incorrect. They can be affected by inaccurate assumptions or by known or unknown risks and uncertainties. Many such factors will be important in determining our actual future results. Consequently, no forward-looking statement can be guaranteed, and forward-looking statements may be adversely affected by factors, including general market conditions, competitive product development, product availability, current and future branded and generic competition, federal and state regulations and legislation, manufacturing issues, timing of the elimination of trade buying, patent positions, litigations and investigations. Our actual results may vary materially, and there are no guarantees about the performance or valuation of GeneOne stock. It is also important to read the disclosure notice contained in many of the individual GeneOne documents available on the website, as many contain important information on such cautionary factors as of the date of the individual document. We undertake no obligation to correct or update any forward-looking statements, whether as a result of new information, future events or otherwise. You are advised, however, to consult any further disclosures we make on related subjects in our reports.
News Article | November 17, 2015
The park sprawling on an area of almost one hectare, is the focal point for research, learning, reference as well as recreation. Complete with a nursery, it draws visitors who want to get the plants or herbs for the purpose of traditional treatment. Formerly a rubber plantation, it was managed by Unit Ladang Universiti in the early 1980s before it was handed over to Bioscience Institute (IBS) in 1996 for the purpose of research activity. IBS Agriculture Officer, Rishzuan Talib said the park is divided into 11 zones based on its functions, usage and species of plants. The zones are Ginger, Medicinal Plants, Ferns, Traditional Vegetables, Aquatic Plants, Wild Orchids, Pitcher Plants, Aroid, Spices, Aromatic Plants and Forest Fruits. The focus, however, is mainly on herbal plants. At the Medicinal Plants zone, there are about 200 species of native and exotic plants that could be found in the country such as Mata Pelanduk (Ardisiacrenata) and Lemba (Molineriacapitulata). Rishzuan said iconic plants at the park were Keladi Murai or Belimbing Tanah (Taccaintergrifolia), known for their medicinal value said to be able to treat diabetes and high blood pressure. Other medicinal plants available at the park include Tongkat Ali (Eurycomalongifolia), Kacip Fatimah (Labisiapumila), Tongkat Ali Hitam (Polyathiabullata King), Tunjuk Langit (Helminthostachyszeylanica), Pecah Kaca or PecahBeling (Strobilanthescrispa), Putat (Baringtoniaracemosa ), Mahkota Dewa (Phaleriamacrocarpa), Senduduk Putih (Melastomadecemfidum), Belalai Gajah (Clinacanthus Nutans) and HempeduBumi (Andrographispaniculata). He said most of the species available at Medicinal Plants Zone were those obtained from forests in Peninsula Malaysia. He also said the species possessed medicinal benefits as an alternative to modern medicines. There were those that were rare and very difficult to find. At Ginger zone, there are about 100 species which make up half of the total number of species that could be found in Malaysia, including Tepus (Zingiberspectabile) and Kantan Hutan (Etlingeraterengganuensis). There are about 50 species of traditional vegetables at the Traditional Vegetables zone, such as Daun Gajus (Anacardiumoccidantale) and Daud Salam (Syzgiumpolyanthum), obtained from jungles and vegetable farms. Over at the Aromatic Plants Zone, there are about 50 native and exotic species. The fragrance from these plants is spread through various important components such as the flowers, leaves, barks, fruits, saps and roots like those of Cempaka (Micheliachampaca) and Hidung Babi (Rothmaniamacrophylla). Rishzuan added that the Conservatory Park was part of Edu-Park UPM's programmes where three modules were introduced for visitors – Explore The Park, Herbal Spa and Take Me Home which can be obtained at a minimal rate. Under Explore The Park module, visitors will be taken through a special passage around the park by guides who will be briefing them about the plants available. Each tree is tagged with its scientific name, local name as well as a description of its usage. The trees are marked with an ID and the information is kept in a database which could be obtained by surfing its website. There is a free wi-fi here. Visitors have the choice to follow the Herbal Spa module where a demonstration on the use of herbal plants for bathing is conducted. It is not only invigorating but helps to stimulate blood circulation and is ideal for those who have just given birth. He added that seven types of leaves that could be used for bathing including limau kasturi, pokok lemuni, serai wangi, lengkuas and daun kantan were normally cut into pieces first before they were boiled in an earthen pot. The leaves will give such a refreshing aroma. The product are sold in the form of sachet at RM30 for each box which contain five sachets. Each sachet can be used twice. Meanwhile, under the Take Me Home module, children will be entertained to a floral drawing activity while the adults are given a demonstration on planting and growing of herbs. Explore further: Herbal medicine through an evolutionary lens
Maioli M.,University of Sassari |
Maioli M.,Italian National Institute of Biosystems and Biostructures |
Rinaldi S.,Rinaldi Fontani Institute |
Rinaldi S.,University of Florence |
And 11 more authors.
