Bioprocess Control Sweden AB

Lund, Sweden

Bioprocess Control Sweden AB

Lund, Sweden
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Shen S.,Lund University | Shen S.,Zhejiang University of Technology | Nges I.A.,Lund University | Yun J.,Zhejiang University of Technology | And 2 more authors.
Chemical Engineering Journal | Year: 2014

Various pre-treatments (acid, alkaline, enzyme and alkaline aided enzyme also termed combined) were evaluated on different fractions of bamboo waste from a chopstick production factory. Chemical oxygen demand (COD) solubilisation, monomeric/dimeric sugar yield, methane yield enhancement and methane production rate were assessed. The biochemical methane potential was determined in batch assays under mesophilic conditions (37. ±. 1. °C) using the Automatic Methane Potential Test System (AMPTS-II).Pre-treatments led to enhanced COD solubilisation as compared to raw sample. Alkaline aided enzymatic pre-treatment led to the highest sugar yield, comparable to the theoretical yield. However, high sugar yield did not translate to high methane yield. The best pre-treatment in terms of methane yield was alkaline pre-treatment which resulted in a surplus of up to 88% methane yield. There was a positive correlation between dissolved COD and methane yield. Methane yield and methane production rate also increased with decreasing particle sizes. In all investigated scenarios, pre-treatment led to an improved methane production rate as compared to the raw samples. These results demonstrated that alkaline pre-treatment at ambient temperature was an efficient treatment option to improve methane yield of bamboo waste. © 2013 Elsevier B.V.


Stromberg S.,Lund University | Possfelt M.O.,Lund University | Liu J.,Lund University | Liu J.,Bioprocess Control Sweden AB
Water Science and Technology | Year: 2013

Three previously published control strategies for anaerobic digestion were implemented in Simulink/Matlab using Anaerobic Digestion Model No. 1 (ADM1) to model the biological process. The controllers' performance were then simulated and evaluated based on their responses from five different types of process scenarios i.e. start-up and steady state performance as well as disturbances from concentration, pH and ammonia in the inflow. Of the three evaluated control strategies, the extremum-seeking variable gain controller gave the best overall performance. However, a proportional feedback controller based on the pH-level, used as a reference case in the evaluation, proved to give as good results as the extremum-seeking variable gain controller but with a lower wear on the pump. It was therefore concluded that a fast proportional control of the reactor pH is a key element for optimally controlling a low-buffering anaerobic digestion process. © IWA Publishing 2013.


Wang B.,Lund University | Stromberg S.,Lund University | Nges I.A.,Lund University | Nistor M.,Bioprocess Control Sweden AB | And 2 more authors.
Journal of Bioscience and Bioengineering | Year: 2016

Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37°C or storage at 4°C introduced a lag-time whereas the effects on hydrolysis rate were less consequent. In summary, pre-treatments affected the enzyme activity of the inoculum, and further on, significantly influenced the methane production and the degradation kinetics of the investigated substrates. It is recommended that filtration of inoculum should be avoided unless in case too large particles therein. © 2015 The Society for Biotechnology, Japan.


Patent
Bioprocess Control Sweden AB | Date: 2010-04-01

A system setup is disclosed for carrying out a biological methane potential test on a biosample and a system setup is disclosed for measuring biomethane gas in a biogas flow. In at least one embodiment, the system setups include at least one container for chemical irreversible fixation of other gases than biomethane gas, the other gases including at least CO_(2), and at least one gas flow measuring device.


Patent
Bioprocess Control Sweden Ab | Date: 2010-04-01

A measuring device is disclosed for measuring an ultra low gas flow, working by the principle of liquid displacement. In at least one embodiment, the measuring device includes at least one cell including a gas inflow device, a gas compartment device with a predefined inner geometric physical volume and active volume. In at least one embodiment, the gas compartment device includes one gas accumulating end and one lifting end, the gas compartment device also defining a geometric gas collecting point inside of the gas compartment device, during a gas filling cycle, the geometric gas collecting point moving further and further from the gas accumulating end to the lifting end during the gas filling cycle. Further, in at least one embodiment the cell includes a holding device having a pivoting element enabling the gas compartment device to pivot upwards when the geometric gas collecting point is positioned at the lifting end and the lifting force is larger than the down-pressing force at the lifting end, thereby releasing all of the accumulated gas in the gas compartment device, and then pivoting back to its initial standby position for new receipt and storage of gas during another gas filling cycle until next releasing sequence. Finally, in at least one embodiment, the cell also includes a sensor provided to generate a signal and/or change the state of a signal when the gas compartment device is not in its initial standby position, wherein the gas storing capacity of the inside of the gas compartment device is larger at the gas accumulating end than at the lifting end and wherein the gas accumulating end has a higher vertical position than the lifting end at the initial standby position.


