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Neumarkt in der Oberpfalz, Germany

Cheilari A.,University of Innsbruck | Sturm S.,University of Innsbruck | Intelmann D.,Bionorica GmbH | Seger C.,University of Innsbruck | Stuppner H.,University of Innsbruck
Journal of Agricultural and Food Chemistry

Quantitative nuclear magnetic resonance (qNMR) spectroscopy is known as an excellent alternative to chromatography-based mixture analysis. NMR spectroscopy is a non-destructive method, needs only limited sample preparation, and can be readily automated. A head-to-head comparison of qNMR to an ultra-high-performance liquid chromatography with diode array detection (uHPLC-DAD)-based quantitative analysis of six flavonolignan congeners (silychristin, silydianin, silybin A, silybin B, isosilybin A, and isosilybin B) of the Silybum marianum silymarin complex is presented. Both assays showed similar performance characteristics (linear range, accuracy, precision, and limits of quantitation) with analysis times below 30 min/sample. The assays were applied to industrial S. marianum extracts (AC samples) and to extracts locally prepared from S. marianum fruits (PL samples). An assay comparison by Bland-Altman plots (relative method bias AC samples, -0.1%; 2SD range, ±5.1%; relative method bias PL samples, -0.3%; 2SD range, ±7.8%) and Passing-Bablok regression analysis (slope and intercept for AC and PL samples not significantly different from 1.00 and 0.00, respectively; Spearman's coefficient of rank correlation, >0.99) did show that qNMR and uHPLC-DAD can be used interchangeably to quantitate flavonolignans in the silymarin complex. © 2016 American Chemical Society. Source

Mattle C.,Institute for Radiochemistry | Heigl N.,Institute for Radiochemistry | Abel G.,Bionorica GmbH | Bonn G.K.,Institute for Radiochemistry | Huck C.W.,Institute for Radiochemistry
Journal of Planar Chromatography - Modern TLC

By use of near infrared (NIR) spectroscopy hyphenated to thinlayer chromatography (TLC) for analysis of methoxylated flavones in a phytomedicine we sought to achieve two objectives: first, to establish a method for rapid, qualitative identification of five methoxylated flavones, denoted G1, G2, G3, G4, and G5, in order of their R F values in normal-phase TLC, and, second, to produce a quantitative model for analysis of G4 (3′,4′, 5′-trimethoxyflavone), the compound most representative of Primula veris flowers in phytomedicine. To provide appropriate reference analytical data for building the multivariate cluster and partial least-squares regression (PLS) model, TLC was performed on alumina with n -hexane-ethyl acetate 70:30 (v/v) as mobile phase. Forty-four spectra of eleven independent phytomedicine samples were analyzed with five scans to generate a qualitative cluster model based on PCA (principle-components analysis) that enabled differentiation between G1-G5 on the basis of their methoxylation pattern. This PLS model, in the calibration range between 0 and 1000 mg L -1, enabled quantification of G4 with a standard error of cross validation (SECV),54.61 mg L -1 . The possibility of conducting qualitative and quantitative analysis simultaneously by use of this method revealed NIRS to be an efficient alternative to conventional modes of detection used for analysis of G1-G5, especially in phytomedicines. © Akadémiai Kiadó, Budapest. Source

Qureshi M.N.,Abdul Wali Khan University Mardan | Qureshi M.N.,University of Innsbruck | Stecher G.,University of Innsbruck | Stecher G.,Bionorica GmbH | Bonn G.K.,University of Innsbruck
Analytical Letters

The standardization of natural product drugs needs reliable methods for the analysis of raw materials and final products. In this study, qualitative analysis of amino acids in herbal extracts was performed using mass spectrometric method, that is, matrix-free material enhanced laser desorption ionization time of flight mass spectrometry (mf-MELDI-MS). Samples employed in the study were standards and water extracts from Althaea officinalis, Taraxacum officinale, and Matricaria chamomilla.These analyses confirmed the presence of amino acids and clearly proved the adequate performance of mf-MELDI-MS for the qualitative analysis of complex mixtures, as targets do not need modification and analysis requires only a few minutes. Determination of amino acids in these plants were performed and reported for the first time using the modified silica gel (4,4′-azodianiline modified silica gel) and mf-MELDI-MS technique. © 2013 Copyright Taylor and Francis Group, LLC. Source

Bionorica GmbH | Date: 2012-12-11

Printed matter, namely, books, journals, leaflets and brochures in the fields of health and medicine; instructional and teaching material, namely, instructional and teaching materials in the field of medicine and phytopharmacy.

The present invention relates to an antibacterial agent and a hydrolysate made of at least one extract that has been produced by extraction using ethanol/water from dried plant material of: a) at least one of the plants selected from the group consisting of:

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