Cell Transplantation | Year: 2012
Radiofrequency (RF) waves from Wi-Fi (wireless fidelity) technologies have become ubiquitous, with Internet access spreading into homes, and public areas. The human body harbors multipotent stem cells with various grading of potentiality. Whether stem cells may be affected by Wi-Fi RF energy remains unknown. We exposed mouse embryonic stem (ES) cells to a Radio Electric Asymmetric Conveyer (REAC), an innovative device delivering Wi-Fi RF of 2.4 GHz with its conveyer electrodes immersed into the culture medium. Cell responses were investigated by real-time PCR, Western blot, and confocal microscopy. Single RF burst duration, radiated power, electric and magnetic fields, specific absorption rate, and current density in culture medium were monitored. REAC stimulation primed transcription of genes involved in cardiac (GATA4, Nkx-2.5, and prodynorphin), skeletal muscle (myoD) and neuronal (neurogenin1) commitment, while downregulating the self renewal/pluripotency-associated genes Sox2, Oct4, and Nanog. REAC exposure enhanced the expression of cardiac, skeletal, and neuronal lineage-restricted marker proteins. The number of spontaneously beating ES-derived myocardial cells was also increased. In conclusion, REAC stimulation provided a "physical milieu" optimizing stem cell expression of pluripotentiality and the attainment of three major target lineages for regenerative medicine, without using chemical agonists or vector-mediated gene delivery. © 2012 Cognizant Comm. Corp.
Casadei A.,Casadei Clinic |
Epis R.,Bioscience Institute |
Ferroni L.,University of Padua |
Tocco I.,University of Padua |
And 7 more authors.
Journal of Biomedicine and Biotechnology | Year: 2012
Adipose tissue pathologies and defects have always represented a reconstructive challenge for plastic surgeons. In more recent years, several allogenic and alloplastic materials have been developed and used as fillers for soft tissue defects. However, their clinical use has been limited by further documented complications, such as foreign-body reactions potentially affecting function, degradation over time, and the risk for immunogenicity. Tissue-engineering strategies are thus being investigated to develop methods for generating adipose tissue. This paper will discuss the current state of the art in adipose tissue engineering techniques, exploring the biomaterials used, stem cells application, culture strategies, and current regulatory framework that are in use are here described and discussed. © 2012 Alessandro Casadei et al.
Ferroni L.,University of Padua |
Gardin C.,University of Padua |
Tocco I.,University of Padua |
Epis R.,Bioscience Institute |
And 3 more authors.
Advances in Biochemical Engineering/Biotechnology | Year: 2013
Adult human stem cells have gained progressive interest as a promising source of autologous cells to be used as therapeutic vehicles. Particularly, mesenchymal stem cells (MSCs) represent a great tool in regenerative medicine because of their ability to differentiate into a variety of specialized cells. Among adult tissues in which MSCs are resident, adipose tissue has shown clear advantages over other sources of MSCs (ease of surgical access, availability, and isolation), making adipose tissue the ideal large-scale source for research on clinical applications. Stem cells derived from the adipose tissue (adipose-derived stem cells = ADSCs) possess a great and unique regenerative potential: they are self-renewing and can differentiate along several mesenchymal tissue lineages (adipocytes, osteoblasts, myocytes, chondrocytes, endothelial cells, and cardiomyocytes), among which neuronal-like cells gained particular interest. In view of the promising clinical applications in tissue regeneration, research has been conducted towards the creation of a successful protocol for achieving cells with a well-defined neural phenotype from adipose tissue. The promising results obtained open new scenarios for innovative approaches for a cell-based treatment of neurological degenerative disorders. © Springer-Verlag Berlin Heidelberg 2012.