Wang B.,Lund University | Stromberg S.,Lund University | Li C.,Lund University | Nges I.A.,Lund University | And 4 more authors.
Bioresource Technology | Year: 2015

In this study, two experiments were conducted to evaluate the impact of substrate concentrations on methane potential and degradation kinetics of substrate. The biochemical methane potential (BMP) tests in Experiment I were performed at a constant inoculum to substrate ratio (ISR), whereas, different ISRs were applied in Experiment II. Results obtained from Experiment I revealed that methane potential of substrate increased at a saturating trend with higher substrate concentrations, and could differ by up to 30% between the lowest and highest investigated concentrations. The results of Experiment II verified the results of Experiment I, and further showed that this trend also occurs when the substrate concentration is regulated with ISRs. In contrast, substrate concentration had no significant impact on the degradation kinetics. It was concluded that dilutions should be avoided when the substrate concentration is lower than 10. g. VS/L in order to avoid underestimations of methane potential from BMP test. © 2015 Elsevier Ltd.


Patent
Bioprocess Control Sweden Ab | Date: 2013-11-01

The present invention describes a system setup for monitoring and/or controlling one or multiple fermentation processes, said system setup comprisingat least one fermentation unit; a data acquisition unit; and a cloud computing unit having a database, a file storage capability, a data calculation capability and a user interface capability; wherein the at least one fermentation unit is connected to the acquisition unit which in turn is connected to the cloud computing unit so that on-line, real-time data on the one or multiple fermentation processes may be transferred from the at least one fermentation unit via the data acquisition unit to the cloud computing unit to be interpreted and displayed for a user being on-line, and wherein the system set-up enables measuring in the at least one fermentation unit and/or the data acquisition unit of the on-line, real-time data on the one or multiple fermentation processes, wherein the system setup also comprisesone or multiple laboratory simulation platform(s) and/or full-scale process(es) comprising said at least one fermentation unit, being in data connection with the cloud computing unit; and wherein the data acquisition unit is connected to the cloud computing unit so that all data acquisition, data interpretation and data storage is performed centralized on the cloud computing unit.


PubMed | Lund University and Bioprocess Control Sweden AB
Type: Journal Article | Journal: Journal of bioscience and bioengineering | Year: 2016

Biochemical methane potential (BMP) tests were carried out to investigate the influence of inoculum pre-treatments (filtration and pre-incubation) on methane production from cellulose and wheat straw. First-order model and Monod model were used to evaluate the kinetic constants of the BMP assays. The results demonstrated that fresh inoculum was the best option to perform BMP tests. This was evidenced by highest enzyme activity (0.11 U/mL) and highest methane yields for cellulose (356 NmL CH4/gVS) as well as wheat straw (261 NmL CH4/gVS). Besides, high biodegradability (85.8% for cellulose and 61.3% for wheat straw) was also obtained when the fresh inoculum was used. Moreover, a kinetic evaluation showed that inoculum pre-incubation at 37C or storage at 4C introduced a lag-time whereas the effects on hydrolysis rate were less consequent. In summary, pre-treatments affected the enzyme activity of the inoculum, and further on, significantly influenced the methane production and the degradation kinetics of the investigated substrates. It is recommended that filtration of inoculum should be avoided unless in case too large particles therein.


PubMed | Lund University and Bioprocess Control Sweden AB
Type: Journal Article | Journal: Water science and technology : a journal of the International Association on Water Pollution Research | Year: 2014

In this work, biochemical methane potential (BMP) tests with cellulose as a model substrate were performed with the aid of three manually operated or conventional experimental setups (based on manometer, water column and gas bag) and one automated apparatus specially designed for analysis of BMP. The methane yields were 340 18, 354 13, 345 15 and 366 5 ml CH4/g VS obtained from experimental setups with manometer, water column, gas bag, and automatic methane potential test system, which corresponded to a biodegradability of 82, 85, 83 and 88% respectively. The results demonstrated that the methane yields of cellulose obtained from conventional and automatic experimental setups were comparable; however, the methane yield obtained from the automated apparatus showed greater precision. Moreover, conventional setups for the BMP test were more time- and labour-intensive compared with the automated apparatus.


PubMed | Biogas Institute of Ministry of Agriculture, Lund University and Bioprocess Control Sweden AB
Type: | Journal: Bioresource technology | Year: 2015

In this study, two experiments were conducted to evaluate the impact of substrate concentrations on methane potential and degradation kinetics of substrate. The biochemical methane potential (BMP) tests in Experiment I were performed at a constant inoculum to substrate ratio (ISR), whereas, different ISRs were applied in Experiment II. Results obtained from Experiment I revealed that methane potential of substrate increased at a saturating trend with higher substrate concentrations, and could differ by up to 30% between the lowest and highest investigated concentrations. The results of Experiment II verified the results of Experiment I, and further showed that this trend also occurs when the substrate concentration is regulated with ISRs. In contrast, substrate concentration had no significant impact on the degradation kinetics. It was concluded that dilutions should be avoided when the substrate concentration is lower than 10 g VS/L in order to avoid underestimations of methane potential from BMP test.

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