BIONT Inc.

Bratislava, Slovakia

BIONT Inc.

Bratislava, Slovakia

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Partelova D.,University of Trnava | Uhrovcik J.,University of Trnava | Lesny J.,University of Trnava | Hornik M.,University of Trnava | And 3 more authors.
Chemical Papers | Year: 2014

The aim of the presented work was to evaluate the uptake and distribution of 2-fluoro-2-deoxy-D-glucose spiked with 18F (2-[18F]FDG) in tissues of tobacco plants (Nicotiana tabacum L.) by positron emission tomography and multivariate data analysis after the immersion of the petiole of excised leaf or root of a tobacco plant in a glucose solution. From individual experiments it was found that increasing glucose concentration (cglu) in the applied solution resulted in significantly higher 2-[18F]FDG diffusion and translocation within the leaf parenchyma. More than a four times increase of the 2-[18F]FDG translocation into the aboveground parts of the tobacco plant in case of the root immersion in solution with 100-times higher cglu in comparison with the control (cglu = 0.00762 mg cm-3) was determined. These facts were not confirmed only visually on basis of the obtained 3D images, but also by the increasing coincidence transfer factor (TFC) values defined by the ratio of the number of analyzed coincidences in the non-immersed parts of leaf or plant to coincidences in leaf petiole or root immersed in the solution. Cluster and principal component analysis suggest that the 2-[18F]FDG uptake by the petiole of excised leaf and root system was realized by different mechanisms; also, the 3D image quality is influenced by the initial radioactivity of the applied solution. © 2014 Institute of Chemistry, Slovak Academy of Sciences.


Partelova D.,University of Trnava | Hornik M.,University of Trnava | Lesny J.,University of Trnava | Rajec P.,BIONT Inc. | And 2 more authors.
Applied Radiation and Isotopes | Year: 2016

In this work, a novel approach utilizing the designed phantoms imitating the plant tissues was applied for the evaluation of the relationships between the parameters of the prepared phantoms and/or quantitative variables obtained within the PET analysis. The microPET system developed for animal objects and approaches used made it possible to obtain the quantitative data in the form of 18F radioactivity as well as the glucose (in µg) accumulated in leaf tissues within the dynamic in vivo study. © 2016 Elsevier Ltd


Karade Y.,TU Eindhoven | Madani-Grasset F.,TU Eindhoven | Berger R.,Max Planck Institute for Polymer Research | Csiba V.,Biont Inc. | And 3 more authors.
Microelectronic Engineering | Year: 2010

A technique for fabricating pre-pattern induced phase-separated polymer blend films is presented. The gold layer coated silicon substrates were bombarded with focused ion beams (FIB) to sputter away Au grains in the irradiated regions and expose silicon oxide surface underneath, thereby creating pre-patterns of relatively hydrophobic (Au)/hydrophilic (silicon oxide) regions. In the pre-patterned regions, the spin-coated films consisting of immiscible PS (polystyrene) and PtBA (poly-tert-butyl acrylate) blend exhibited phase separation induced by the underlying pre-patterns and formed two distinct ordered morphologies with periodicities much smaller than that of the pre-patterns. The influence of varying periodicities of the pre-patterned structures, blending ratios and spin-coating parameters on the resulting morphologies are presented. © 2009 Elsevier B.V. All rights reserved.


Gasparova Z.,Slovak Academy of Sciences | Snirc V.,Slovak Academy of Sciences | Stolc S.,Slovak Academy of Sciences | Stolc S.,BIONT Inc. | And 3 more authors.
Neuroendocrinology Letters | Year: 2010

