Bioprocess and Molecular Engineering Research Unit

Kuala Lumpur, Malaysia

Bioprocess and Molecular Engineering Research Unit

Kuala Lumpur, Malaysia
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Hassan N.A.,Bioprocess and Molecular Engineering Research Unit | Amid A.,Bioprocess and Molecular Engineering Research Unit | Mohd Salleh H.,Bioprocess and Molecular Engineering Research Unit | Mohd Salleh H.,International Islamic University Malaysia
Journal of Pure and Applied Microbiology | Year: 2013

VDAC2 protein identified to be overexpressed in stunned chicken is a potential biomarker in differentiating stunned chicken since the expression was proportional to the voltage applied. To facilitate research, the gene encoding VDAC is cloned, expressed and optimized in BL21-AI Escherichia coli. CDNA was synthesized from mRNA of stunned chicken's skeletal muscle and cloned using recombination reaction. All positive clones were identified by PCR and restriction enzyme digestion as well as DNA sequencing. Recombinant VDAC2 protein was purified by Ni-NTA spin-column. Western blot performed using polyclonal anti-N terminal human VDAC2 and anti-His tag revealed a 34 kDa protein, confirming the expression of recombinant VDAC2.


Fouz N.,Bioprocess and Molecular Engineering Research Unit | Amid A.,Bioprocess and Molecular Engineering Research Unit | Amid A.,International Islamic University Malaysia | Hashim Y.Z.H.-Y.,Bioprocess and Molecular Engineering Research Unit | Hashim Y.Z.H.-Y.,Bioenvironmental Research Center
Asian Pacific Journal of Cancer Prevention | Year: 2013

Background: Breast cancer is a leading cause of death in women. The available chemotherapy drugs have been associated with many side effects. Bromelain has novel medicinal qualities including anti-inflammatory, anti-thrombotic, fibrinolytic and anti-cancer functions. Commercially available bromelain is obtained through tedious methods; therefore, recombinant bromelain may provide a cheaper and simpler choice with similar quality. Materials and Methods: This study aimed to assess the effects of commercial and recombinant bromelain on the cytokinetic behavior of MCF-7 breast cancer cells and their potential as therapeutic alternatives in cancer treatment. Cytotoxic activities of commercial and recombinant bromelain were determined using (sulforhodamine) SRB assay. Next, cell viability assays were conducted to determine effects of commercial and recombinant bromelain on MCF-7 cell cytokinetic behavior. Finally, the established growth kinetic data were used to modify a model that predicts the effects of commercial and recombinant bromelain on MCF-7 cells. Results: Commercial and recombinant bromelain exerted strong effects towards decreasing the cell viability of MCF-7 cells with IC50 values of 5.13 μg/mL and 6.25 μg/mL, respectively, compared to taxol with an IC50 value of 0.063 μg/mL. The present results indicate that commercial and recombinant bromelain both have anti-proliferative activity, reduced the number of cell generations from 3.92 to 2.81 for commercial bromelain and to 2.86 for recombinant bromelain, while with taxol reduction was to 3.12. Microscopic observation of bromelain-treated MCF-7 cells demonstrated detachment. Inhibition activity was verified with growth rates decreased dynamically from 0.009 h-1 to 0.0059 h-1 for commercial bromelain and to 0.0063 h-1 for recombinant bromelain. Conclusions: Commercial and recombinant bromelain both affect cytokinetics of MCF-7 cells by decreasing cell viability, demonstrating similar strength to taxol.


Yusilawati A.N.,Bioprocess and Molecular Engineering Research Unit | Maizirwan M.,Bioprocess and Molecular Engineering Research Unit | Sopyan I.,Biomedical Engineering Research Group | Hamzah M.S.,Halal Industry Research Center | And 2 more authors.
Advanced Materials Research | Year: 2011

It is known that polystyrene must be chemically modified to make its surface amenable to covalent cross-linking with protein. The aim of this study was to set up a UV/Ozone system and investigate the effects of UV/Ozone treatment on polystyrene surface. Microsize polystyrene beads with an average size of 150 μm in diameter were treated with and without distilled water at the same treatment time, ozone flow-rate and UV intensity. The treated beads were analyzed by ATRFTIR, SEM, EDX and hydrophilicity measurement. The results show that the hydrophilicity of the surface of polystyrene beads was increased after the UV/ozone treatment and the introduction of carbonyl, carboxyl and hydroxyl groups on the polystyrene beads surface was also confirmed. It was demonstrated that the UV/Ozone system was effective for treatment of polystyrene bead and the best result was obtained without distilled water. © (2011) Trans Tech Publications, Switzerland.

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