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Weltin A.,Albert Ludwigs University of Freiburg | Weltin A.,Laboratory for Sensors | Slotwinksi K.,Albert Ludwigs University of Freiburg | Kieninger J.,Albert Ludwigs University of Freiburg | And 4 more authors.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 | Year: 2013

A novel multiparametric microsensor system for online cell culture monitoring with human tumor cells is introduced. The system combines cell culture, microiluidics and biosensors on a single chip. It features pH, oxygen, glucose and lactate sensors on a transparent chip. The sensor performance was proven in cell culture environment. Cellular metabolism of T98G brain tumor cells was measured. Extracellular acidification and respiration can be quantified. Medium was efficiently transported to the biosensors by microiluidics. Glucose consumption and lactate production was measured in cycles with low analyte volumes (< 15 μl). Alteration of cellular metabolism by compounds could be monitored successfully. Source

Eisenloffel C.,University of Rostock | Schmole A.-C.,University of Rostock | Pews-Davtyan A.,Leibniz Institute for Catalysis at the University of Rostock | Brennfuhrer A.,Leibniz Institute for Catalysis at the University of Rostock | And 9 more authors.
Biochemical Pharmacology | Year: 2013

Indolylmaleimides display a broad spectrum of biological activity and offer great opportunity to influence several aspects of cell fate, as proliferation and differentiation. In this study we describe the effect of PDA-66, a newly synthesised indolylmaleimide, showing a strong dose dependent anti-proliferative effect on immortalised human progenitor and cancer cells. We demonstrated a highly depolymerizing effect on in vitro tubulin assembly and conclude that PDA-66 acts as microtubule destabilising agent. In addition we found that PDA-66 induces mitotic arrest of cells in the G2/M phase of the cell cycle. Subsequently cells undergo apoptosis, indicating the major mechanism of the anti-proliferative effect. To prove a potential anti-cancer activity of PDA-66 we examined the effect of PDA-66 on human SH-SY5Y neuroblastoma and A-459 lung cancer cells, showing a significant reduction in cancer cell proliferation in a dose dependent manner. Thus PDA-66 is a new anti-mitotic compound with an indole-core with the potential to be used for cancer therapy. © 2012 Elsevier Inc. Source

Drechsler S.,Bionas GmbH | Andra J.,Research Center Borstel
Journal of Bioenergetics and Biomembranes | Year: 2011

Antimicrobial peptides are promising anti-cancer agents with a unique mode of action. We established the usage of a chip-based sensor to monitor the dynamic interplay between cells on the chip and peptides and compared it with endpoint tests. Human neuroblastoma cancer cells and spontaneously immortalized non-cancer keratinocytes were perfused with representative peptides (NK-2, NK11, and melittin). The sensor system enabled continuous recording of cell layer impedance (adhesion/confluence), oxygen consumption (respiration) and extracellular acidification (glycolysis) and provided insights in cell damage, stress response and recovery. Cells responded differentially to peptide treatment. During perfusion, peptides accumulated on the cell surface until they reached a critical concentration. Preceding to cell death, melittin triggered glycolysis, suggesting stress response. NK-2 induced no change in energy metabolism, but led to an increase in impedance, i.e. a temporarily altered morphology, which appeared to be an excellent parameter to detect subtle structural changes of cell layers. © 2011 The Author(s). Source

Thakur M.,Charite - Medical University of Berlin | Mergel K.,Charite - Medical University of Berlin | Weng A.,Charite - Medical University of Berlin | Frech S.,Bionas GmbH | And 4 more authors.
Biosensors and Bioelectronics | Year: 2012

This work describes the application of an impedance-based measurement for the real time evaluation of targeted tumor therapies in cell culture (HeLa cells). We used a treatment procedure that is well established in cells and mice. Therein, tumor cells are treated with a combination of an epidermal growth factor-based targeted toxin named SE and particular plant glycosides called saponins. In the present study HeLa cells were seeded in different numbers onto interdigitated electrode structures integrated into the bottom of a 96 well plate. The cells were treated with SE in the presence and absence of the saponin SpnS-1 (isolated from Saponaria officinalis roots). The impedance was directly correlated with the viability of the cells. As expected from known end point measurements, a concentration dependent enhancement of toxicity was observed; however, with the impedance measurement we were for the first time able to trace the temporal changes of cell death during the combination treatment. This substantially added to the understanding of initial cellular mechanisms in the augmentation of the toxicity of targeted toxins by saponins and indicated the superiority of real time monitoring over end point assays. The method is less labor intensive and label-free with ease of monitoring the effects at each time point. © 2012 Elsevier B.V. Source

Bionas GmbH | Date: 2005-03-03

measuring apparatus and measuring instruments, namely, measuring apparatus and measuring instruments including semiconductor chips in combination with live neuronal tissue for determining effects of various substances on neurons and control and data analysis apparatus for use with the chips. scientific testing of neuronal tissue.

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