Freudiger C.W.,Harvard University |
Min W.,Harvard University |
Holtom G.R.,Harvard University |
Xu B.,Biophotonic Solutions Inc. |
And 2 more authors.
Nature Photonics | Year: 2011
Label-free microscopy that has chemical contrast and high acquisition speeds up to video rates has recently been made possible using stimulated Raman scattering (SRS) microscopy. SRS imaging offers high sensitivity, but the spectral specificity of the original narrowband implementation is limited, making it difficult to distinguish chemical species with overlapping Raman bands. Here, we present a highly specific imaging method that allows mapping of a particular chemical species in the presence of interfering species, based on tailored multiplex excitation of its vibrational spectrum. This is implemented by spectral modulation of a broadband pump beam at a high frequency (>1 MHz), allowing detection of the SRS signal of the narrowband Stokes beam with high sensitivity. Using the scheme, we demonstrate quantification of cholesterol in the presence of lipids, and real-time three-dimensional spectral imaging of protein, stearic acid and oleic acid in live Caenorhabditis elegans. © 2011 Macmillan Publishers Limited. All rights reserved.
Coello Y.,Michigan State University |
Daniel Jones A.,Michigan State University |
Gunaratne T.C.,Biophotonic Solutions Inc. |
Dantus M.,Michigan State University
Analytical Chemistry | Year: 2010
A novel atmospheric pressure imaging mass spectrometry approach that offers improved lateral resolution (10 μm) using near-infrared femtosecond laser pulses for non-resonant desorption and ionization of sample constituents without the need of a laser-absorbing matrix is demonstrated. As a proof of concept the method was used to image a two-chemical pattern in paper. To demonstrate the ability of the approach to analyze biological tissue, a monolayer of onion epidermis was imaged allowing the chemical visualization of individual cells using mass spectrometry at ambient conditions for the first time. As the spatial resolution is currently limited by the limit of detection of the setup (∼500 fmol limit of detection for citric acid), improvements in sensitivity will increase the achievable spatial resolution. © 2010 American Chemical Society.
Agency: Department of Defense | Branch: Army | Program: STTR | Phase: Phase II | Award Amount: 750.00K | Year: 2005
The desired outcome of this STTR project is the development of compact, stand-alone, economical, and rugged pulse shaper units that can be used to control chemical reactions, and have a wide range of applications based on molecular control for detection of chemical and biological threats, explosive, and toxic chemicals. In addition, these units will enable a broad range of fundamental studies. During Phase II we will subject the breadboard unit successfully developed in Phase I to rigorous testing and evaluation by experts in order to develop an alpha version that will undergo further testing resulting in the beta version pre-commercial prototype. The beta version prototype will be ready for field evaluation by a number of customers in the research, industrial, and military markets, and will be operable for longer than a week without maintenance. Phase III will focus on sales of the commercial products via strategic partnership with laser manufacturers and end users. Based on technical attributes and on a strong intellectual property position, Biophotonic Solutions is poised to become the leader in pulse shaping equipment and its applications in the Homeland Security, Biomedical Technology and research markets.
Agency: Department of Defense | Branch: Army | Program: STTR | Phase: Phase I | Award Amount: 99.86K | Year: 2004
Monitoring the air for potential chemical and biological agents (from terrorist threats or from industrial contamination) has become a necessity. Our proposal objective is to develop device capable of fast (1 second), accurate (even in a chemically complex environment), robust (stand alone, closed-loop, and portable), and reproducible sensing. Operationally, the device interfaces with a commercially available femtosecond pulsed laser and mass spectrometry module. The device operates on the principle of molecular control based on shaped laser fields. Using genetic-algorithm (GA) search methods, a series of laser fields are determined to unequivocally identify each chemical or biological agent of interest. The stand-alone unit monitors for suspected chemical agents. Multiple electromagnetic fields increase the accuracy of chemical identification a million-fold compared to available sensor methods. Upon positive identification, the unit contacts a command center. The goal, for phase I will be to demonstrate that differently shaped laser fields produce uniquely different fragmentation mass spectra, and that such fields can be determined for each chemical or biological sample. Phase II of the project will concentrate on the development of a field-ready stand-alone module capable of detection of contaminants at part per billion levels even in the presence of a chemically complex environment.
News Article | October 1, 2013
Biophotonic Solutions Inc., an East Lansing, MI-based provider of automated laser pulse compression solutions, closed a $1m Series A funding. The round was led by the Michigan Angel Fund. Led by Kiyomi Monro, CEO, BSI develops ultrafast lasers with adaptive capabilities producing pulses in the multi-femtosecond range used for industrial, scientific, medical, and defense applications. The company’s products are based on proprietary technology, called “MIIPS”, which provides automated measurement and compression of ultrafast laser pulses, automatically delivering optimized laser light to the target.