OBJECTIVE: Damage to the developing brain may be caused by maternal environment, nutritional deficiencies, failure of protective mechanisms, etc. Further, the developing brain may be damaged by intrauterine ischemia or by ischemia in newborns complicated by perinatal asphyxia. There is an effort to find agents with neuroprotective effect on the developing brain. The aim was to study the effect of the new pyridoindole antioxidant SMe1EC2 on the resistance of offspring hippocampus exposed to ischemia in vitro after treatment of mothers. MATERIALS AND METHODS: The electrically evoked responses were determined by extracellular recording from offspring hippocampal slices. The effect of oral treatment of rats with SMe1EC2 over 18 consecutive days, from day 15 of gestation to day 10 post partum (PP) was analyzed in the model of ischemia in vitro measured on the hippocampus of 21-day-old pups, with focus on neuronal function recovery in reoxygenation. RESULTS: Increased recovery of neuronal response was found at the end of 20-min reoxygenation in offspring hippocampal slices exposed to 10-min hypoxia/hypoglycemia from rats whose mothers were treated with the dose of 50 and 250 mg/kg of SMe1EC2, compared to control offspring slices (mothers received vehicle over the same time). CONCLUSIONS: The increased offspring hippocampus resistance to hypoxia/hypoglycemia due to 18-day maternal treatment with SMe1EC2 might have been obtained via the transplacental way as well as in the neonatal period via breast milk, skin and saliva. The manifested neuroprotective effect of SMe1EC2 on the developing brain might find exploitation during risk pregnancy and delivery. © 2010 Neuroendocrinology Letters.


Gasparova Z.,Slovak Academy of Sciences | Ondrejickova O.,Slovak Academy of Sciences | Gajdosikova A.,Slovak Academy of Sciences | Gajdosik A.,Slovak Academy of Sciences | And 3 more authors.
Interdisciplinary Toxicology | Year: 2010

New effective strategies and new highly effective neuroprotective agents are being searched for the therapy of human stroke and cerebral ischemia. The compound SMe1EC2 is a new derivative of stobadine, with enhanced antioxidant properties compared to the maternal drug. Carvedilol, a non-selective beta-blocker, possesses besides its cardioprotective and vasculoprotective properties also an antioxidant effect. We compared the effect of carvedilol and SMe1EC2, antioxidants with a similar chemical structure, in two experimental models of oxidative stress in young and adult rat brain tissue. SMe1EC2 was found to improve the resistance of hippocampal neurons to ischemia in vitro in young and even in 18-month-old rats and inhibited formation of protein carbonyl groups induced by the Fe2+/ascorbic acid pro-oxidative system in brain cortex homogenates of young rats. Carvedilol exerted a protective effect only in the hippocampus of 2-month-old rats and that at the concentration 10-times higher than did SMe1EC2. The inhibitory effect of carvedilol on protein carbonyl formation induced by the pro-oxidative system was not proved in the cortex of either young or adult rats. An increased baseline level of the content of protein carbonyl groups in the adult versus young rat brain cortex confirmed age-related changes in neuronal tissue and may be due to increased production of reactive oxygen species and low antioxidant defense mechanisms in the adult rat brain. The results revealed the new pyridoindole SMe1EC2 to be more effective than carvedilol in neuroprotection of rat brain tissue in both experimental models involving oxidative stress. Copyright © 2010 Slovak Toxicology Society SETOX.


Gasparova Z.,Slovak Academy of Sciences | Snirc V.,Slovak Academy of Sciences | Stolc S.,Slovak Academy of Sciences | Stolc S.,BIONT Inc.
Interdisciplinary Toxicology | Year: 2011

Previously, the pyridoindole SMe1EC2 was proved to inhibit lipoperoxidation and carbonylation of proteins in rat brain cortex in the system Fe 2+/ascorbate and improvement of resistance of the rat hippocampus was reported against ischemic conditions in vitro (hypoxia/hypoglycemia) expressed by the enhanced neuronal response recovery in reoxygenation. The hippocampus fulfils many of the criteria for a neuronal correlate of learning and memory. Recently, an impairment of hippocampal long-term potentiation (LTP) was reported under oxidative stress. Different therapies, including antioxidants, have been studied intensively concerning the impairment of neuronal plasticity. In this study marked reduction of LTP, elicited by a single burst (100 Hz, 1s) in the CA3-CA1 area of rat hippocampal slices, was shown due to transient hypoxia/hypoglycemia compared to control slices. On the basis of previously reported antioxidant and neuroprotective effects of SMe1EC2, its effect on loss of LTP in the hippocampus due to ischemic conditions was studied in vitro. The pyridoindole tested improved hypoxia/hypoglycemia-induced reduction of LTP compared to untreated hypoxic slices. An opposite effect of SMe1EC2 on LTP induction was found in control slices. The mechanism of SMe1EC2 action on LTP in ischemic conditions has been suggested to differ from the mechanism of its effect in "normoxia" and may be due to different redox status in control and ischemic brain tissue. The manifested LTP-protective effect of SMe1EC2 observed in the rat hippocampus exposed to ischemia in vitro may find exploitation in therapy associated with injured neuronal plasticity in some conditions, including ischemia, trauma and aging in man. Copyright © 2011 Slovak Toxicology Society SETOX.


