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Tralhao J.G.,University of Sfax | Abrantes A.M.,Biophysics Biomathematics Institute | Hoti E.,Saint Vincents University Hospital | Figueirinha R.,Biophysics Biomathematics Institute | And 2 more authors.
Hepato-Gastroenterology | Year: 2012

Background/Aims: This prospective study aimed to estimate the usefulness of the hepatic extraction fraction (HEF) in the context of the pre- and postoperative evaluation of patients with hepatic tumours. Methodology: Seventy patients with colorectal metastases (n=25), hepatocellular carcinoma (n=25), cholangiocellular carcinoma (n=6), gastric cancer metastases (n=5), hemangioma (n=5) and others (n=4) were included. Thirty patients underwent hepatectomy. Child-Pugh score, prothrombin, albumin, ALT, AST, AF, LDH, total, direct and indirect bilirubin, platelet number as well as the HEF were evaluated in the preoperative period and one month after hepatectomy. Results: Preoperative evaluation of HEF values between Child-Pugh A (93.6±17.3%) and Child-Pugh B/C (n=13; 58.1±28.6%) demonstrated significant differences (p=0.001). We found a high negative correlation between the preoperative HEF and ALT (p<0.001), AST (p<0.001), AF (j<0.001), TB (p<0.001), IB (p<0.001) and DB (j<0.001), and also a high positive correlation between the preoperative HEF and albumin (p<0.001) or prothrombin (p<0.01). All operated patients had a normal HEF and a positive correlation between the postoperative HEF and albumin (p<0.05) at one month after surgery. Conclusions: The HEF allows a dynamic evaluation of hepatocellular function, which is not possible with other clinical, biological and radiological methods. © H.G.E. Update Medical Publishing S.A.

Lopes S.M.M.,University of Coimbra | Laranjo M.,Biophysics Biomathematics Institute | Serra A.C.,University of Coimbra | Abrantes A.M.,Biophysics Biomathematics Institute | And 5 more authors.
Journal of Heterocyclic Chemistry | Year: 2010

(Chemical Equation Presented) Naphthoquinones undergo 1,3-dipolar cycloaddition with bicyclic münchnones generated from thiazolidines affording new pyrrolo-thiazoles with a fused quinone nucleus. The products were obtained as single enantiomers in good yields. These benzo[f]thiazolo[4,3-a] isoindole-6,11(1H,3H)-diones are comprised of four fused rings and present a very planar structure. The evaluation of their anticancer activity against melanoma A375 and colorectal adenocarcinoma WiDr human cell lines showed only moderate activity but gave insight into the modeling of new structures. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.] © 2010 HeteroCorporation.

Tralhao J.G.,University of Sfax | Tralhao J.G.,Biophysics Biomathematics Institute | Hoti E.,Saint Vincents University Hospital | Oliveiros B.,Biophysics Biomathematics Institute | And 2 more authors.
Hepato-Gastroenterology | Year: 2012

Background/Aims: To investigate the impact of liver resection on the perioperative hepatic function by evaluation of ICG-clearance. Methodology: Twenty-five patients underwent major hepatic resection (Group A) and 36 underwent minor hepatic resection (Group B). Thirteen patients who received no liver surgery, acted as control group (Group C). ICG-clearance measured by a non-invasive technique was expressed in terms of plasma disappearance rate (PDR-ICG-%/min) and retention rate of ICG 15 min (ICG-R15-%) after administration ICG (0.5mg/kg) before anesthesia induction (T0), immediately after the surgery (T1) and 24h after (T2). Results: There was statistically significant differences between the three groups: ICG-PDR (p<0.004) ICG-R15 (p<0.040). These differences were observed between groups A and C at T1, between A and B, and C, at T2 for ICG-PDR and between groups A and T2 for ICG-R15. There were no differences between groups for hemoglobin, platelets, PT, creatinine, albumin total protein, bilirubin and ALP. Conclusions: ICG clearance is a safe non-invasive dynamic tool to quantify the liver function in patients following hepatic surgery. It also can be used to evaluate the liver surgery impact on hepatic function which can help to diagnose early hepatic dysfunction and guide the therapeutic decision making process. © H.G.E. Update Medical Publishing S.A.

Abrantes A.M.,Biophysics Biomathematics Institute | Abrantes A.M.,University of Coimbra | Serra M.E.S.,University of Coimbra | Goncalves A.C.,University of Coimbra | And 7 more authors.
Nuclear Medicine and Biology | Year: 2010

Colorectal cancer is one of the most common malignancies in the Western world and is an example of a solid tumour in which hypoxia is a common feature and develops because of the inability of the vascular system to supply adequate amounts of oxygen to growing tumours. Hypoxia effects on tumour cell biology can be detected and characterized using different methods. The use of imaging with γ-emitting radionuclides to detect hypoxic tissue was first suggested by Chapman in 1979 [N Engl J Med 301 (1979) 1429-1432]. 99mTc-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime, also known as 99mTc-HL-91, has been among the most studied hypoxia markers. The objective of this study was to correlate the uptake of 99mTc-HL-91 and 99mTc-MIBI in colon cancer cells under normoxic and hypoxic conditions and to compare this information with some parameters such as oxidative stress and mitochondrial dysfunction of the cells analyzed by flow cytometry. Our results show that the in vitro 99mTc-HL-91 uptake is higher in hypoxic conditions, which is confirmed by the decreased uptake of 99mTc-MIBI. Flow cytometry results demonstrate that hypoxic conditions used are not enough to induce cellular death, but are responsible for the alterations in the intracellular redox environment, namely, increase of ROS production, proteic pimonidazol-derived adduct formation and alteration in the mitochondrial membrane permeability. Therefore, these results confirm that 99mTc-HL-91 is a radiopharmaceutical with favourable characteristics for detecting hypoxia. © 2010 Elsevier Inc. All rights reserved.

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