Bioptic Inc. | Date: 2017-02-21
A multi-channel bio-separation system configured to utilize a cartridge that has a individual, separate integrated reagent (i.e., a separation buffer) reservoir dedicated for each separation channel. The multiple channels may have different characteristics, such as different separation medium of different chemistries, different separation length, different channel sizes and internal coatings. In one embodiment, the cartridge does not include integrated detection optics. Not all channels need to be operative. One or more of the channels in the cartridge may be dummy channels that are not operative (e.g., not provided with a capillary tube). A capillary tube may be routed between the reservoir/electrode (anode) of one channel to an electrode (cathode) in another channel, thus allowing a longer length of capillary tube to be used to define a longer separation channel to improve resolution.
Lee S.H.,Inha University |
Yun H.G.,Inha University |
Lee M.-H.,Inha University |
Choi S.H.,Bioptic Co. |
Kim K.H.,Inha University
Optics Communications | Year: 2013
Single-longitudinal-mode (SLM) operation characteristics of an erbium-doped fiber (EDF) ring laser with an EDF saturable absorber have been measured and compared under two different-wavelength control-pump cases. The main EDF ring cavity was pumped with a conventional 977-nm-wavelength gain-pump laser diode, the EDF saturable absorber section attached to the main cavity via an optical circulator was pumped with a control-pump-laser beam of either 980-nm or 1531.9-nm wavelength to deliver the ring-laser output of 1541.4-nm wavelength. Our experimental results indicate that the control-pump-laser beam in the 1530-nm-wavelength region provides higher output power and wider wavelength range of the SLM operation of the ring laser than that in the 980-nm-wavelength region does. © 2013 Elsevier B.V.
Kerekgyarto M.,Debrecen University |
Kerekes T.,Debrecen University |
Tsai E.,BiOptic Inc. |
Amirkhanian V.D.,BiOptic Inc. |
Guttman A.,Debrecen University
Electrophoresis | Year: 2012
CGE is a well-established separation technique for the analysis of biologically important molecules such as nucleic acids. The inherent high resolving power, rapid analysis times, excellent detection sensitivity, and quantification capabilities makes this method favorable compared to conventional manual polyacrylamide and agarose slab gel electrophoresis techniques. In this paper we introduce a novel single-channel capillary gel electrophoresis system with LED-induced fluorescence detection also utilizing a compact pen-shaped capillary cartridge design for automatic analysis of samples from a 96-well plate. To evaluate the suitability of the system, 1000 genomic DNA(gDNA) samples were analyzed in gel filled capillaries and detected by the microball ended excitation and emission optical fiber based LED-induced fluorescence detection system. Excellent migration time reproducibility of RSD <0.75% was obtained over the course of 1000 runs. The system rapidly distinguished between intact and degraded gDNA samples, therefore provided important information if they could be used for downstream quantitative PCR processing where high-quality intact gDNA was key. We envision that this novel system design will rapidly find new applications in both research and clinical diagnostic laboratories as a highly sensitive and easy to use bio-analytical approach. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Tsai S.-K.,Bioptic Inc. |
Amirkhanian V.D.,Bioptic Inc.
American Laboratory | Year: 2013
A single-channel CE instrument was developed for fluorescent immunoassay separation and detection that can be used in both research and clinical diagnostics laboratories. Affinity capillary electrophoresis (ACE) was applied to analyze antigen-antibody interactions by using polyclonal antibodies against crocalbin, a calcium -binding protein with EF-hand motif, as a model system. Using fluorescein isothiocyanate (FITC)-labeled protein sample, a sensitivity limit of 15 nM or less was attained for the capillary electrophoresis immunoassay platform. The I-CE instrument design is based on real-time fluorescence detection. It features an economical, disposable, pen-shaped gel cartridge for microseparations and fluorescent-labeled antibody fragment detection. The fully automated I-CE instrument includes an in-house-designed, automated, modular X-YZ mechanism for the buffer and sample tray. The compact I-CE design platform is able to rapidly resolve the reaction mixtures of antibody and antigen in a free-solution capillary electrophoresis system.
