Lexington, MA, United States
Lexington, MA, United States
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Dong H.,Bristol Myers Squibb | Mora J.R.,Bristol Myers Squibb | Brockus C.,Bristol Myers Squibb | Chilewski S.D.,Bristol Myers Squibb | And 4 more authors.
AAPS Journal | Year: 2015

Immunogenicity testing for PEGylated biotherapeutics should include methods to detect both anti-protein and anti-PEG antibodies (anti-PEG). Although some methods have been published for the detection of anti-PEG antibodies, the information is incomplete and, in some cases, reagents used (such as Tween-20) are known to interfere with detection. This rapid communication describes the use of BioScale’s Acoustic Membrane MicroParticle (AMMP®) technology using the ViBE® Workstation to measure anti-PEG antibodies in human serum samples. Briefly, a sample spiked with monoclonal human IgG anti-PEG antibody is diluted in buffer and incubated with paramagnetic beads coated with linear chain mPEG to capture anti-PEG antibodies. The complex is then captured on an acoustic membrane coated with Protein A. The change in mass on the membrane caused by the binding of the complex to the membrane results in a signal proportional to the mass of anti-PEG antibodies. The data indicate that an assay with a sensitivity of less than 1000 ng/mL for IgG is achievable. This level of sensitivity is better than current published reports on IgG anti-PEG antibody detection. © 2015, American Association of Pharmaceutical Scientists.


Patent
Bioscale | Date: 2013-09-16

In some aspects, an electrically responsive device can include a composite structure having spatially modulated structural properties that includes a substrate material having a surface and defining a plane; an electrically responsive material layer formed over at least a portion of the surface of the substrate material; an electrode material over portions of the electrically responsive material; and a stiffening material disposed along the electrode material, where the stiffening material has a thickness that varies and has regions of increased thickness that correspond with the regions of the composite structure along which the electrode material is disposed. The spatially modulated structural properties can include the regions of the composite structure along which the electrode material is disposed having the increased material stiffness, exclusive of the electrode material, relative to regions of the composite structure that do not include the electrode material.


In one non-limiting aspect, the invention provides a method for detecting the quality of a biological molecule comprising forming a first mixture of ingredients comprising: (i) a first binding agent that specifically binds to a tag, wherein the first binding agent is attached to a solid support; (ii) a decoy comprising a first portion comprising the tag attached to a second portion comprising an anchor; (iii) a sensor attached to a second binding agent that specifically binds to the anchor; and (iv) a sample suspected of containing a high quality biological molecule comprising a tag, wherein the tag of the high quality biological molecule is accessible; allowing interaction of the ingredients such that the sensor provides an output signal


Provided are methods and kits for analyzing biomarkers in one or more signal transduction pathways in a cell. In some embodiments, the methods and kits of the invention permit simultaneous analysis of more than one biomarker and/or more than one signal transduction pathway. In some embodiments, the invention provides methods for detecting whether a cell treated with an agent targeting a targeted biomarker is responding to the agent, or whether the cell is developing resistance to the agent. In some embodiments, the invention provides methods for determining which biomarker to target in a diseased or damaged cell, or which pathway an agent is targeting in an agent-treated cell. The invention provides kits for carrying out the described methods.


Trademark
Bioscale | Date: 2012-05-08

fluidic cartridge with microelectromechanical sensor(s).


Trademark
Bioscale | Date: 2012-04-24

system for detecting analytes comprised of bioanalyzer, housing, fluidic handling robotics, fluidic cartridge with microelectromechanical sensor(s), and computer software programs for use in capturing, detecting, quantitating and analyzing analytes.


Patent
Bioscale | Date: 2011-02-16

An apparatus for detecting a substance in a fluid is provided. A plurality of particles coated with a plurality of capture agents having an affinity for one or more biological markers is combined with bioprocess fluid to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to a sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.


Patent
Bioscale | Date: 2010-05-25

Methods and apparatus for analyzing bioprocess fluids are provided. A plurality of particles coated with a plurality of capture agents having an affinity for one or more biological markers is combined with bioprocess fluid to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to a sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.


Trademark
Bioscale | Date: 2016-02-23

reagents, assays, biomarkers and clinical targets for scientific or laboratory use. medical diagnostic reagents, assays, biomarkers and clinical targets for medical and clinical use; diagnostic kits comprised of reagents, assays, biomarkers and/or clinical targets that test for the presence of diseases, infections and pathogens and for patient management; diagnostic kits comprised of reagents, assays, biomarkers and/or clinical targets for capturing, detecting, quantitating, analyzing and processing analytes. system for detecting analytes and for fluidic handling comprised of bioanalyzer, housing, robotics, fluidic cartridge with microelectromechanical sensor/s (MEMS) and computer software for use in capturing, detecting, quantitating, analyzing and processing analytes; computer programs, downloadable computer programs and mobile device software for use in capturing, detecting, quantitating, analyzing and processing analytes; fluidic cartridge with microelectromechanical sensor(s); bio-molecular sensing systems and microelectronic mechanical systems comprised of functionalized biochips that permit capture of analytics for measurement, sensors comprised of microelectromechanical resonant structures for testing samples for use in food and water, testing, medical diagnostics, environmental monitoring, pharmaceuticals and therapeutics development and production as well as materials characterization; modular components for fluidic transport and delivery of samples to sensors, namely, fluid channels, valves, filters, concentrators, separators and pumps; electronics, namely, printed circuit boards for excitation and sensing of motion of the microstructures and microprocessor-based controllers for acquiring and processing sensor output and controlling system operation; electrical components, namely, connectors, cables, heaters, thermoelectric coolers and temperature sensors for interfacing biochips to systems and environmental controls; computer software for operating bio-molecular sensing systems and graphical user interface software all for use in the fields of biological, chemical and biochemical sensing; and computer programs, downloadable computer programs and mobile device software for use in processing analytes from diagnostic kits, reagents, assays, biomarkers and clinical targets, for use in patient stratification and for use in measuring biomarker levels. medical equipment in the nature of a bio-molecular sensing system and microelectronic mechanical system comprised of functionalized biochips that permit capture of analytics for measurement; medical equipment in the nature of micro-electromechanical system (MEMS) resonating structures and sensors for use in the analysis of physiological fluids, solids, aerosols and gases; medical equipment, namely, modular components for fluidic transport, and delivery of samples to sensors for use in point of care diagnosis as well as laboratory testing. Research and development services in the fields of reagent, assay, biomarker and clinical target development.


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