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Mar del Plata, Argentina

Tkach A.D.,Bioquimica | Moreno J.D.,Bioquimico | Bava A.J.,National University of La Plata
Acta Bioquimica Clinica Latinoamericana | Year: 2012

A case of AIDS-associated histoplasmosis diagnosed by microscopy from a blood smear performed during a hematologic study was reported. The smear, fixed with methanol and stained with 10% Giemsa solution, revealed with a 100X objective, 2-4 μ-diameter yeast - like structures within the leukocytes, showing a typical staining in cap and a peripheral clear halo, characteristic of Histoplasma capsulatum. To confirm the mycological finding, the same smear was stained with the Grocott technique, showing brownish yeasts in the leucocytes. The patient, clinically deteriorated and with advanced immunological disorder (<50 T CD4+ lymphocytes/μL), died the next day after the diagnosis was made, in spite of the established treatments. This finding highlights the need to have operators trained in the recognition of microbiological structures of diagnostic importance in hematological smears in a Reference Center of Infectious Diseases, and even in the General Laboratory. The immediate diagnosis of this and other serious infections, as Malaria, enables the etiologic treatment and increases the possibilities of therapeutic success. Source


Lopardo H.A.,Dr. en Ciencias Bioquimicas | Borgnia D.,Bioquimica | Mastroianni A.,Tecnico Of Laboratorio
Acta Bioquimica Clinica Latinoamericana | Year: 2012

Two extendedly-used swab transport devices with Stuart medium using Streptococcus pyogenes, Haemophilus influenzae, Neisseria gonorrhoeae and Streptococcus pneumoniae reference strains, were compared. Copan Venturi Transystem® (Copan Italia Spa, Brescia, Italy) was significantly more effective than Eurotubo (Deltalab, Rubí, Barcelona, Spain), probably because the narrowing of the tube, just at the beginning of the semisolid column (Venturi system), would yield a better conservation of diffrerent organisms. © 2012 Federacion Bioquimica de la Provincia de Buenos Aires. Source


Zotta C.M.,Instituto Nacional Of Epidemiologia Dr Juan H Jara | Chinen I.,Instituto Nacional Of Enfermedades Infecciosas Inei | Lavayen S.,Instituto Nacional Of Epidemiologia Dr Juan H Jara | Cepeda M.,Enfermera | And 5 more authors.
Salud(i)Ciencia | Year: 2015

Hemolytic uremic syndrome (HUS) is endemic in Argentina, where it presents one of the highest rates worldwide. Shiga toxin-producing Escherichia coli (STEC) has been recognized as the main agent of post-enteric HUS. The aims of this study were to demonstrate STEC infection and to establish the carrier status in household contacts of HUS cases. As part of the surveillance of HUS, stool and serum samples of 257 household contacts of patients hospitalized in Mar del Plata and nearby localities were studied between 1996-2011. To establish STEC infection, three diagnostic criteria were used: 1) isolation and characterization of the pathogen; 2) detection of free fecal Stx (StxMF); 3) detection of anti-Stx antibodies in serum. Only the first criterion was used for household contacts. Asymptomatic carriers of STEC among the household contacts of 12/75 (16.0%) HUS cases were detected. From a total of 257 contacts, 26 (10.1%) showed evidence of STEC infection. In 3 out of 12 HUS cases, their household contacts carried an identical STEC strain: O145:NM (stx2a, eae, ehxA, pattern ARENMX01.0061), O157:H7 (stx2a/stx2c, eae, ehxA, pattern AREXHX01.0447), and O121:H19 (stx2a, eae, ehxA, pattern AREXKX01.0011). In only one HUS case, the STEC strains were different. Given the risks of this microorganism for public health, it is essential to maintain an active surveillance system of HUS cases and their family and institutional contacts. Also it is important to perform health education activities, including providing information on routes of transmission and prevention measures, to reduce the impact of STEC infections. Copyright © Sociedad Iberoamericana de Información Científica (SIIC), 2015. Source


Ponce De Leon P.,Bioquimica | Ponce De Leon P.,Laboratorio Of Parasitologia | Di Vita S.,Estudiante de Bioquimica | Di Vita S.,Laboratorio Of Parasitologia | And 4 more authors.
Acta Bioquimica Clinica Latinoamericana | Year: 2011

Erythrocyte aggregation affects microcirculation and its study is important in vascular diseases. It was communicated that Ascaris lumbricoides can capture erythrocyte sialic acid (SA) and alter the anionic charge on the red cell. The aim of this work was to study SA capture by A. lumbricoides larvae incubated in vivo with erythrocytes. Work was performed with concentrated larvae ([ALLC]) incubated in 4 tubes with phosphate buffer and antibiotics. Group O erythrocytes were added in two Tubes. The remaining were Controls. They were incubated at 37 °C (5% CO2) for 24 and 28 hours. The larvae were separated, collected, concentrated and counted microscopically (larvae/mL: [ALLC]1:1500-1700; [ALLC]3:1600-1800; [ALLC]2 and 4:200-400). Aggregation Inhibition by Polybrene (AIP) and Blue Alcian (BA) techniques were used. AIP showed a significant difference between Polybrene Titers diluted with physiological solution and with [ALLC]1 and 2, which were incubated with erythrocytes 24 and 48 hours respectively. There was no change in the Title of the corresponding Controls. BA performed in [ALLC]1 and [ALLC]3 determined CapSAC = 6.65% ±0.36 and SAC[CLAL]3 % = 0.67% ±0.36. The experience showed SA capture by larvae incubated in vivo with erythrocytes and it suggested that they have no intrinsic SA. SA kidnapping during the larval migration could be important in parasite-host interaction. Source


De Las Mercedes Romero M.,Bioquimica | Robles M.,Laboratorio Of Micobacteriologia | Prieto R.,Medico | Bava A.J.,Jefe de la Seccion Parasitologia | Bava A.J.,National University of La Plata
Acta Bioquimica Clinica Latinoamericana | Year: 2011

The eventual co-infection of P. jiroveci with bacterial, fungal and parasitologic respiratory pathogens in 50 respiratory secretions, obtained by bronchoalveolar lavage, was evaluated. The samples belonged to AIDS patients treated at the Respiratory Intensive Care Unit of Muñiz Hospital. They were consecutively processed between 20/11/08 and 1/7/10 in different laboratories of Muñiz Hospital, resulting all of them positive for the presence of Pneumocystis jiroveci by microscopy in this Laboratory. The results obtained revealed co-infection in 7 (14%) of the evaluated samples, these values being lower than those expected, probably on account of the impaired immunologic status of the patients evaluated. Staphylococcus aureus and Acinetobacter sp. were the bacterial co-pathogens more frequently isolated; while Cryptococcus neoformans was encountered as the unique fungal co-pathogen. Neither Mycobacterium tuberculosis nor other mycobacterial or parasites were identified in the evaluated samples. Empiric antibiotic treatment, the absence of studies to detect viral pathogens, and inadequate handling of the samples could have decreased the number of co-infections detected. The coinfection of P. jiroveci with other respiratory pathogens must be taken into account at the time of evaluating an adequate anti-infective treatment in AIDS patients with respiratory disease. Source

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