Stolc S.,Biont Inc. | Jakubikova L.,Biont Inc. | Kukurova I.,Biont Inc.
Interdisciplinary Toxicology | Year: 2011

Compounds 18F-fluorodeoxyglucose (18FDG) and 11C-methionine (11C-MET) are radiodiagnostics frequently used in clinical Positron Emission Tomography (PET) as well in preclinical studies of various pathologies. The present study was focused on the comparison of biodistribution of both radiotracers in intact Wistar rats. The animals were scanned by microPET twice. The first scanning was done after 11C-MET administration, the second scan followed 5-7 days later using 18FDG. The radiotracers were injected into the tail vein of animals in isoflurane anesthesia. After a redistribution period, whole body scans were obtained using eXplore Vista SrT GE tomograph. Accumulation of the drugs in tissues was expressed in relative values (% ID/g) in selected regions of interest. As arbitrary reference tissue for drug accumulation, the sternoclavicular area was used. 18C-MET was found remarkably cumulating especially in the liver, spleen and distal part of the gastrointestinal tract. The compound was accumulated in the liver 6.9 ± 0.92 (mean ± SEM) times more intensively than in the reference tissue. The respective value for spleen and cecum/colon was 5.62 ± 0.81 and 3.56 ± 0.14 times. Accumulation of 11C-MET in other body parts including the brain and heart was very low and was apparently equal to the arbitrary tissue (0.13 ± 0.01% ID/g). In the same animals 18FDG (biontFDG) was remarkably cumulated especially in Harderian glands compared to arbitrary tissue background (11.02 ± 1.00 times), heart (7.52 ± 1.70 times), brain (6.14 ± 0.37 times), and colon (5.68 ± 0.31 times). 18FDG accumulation in the liver, spleen and other organs was apparently not different from that found in the background (0.14 ± 0.02% ID/g). The data obtained may serve as reference values in further microPET preclinical studies with 11C-MET and 18FDG under the given conditions. Copyright © 2011 Slovak Toxicology Society SETOX.


PubMed | Biont Inc.
Type: Journal Article | Journal: Interdisciplinary toxicology | Year: 2011

Compounds (18)F-fluorodeoxyglucose ((18)FDG) and (11)C-methionine ((11)C-MET) are radiodiagnostics frequently used in clinical Positron Emission Tomography (PET) as well in preclinical studies of various pathologies. The present study was focused on the comparison of biodistribution of both radiotracers in intact Wistar rats. The animals were scanned by microPET twice. The first scanning was done after (11)C-MET administration, the second scan followed 5-7 days later using (18)FDG. The radiotracers were injected into the tail vein of animals in isoflurane anesthesia. After a redistribution period, whole body scans were obtained using eXplore Vista SrT GE tomograph. Accumulation of the drugs in tissues was expressed in relative values (% ID/g) in selected regions of interest. As arbitrary reference tissue for drug accumulation, the sternoclavicular area was used. (18)C-MET was found remarkably cumulating especially in the liver, spleen and distal part of the gastrointestinal tract. The compound was accumulated in the liver 6.90.92 (meanSEM) times more intensively than in the reference tissue. The respective value for spleen and cecum/colon was 5.620.81 and 3.560.14 times. Accumulation of (11)C-MET in other body parts including the brain and heart was very low and was apparently equal to the arbitrary tissue (0.130.01% ID/g). In the same animals (18)FDG (biontFDG) was remarkably cumulated especially in Harderian glands compared to arbitrary tissue background (11.021.00 times), heart (7.521.70 times), brain (6.140.37 times), and colon (5.680.31 times). (18)FDG accumulation in the liver, spleen and other organs was apparently not different from that found in the background (0.140.02% ID/g). The data obtained may serve as reference values in further microPET preclinical studies with (11)C-MET and (18)FDG under the given conditions.

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