News Article | December 1, 2016
Collaboration in research and development between academia and industry has been the main driver for the development of innovative technologies for many years and should not be overlooked. The relationship between these two institutions should be a symbiotic one, according to Joy Goswami, assistant director at the Office of Economic Innovation and Partnership at the University of Delaware, who spoke at the second annual R&D 100 Conference in Washington, D.C., last month in a session titled “University-Industry Relations: Nurturing a Culture of Partnerships.” “Since industry and university are the two most powerful engines that can generate innovation, it would be obvious that if the two entities can work in conjunction, the resulting outcome would be enormous and could push the frontiers of innovation in a major way,” Goswami told R&D Magazine in an exclusive post-presentation interview. According to Goswami, collaborative benefits to a university include— increased opportunities for research funding; being a potential source for receiving monetary rewards that may go back to unrestricted R&D; providing an industrial connection leading to sponsored research funds; opportunities for consulting; assistance in student success via internships and employment opportunities; and provision of PR and prestige to its affiliated university. As for industry benefits from a relationship with a university—receipt of federal funding through governmental collaborative funding initiatives; cost savings via student hires as interns and faculty consultants; commercialization of university-based technologies for commercial and financial gain; subcontracting R&D project to university (due to lack of in-house infrastructure or expertise in the industry; enhancement of corporate image. So with all of these benefits to both entities, why are there still so many challenges associated with bridging cultural and communication divides between academia and the industry that has remained a constant impediment in fostering such collaborations? According to Goswami, the challenge is mostly due to the cultural divide associated with conducting R&D in the two organizations. Mainly because in academia—research priorities are set by an investigator, whereas in industry, these same priorities are set by management. Academia seeks grants, while industry seeks profits. In academia patenting is driven by publications, whereas in industry, patenting is driven by business decisions. These are just some of the differences between the two. So what could be done to bridge this great divide between the two institutions? While it’s not easy feat to bridge this divide between two institutions that conduct business so differently, it is possible, and Goswami mentioned several success stories during his presentation. One of these success stories included The Energy Bioscience Institute in Berkeley, Calif., which established a partnership with an industry that impacted its teaching and learning. U.K. tech company Imperial Innovations scored a relationship with academia that develops new funding streams for universities. The list goes on. Some of the ingredients to make a collaboration a reality include—instigating good leadership in both parties that “understand” each other’s situations and conditions; putting the right people in charge; establishing long-term strategic partnerships with built-in flexibility; starting with a shared vision and developing a strategy; encouraging cross-fertilization of ideas; not getting hung up on intellectual property; and by promoting a multidisciplinary approach to research and learning. “The number one reason for harboring more industry-university relations, in my opinion, would be to support innovation that, in turn, would stimulate greater economic growth for its society,” Goswami concluded.
Maioli M.,University of Sassari |
Maioli M.,University of Bologna |
Santaniello S.,University of Sassari |
Montella A.,University of Sassari |
And 13 more authors.
PLoS ONE | Year: 2010
Background: Development of molecules chemically modifying the expression of crucial orchestrator(s) of stem cell commitment may have significant biomedical impact. We have recently developed hyaluronan mixed esters of butyric and retinoic acids (HBR), turning cardiovascular stem cell fate into a high-yield process. The HBR mechanism(s) remain still largely undefined. Methodology/Principal Findings: We show that in both mouse embryonic stem (ES) cells and human mesenchymal stem cells from fetal membranes of term placenta (FMhMSCs), HBR differentially affected the patterning of Smad proteins, one of the major conductors of stem cell cardiogenesis. Real-time RT-PCR and Western blot analyses revealed that in both cell types HBR enhanced gene and protein expression of Smad1,3, and 4, while down-regulating Smad7. HBR acted at the transcriptional level, as shown by nuclear run-off experiments in isolated nuclei. Immunofluorescence analysis indicated that HBR increased the fluorescent staining for Smad1,3, and 4, confirming that the transcriptional action of HBR encompassed the upregulation of the encoded Smad proteins. Chromatin immune precipitation and transcriptional analyses showed that HBR increased the transcription of the cardiogenic gene Nkx-2.5 through Smad4 binding to its own consensus Smad site. Treatment of mouse ES cells and FMhMSCs with HBR led to the concomitant overexpression of both Smad4 and asarcomeric actinin. Smad4 silencing by the aid of lentiviral-mediated Smad4 shRNA confirmed a dominant role of Smad4 in HBR-induced cardiogenesis. Conclusions/Significance: The use of HBR may pave the way to novel combinatorial strategies of molecular and stem cell therapy based on fine tuning of targeted Smad transciption and signaling leading to a high-throughput of cardiogenesis without the needs of gene transfer technologies. © 2010 Maioli et al.
Scuderi N.,University of Rome La Sapienza |
Ceccarelli S.,University of Rome La Sapienza |
Onesti M.G.,University of Rome La Sapienza |
Fioramonti P.,University of Rome La Sapienza |
And 5 more authors.
Cell Transplantation | Year: 2013
The present study was designed to evaluate the clinical outcome of cell-based therapy with cultured adipose-derived stromal cells (ASCs) for the treatment of cutaneous manifestations in patients affected by systemic sclerosis (SSc). ASCs have an extraordinary developmental plasticity, including the ability to undergo multilineage differentiation and self-renewal. Moreover, ASCs can be easily harvested from small volumes of liposuction aspirate, showing great in vitro viability and proliferation rate. Here we isolated, characterized, and expanded ASCs, assessing both their mesenchymal origin and their capability to differentiate towards the adipogenic, osteogenic, and chondrogenic lineage. We developed an effective method for ASCs transplantation into sclerodermic patients by means of a hyaluronic acid (HA) solution, which allowed us to achieve precise structural modifications. ASCs were isolated from subcutaneous adipose tissue of six sclerodermic patients and cultured in a chemical-defined medium before autologous transplantation to restore skin sequelae. The results indicated that transplantation of a combination of ASCs in HA solution determined a significant improvement in tightening of the skin without complications such as anechoic areas, fat necrosis, or infections, thus suggesting that ASCs are a potentially valuable source of cells for skin therapy in rare diseases such as SSc and generally in skin disorders. © 2013 Cognizant Comm. Corp.