Amirkhanian V.,BiOptic Inc. |
Tsai S.-K.,BiOptic Inc.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2014
We introduce a novel and cost-effective capillary gel electrophoresis (CGE) system utilizing disposable pen-shaped gelcartridges for highly efficient, high speed, high throughput fluorescence detection of bio-molecules. The CGE system has been integrated with dual excitation and emission optical-fibers with micro-ball end design for fluorescence detection of bio-molecules separated and detected in a disposable pen-shaped capillary gel electrophoresis cartridge. The high-performance capillary gel electrophoresis (CGE) analyzer has been optimized for glycoprotein analysis type applications. Using commercially available labeling agent such as ANTS (8-aminonapthalene-1,3,6- trisulfonate) as an indicator, the capillary gel electrophoresis-based glycan analyzer provides high detection sensitivity and high resolving power in 2-5 minutes of separations. The system can hold total of 96 samples, which can be automatically analyzed within 4-5 hours. This affordable fiber optic based fluorescence detection system provides fast run times (4 minutes vs. 20 minutes with other CE systems), provides improved peak resolution, good linear dynamic range and reproducible migration times, that can be used in laboratories for high speed glycan (N-glycan) profiling applications. The CGE-based glycan analyzer will significantly increase the pace at which glycoprotein research is performed in the labs, saving hours of preparation time and assuring accurate, consistent and economical results. © 2014 SPIE.
Tsai E.,BiOptic Inc. |
Chi A.,BiOptic Inc. |
Yu M.,BiOptic Inc. |
Huang H.,BiOptic Inc. |
Amirkhanian V.,BiOptic Inc.
American Laboratory | Year: 2014
The Qsep1 portable CGE instrument from BiOptic is cost effective and operates in wireless mode using WiFi. The present iteration of the instrument provides simplified, high efficiency, highly sensitive results, is cost-effective and uses WiFi type communication for biomolecule detection applications. The portable CGE-based bioseparation system is configured to conduct bioseparations in a glass capillary separation channel (microfluidic) using a Smartphone app to operate via WiFi. In the portable CGE system, the fluorescence emission signals produced by the separated analytes are collected at the detection zone of the capillary using an optical fiber, which is then relayed to the detector module with a built-in emission filter for FITC, EtBr, or other related fluorescence detection applications. The portable CGE system accepts a single channel, pen-shaped gel cartridge that has an integrated top gel/buffer reservoir. The instrument is directly coupled to an external air pressure source.
Bioptic Inc. | Date: 2011-08-18
A cartridge-based bio-separation system configured to utilize a pen shaped bio-separation cartridge that is easy to assemble and use with no moving parts and that has an integrated reagent (separation buffer) reservoir. The cartridge includes a body, defining an opening as a detection window for receiving external detection optics, at least one capillary column supported in the body, having a first end extending beyond a first end of the body, wherein the detection window exposes a section along the capillary column, to which the external optics are aligned through the detection window, and a reservoir attached to a second end of the body in fluid flow communication with a second end of the capillary column. The reservoir is structured to be coupled to an air pressure pump that pressurizes the gel reservoir to purge and fill the capillaries with buffer as the separation support medium.
Bioptic Inc. | Date: 2014-06-24
A simple, low cost, efficient and stable micro-vial configuration for handling micro-volume of sample fluids. The interior wall geometry of the inventive vial is designed to include several axial sections of various interior diameters to provide a range of functionalities to address various design considerations. The interior wall defined in the vial has a cylindrical sample section, a wider cylindrical alignment section, a tapered or conical guide section, and a relatively large cylindrical body section, arranged in sequence in that order along the center axis of the vial. The sample section is designed to hold a small volume of a sample fluid, and to receive the tip end of a capillary tube. The alignment section has a larger diameter than the sample section, designed to receive a cylindrical support that coaxially supports the relatively fragile capillary tube. The tip of the capillary tube dips into the micro-volume of sample fluid held in the sample section. The conical section functions to guide the capillary tube and the support tube into the alignment section and the tip of the capillary tube into the sample section. The body section has the largest diameter, for holding additional fluid if desired.
Bioptic Inc. | Date: 2015-05-22
A method for glycan profiling by capillary electrophoresis (CE), and a CE system for glycan analysis (N-Glycan). The CE system uses integrated dual optical fibers for both radiation excitation and emission detection. The CE system is configured for performing a two-color detection for data analysis. A single radiation excitation source is used to excite two emission fluorophores or dyes in the sample solution to be analyzed. One emission dye is to tag the sample and the other dye is used to provide a reference marker (e.g., a Dextran Ladder) for the sample run. Two detectors (e.g., photomultipler tubes (PMTs)) are applied to simultaneously detect the fluorescent emissions from the dyes. The data collected by both detectors are correlated (e.g., synchronized, and/or super-positioned for analysis) for accurate data peak identification.
Bioptic Inc. | Date: 2011-01-28
A detection optics configuration for bio-analysis, in which the direction of incident radiation, the axis of the separation channel, and the direction of collection of the output radiation are coplanar at the detection zone. The detection configuration incorporates ball-end optical fibers to direct incident radiation at and collection of output radiation from the detection zone. The detection optics configuration may be implemented in an improved bio-separation instrument, in particular a capillary electrophoresis instrument.