Lexington, MA, United States
Lexington, MA, United States

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Dickerson M.,Bioscale
Journal of visualized experiments : JoVE | Year: 2011

Custom assays for unique proteins are often limited to time consuming manual detection and quantitation techniques such as ELISA or Western blots due to the complexity of development on alternate platforms. BioScale's proprietary Acoustic Membrane MicroParticle (AMMP) technology allows sandwich immunoassays to be easily developed for use on the ViBE platform, providing better sensitivity, reproducibility, and automated operation. Provided as an example, this protocol outlines the procedure for developing a custom Chinese Hamster Ovary- Host Cell Protein (CHO-HCP) assay. The general principles outlined here can be followed for the development of a wide variety of immunoassays. An AMMP assay measures antigen concentration by measuring changes in oscillation frequency caused by the binding of microparticles to the sensor surface to calculate. It consists of four major components: (1) a cartridge that contains a functionalized eight sensor chip (2) antibody labeled magnetic microparticles, (3) hapten tagged antibody that binds to the surface of the functionalized chip (4) samples containing the antigen of interest. BioScale's biosensor is a resonant device that contains eight individual membranes with separate fluidic paths. The membranes change oscillation frequency in response to mass accumulating on the surface and this frequency change is used to quantitate the amount of added mass. To facilitate use in a wide variety of immunoassays the sensor is functionalized with an anti-hapten antibody. Assay specific antibodies are modified through the covalent conjugation of a hapten tag to one antibody and biotin to the other. The biotin label is used to bind the antibody to streptavidin coupled magnetic beads which, in combination with the hapten-tagged antibody, are used to capture the analyte in a sandwich. The complex binds to the chip through the anti-hapten/hapten interaction. At the end of each assay run the sensors are cleaned with a dilute acid enabling the sequential analysis of columns from a 96-well plate. Here, we present the method for developing a custom CHO-HCP AMMP assay for bioprocess development. Developing AMMP assays or modifying existing assays into AMMP assays can provide better performance (reproducibility, sensitivity) in complex samples and reduced operator time. The protocol shows the steps for development and the discussion section reviews representative results. For a more in-depth explanation of assay optimization and customization parameters contact BioScale. This kit offers generic bioprocess development assays such as Residual Protein A, Product titer, and CHO-HCP.


Patent
Bioscale | Date: 2013-09-16

In some aspects, an electrically responsive device can include a composite structure having spatially modulated structural properties that includes a substrate material having a surface and defining a plane; an electrically responsive material layer formed over at least a portion of the surface of the substrate material; an electrode material over portions of the electrically responsive material; and a stiffening material disposed along the electrode material, where the stiffening material has a thickness that varies and has regions of increased thickness that correspond with the regions of the composite structure along which the electrode material is disposed. The spatially modulated structural properties can include the regions of the composite structure along which the electrode material is disposed having the increased material stiffness, exclusive of the electrode material, relative to regions of the composite structure that do not include the electrode material.


In one non-limiting aspect, the invention provides a method for detecting the quality of a biological molecule comprising forming a first mixture of ingredients comprising: (i) a first binding agent that specifically binds to a tag, wherein the first binding agent is attached to a solid support; (ii) a decoy comprising a first portion comprising the tag attached to a second portion comprising an anchor; (iii) a sensor attached to a second binding agent that specifically binds to the anchor; and (iv) a sample suspected of containing a high quality biological molecule comprising a tag, wherein the tag of the high quality biological molecule is accessible; allowing interaction of the ingredients such that the sensor provides an output signal


Provided are methods and kits for analyzing biomarkers in one or more signal transduction pathways in a cell. In some embodiments, the methods and kits of the invention permit simultaneous analysis of more than one biomarker and/or more than one signal transduction pathway. In some embodiments, the invention provides methods for detecting whether a cell treated with an agent targeting a targeted biomarker is responding to the agent, or whether the cell is developing resistance to the agent. In some embodiments, the invention provides methods for determining which biomarker to target in a diseased or damaged cell, or which pathway an agent is targeting in an agent-treated cell. The invention provides kits for carrying out the described methods.


Trademark
Bioscale | Date: 2012-05-08

fluidic cartridge with microelectromechanical sensor(s).


Trademark
Bioscale | Date: 2012-04-24

system for detecting analytes comprised of bioanalyzer, housing, fluidic handling robotics, fluidic cartridge with microelectromechanical sensor(s), and computer software programs for use in capturing, detecting, quantitating and analyzing analytes.


Patent
Bioscale | Date: 2011-02-16

An apparatus for detecting a substance in a fluid is provided. A plurality of particles coated with a plurality of capture agents having an affinity for one or more biological markers is combined with bioprocess fluid to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to a sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.


Patent
Bioscale | Date: 2010-05-25

Methods and apparatus for analyzing bioprocess fluids are provided. A plurality of particles coated with a plurality of capture agents having an affinity for one or more biological markers is combined with bioprocess fluid to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to a sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.


Trademark
Bioscale | Date: 2016-02-23

reagents, assays, biomarkers and clinical targets for scientific or laboratory use. medical diagnostic reagents, assays, biomarkers and clinical targets for medical and clinical use; diagnostic kits comprised of reagents, assays, biomarkers and/or clinical targets that test for the presence of diseases, infections and pathogens and for patient management; diagnostic kits comprised of reagents, assays, biomarkers and/or clinical targets for capturing, detecting, quantitating, analyzing and processing analytes. system for detecting analytes and for fluidic handling comprised of bioanalyzer, housing, robotics, fluidic cartridge with microelectromechanical sensor/s (MEMS) and computer software for use in capturing, detecting, quantitating, analyzing and processing analytes; computer programs, downloadable computer programs and mobile device software for use in capturing, detecting, quantitating, analyzing and processing analytes; fluidic cartridge with microelectromechanical sensor(s); bio-molecular sensing systems and microelectronic mechanical systems comprised of functionalized biochips that permit capture of analytics for measurement, sensors comprised of microelectromechanical resonant structures for testing samples for use in food and water, testing, medical diagnostics, environmental monitoring, pharmaceuticals and therapeutics development and production as well as materials characterization; modular components for fluidic transport and delivery of samples to sensors, namely, fluid channels, valves, filters, concentrators, separators and pumps; electronics, namely, printed circuit boards for excitation and sensing of motion of the microstructures and microprocessor-based controllers for acquiring and processing sensor output and controlling system operation; electrical components, namely, connectors, cables, heaters, thermoelectric coolers and temperature sensors for interfacing biochips to systems and environmental controls; computer software for operating bio-molecular sensing systems and graphical user interface software all for use in the fields of biological, chemical and biochemical sensing; and computer programs, downloadable computer programs and mobile device software for use in processing analytes from diagnostic kits, reagents, assays, biomarkers and clinical targets, for use in patient stratification and for use in measuring biomarker levels. medical equipment in the nature of a bio-molecular sensing system and microelectronic mechanical system comprised of functionalized biochips that permit capture of analytics for measurement; medical equipment in the nature of micro-electromechanical system (MEMS) resonating structures and sensors for use in the analysis of physiological fluids, solids, aerosols and gases; medical equipment, namely, modular components for fluidic transport, and delivery of samples to sensors for use in point of care diagnosis as well as laboratory testing. Research and development services in the fields of reagent, assay, biomarker and clinical target development.


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