Biopharmaceutical R and nter

Taizhou, China

Biopharmaceutical R and nter

Taizhou, China
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Li Y.-M.,Biopharmaceutical R and nter | Huang Y.-D.,Biopharmaceutical R and nter | Xiang Q.,Biopharmaceutical R and nter | Qu H.-Y.,Biopharmaceutical R and nter | And 2 more authors.
Journal of China Pharmaceutical University | Year: 2010

Aim: To investigate a new type wound dressing, basic fibroblast growth factor(bFGF)/collagen composite sponge, and conduct its pharmacological studies in vitro and in vivo. Methods: bFGF/collagen composite sponge was prepared using fresh pig skin and bFGF. The sponge's physicochemical properties were studied. MTT assay was used to detect the proliferation effect of the sponge extract on 3T3 cells. Delayed allergy of the sponge was tested for the assurance of its biosafety. Results: Results showed that the physicochemical properties of bFGF/collagen composite sponge with high and low doses of bFGF have no significant difference from those of blank collagen sponge. SDS-page analysis indicated that the composite sponge has apparent strip in 18 kD. It was also found that bFGF/collagen composite sponge was responsible for significant effects on 3T3 cell proliferation in comparison to saline treatement (P <0.01, P <0.05). In the allergy study, during the periods of the induction and stimulation, no allergic reaction was found in bFGF/collagen composite sponge groups with high and low doses of bFGF, while severe reactions and inflammation occurred in positive group (2,4-dinitrochlorobenzene). Furthermore, pathological examination indicated the intact dermal structure and no sign of inflammation. Conclusion: The developed sponge has good physicochemical propertis and noticed cellular proliferation without dermal irritation. There is much potential to develop bFGF/collagen composite sponge into a new kind of wound dressing material for clinical use.


Jiang J.,Zhejiang University of Technology | Jiang J.,Biopharmaceutical R and nter
Asian Journal of Chemistry | Year: 2015

Seven novel 1,3,5-triazine derivatives were synthesized in good to high yields (69.19-91.15%) by the reaction of substituted guanidine with 2-(2-hydroxyphenyl)-4H-benzo[e][1,3]oxazin-4-one in ethanol. The products were recrystallized from ethanol or ethanol-DMF mixture and their structures were confirmed by 1H NMR and FT-IR.


Zhang S.,Biopharmaceutical R and nter | Ke Z.,Biopharmaceutical R and nter | Qian S.,Biopharmaceutical R and nter | Xie D.,Biopharmaceutical R and nter | And 4 more authors.
Lizi Jiaohuan Yu Xifu/Ion Exchange and Adsorption | Year: 2012

The influencing factors of nitration of o-xylene with strong acid ion exchange resin and 95% nitric acid as nitrating agent were investigated by single factor experiment. The nitration reactions were carried out by orthogonal experiments and the optimum conditions were as follows: the ratio of resin to nitric acid is 12g/mL, the temperature of dropping nitric acid is -20°C and the time of stirring is 3h, the reaction temperature and time is 40°C and 1h, respectively; the conversion of o-xylene is 89%; the mass ratio of 4-nitro-o-xylene to 3-nitro-o-xylene is 1.81. The reusability of resin was good.


Ke Z.,Biopharmaceutical R and nter | Xu F.,Biopharmaceutical R and nter | Jiang J.,Biopharmaceutical R and nter | Xu D.,Zhejiang Jianfeng Haizhou Pharmaceutical Co.
Lizi Jiaohuan Yu Xifu/Ion Exchange and Adsorption | Year: 2013

The influencing factors of nitration of 2,6-di-tert-butylphenol (DTBP) were investigated by single factor experiment using strong acid ion-exchange resins (ResSO3H) and 35% nitric acid as nitrating agents. The nitration conditions were optimized by orthogonal experiments and the optimum conditions were obtained as follows: the ratio of resin to nitric acid was 12g/60mmol, the concentration of nitric acid was 35%, the amount of nitric acid was 1.20 times of the mole number of DTBP. The nitric acid was added by dropping into the mixture of DTBP and ResSO3H for about one hour at 0-5°C, then the system was heated at 10°C and reacted for another one hour until DTBP was completely reacted. The yield of the product 2,6-di-tert-butyl-4-nitrophenol (DTBNP) was above 90%. Besides, the resins had good reusability.

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