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Flora R.,University of Sriwijaya | Zulkarnain M.,University of Sriwijaya | Sorena E.,Medical Polytechnic of Bengkulu | Deva I.D.G.S.,Biomolecular and Biomedical Research Center | Widowati W.,Maranatha Christian University
Trends in Medical Research | Year: 2016

During physical activity, oxygen reduction leading to hypoxia affects brain cell metabolisms, induces stabilization of Hypoxia Inducible Factor-1α (HIF-1α) and up-regulate Vesicular Endothelial Growth Factor (VEGF). This study aimed to investigate the correlation of HIF-1α and VEGF of brain tissue in male wistar rat after anaerobic exercise. Twenty four rats were divided into four groups: control, 1x, 3x and 7x in a week of anaerobic exercise. A rat treadmill was used at speed 35 m min-1, 20 min for every anaerobic exercise. The HIF-1α and VEGF level were measured by ELISA. The correlation between HIF-1α and VEGF was analyzed using Pearson test. The HIF-1α and VEGF level increased in 1x and 3x a week of anaerobic exercise. The highest HIF-1α and VEGF level were observed in 1x a week of anaerobic exercise. Pearson test between HIF-1α and VEGF showed r = 0.709 and p = 0.000. These findings showed that HIF-1α and VEGF are correlated and anaerobic exercise affects HIF-1α and VEGF level. © 2016 Academic Journals Inc.

Widowati W.,Maranatha Christian University | Wijaya L.,Stem Cell and Cancer Institute | Agustina D.,Stem Cell and Cancer Institute | Murti H.,Stem Cell and Cancer Institute | And 4 more authors.
International Journal of Cancer Research | Year: 2015

Cancer is one of the leading causes of mortality and morbidity throughout the world. Since there are still some problems related to the conventional therapies for cancer treatment, it is critical to explore new more efficient therapy strategies. Mesenchymal Stem Cells (MSCs) are one of powerful tools for tissue engineering for regenerative medicine, as recent research aims to utilize MSCs for anti-cancer treatment. Our previous research demonstrated that Conditioned Medium from Whartons’ Jelly MSCs (WJ-MSCs-CM) significantly lowered cancer proliferation of various cancer cell lines. This research was performed to evaluate the tumoricidal property of cell lysate from WJ-MSCs from normoxia (WJMSCs-norCL) and hypoxia-treated WJMSCs (WJMSCs-hypoCL) on the proliferation of human cancer cells, including cervical (HeLa), liver (HepG2), ovarian (SKOV3) and oral squamous (HSC3) cancer cell lines compared to normal cells including mouse fibroblast (NIH3T3), human Mesenchymal Stem Cells (hMSCs), human fibroblast. The WJMSCs-norCL and WJMSCs-hypoCL have cytotoxic activity, reduce proliferation of various cancer cell lines with minimum inhibitory concentration (IC50) 21.094-95.928 μg mLG1 and no cytotoxic to normal cells with IC50 409, 191-629, 799.738 μg mL- 1. The WJMSCs-norCL and WJMSCs-hypoCL inhibit proliferation in various cancer cell lines and are not toxic for normal cells. © 2015 Academic Journals Inc.

Widowati W.,Maranatha Christian University | Wijaya L.,Stem Cell and Cancer Institute | Bachtiar I.,Stem Cell and Cancer Institute | Gunanegara R.F.,Maranatha Christian University | And 4 more authors.
Biomarkers and Genomic Medicine | Year: 2014

Mesenchymal stem cells (MSCs) from Wharton's jelly have a higher proliferation rate and self-renewal capacity than adult tissue-derived MSCs. A low oxygen level or hypoxic condition is prevalent in the microenvironment of the stem cells in the early stages of development. Hypoxia can influence proliferation and differentiation of various stem/precursor cell populations. This research was conducted: to determine the proliferation rate and characteristics of human MSCs from Wharton's jelly in hypoxic and normoxic condition; to evaluate their character after MSCs are incubated in hypoxic and normoxic environment using surface markers including CD105, CD73, CD14, CD19, CD34, CD45, and HLA-II; and to evaluate the proliferation rate and number of MSCs at many passages using the trypan blue method. The hypoxic and normoxic microenvironment showed significant differences in the proliferation rate and population doubling time, but and there were no differences in surface markers. © 2014.

Widowati W.,Maranatha Christian University | Widyanto R.M.,Biomolecular and Biomedical Research Center | Husin W.,Maranatha Christian University | Ratnawati H.,Maranatha Christian University | And 4 more authors.
Iranian Journal of Basic Medical Sciences | Year: 2014

Objective(s): Many studies have reported that tea consumption decreases cardiovascular risk, but the mechanisms remain unclear. Green tea is known to have potent antioxidant and free radical scavenging activities. This study aimed to investigate whether green tea extract (GTE) can protect endothelial progenitors cells (EPCs) against oxidative stress through antioxidant mechanisms.Materials and Methods: Mononuclear cells (MNCs) were isolated from peripheral blood by density gradient centrifugation with Ficoll. The cells were then plated on fibronectin-coated culture dishes. After 7 days of culture, EPCs were characterized as adherent cells double positive for DiI-ac-LDL uptake and lectin binding. EPCs were further identified by assessing the expression of CD34/45, CD133, and KDR. EPCs were then treated with hydrogen peroxide (H2O2) at doses of 50, 100, 200 μM and incubated with or without GTE (25 μg/ml). The intracellular reactive oxygen species (ROS) levels were detected by flow cytometry using a 2’,7’-dichlorofluorescein diacetate (DCF-DA) fluorescent probe.Results: GTE ameliorated the cell viability of EPCs induced by H2O2 at doses of 50, 100, 200 μM for about 25.47, 22.52, and 11.96% higher than controls, respectively. GTE also decreased the intracellular ROS levels of EPCs induced by H2O2 at doses of 50, 100, 200 μM for about 84.24, 92.27, and 93.72% compared to controls, respectively.Conclusion: GTE improves cell viability by reducing the intracellular ROS accumulation in H2O2-induced EPCs. © 2014 - Journal Management System.

Nurhayati B.,Bandung Institute of Technology | Wibowo M.S.,Bandung Institute of Technology | Widyastuti Y.,Indonesian Institute of Sciences | Erawijantari P.P.,Biomolecular and Biomedical Research Center | And 4 more authors.
Research Journal of Microbiology | Year: 2015

Lactobacillus plantarum species often harbor several plasmids. These plasmids may encoded important traits such as phages or antibiotics resistances, lactose catabolism and production of proteolytic enzyme and also bacteriocins that named plantaricin. Lactobacillus plantarum IBL-2 that isolated from strawberry of Bali plantation have the highest anti-microorganism activity among the L. plantarum isolate collection of BTCC Indonesia. Only few study carried out to examine the antifungal activity of bacteriocin from L. plantarum. This study focus on anti-Candida activity of plasmid associated with bacteriocin production from L. plantarum IBL-2. The isolates were confirmed by the 16S rRNA analysis by PCR and the phylogenetic tree was built based on references sequences and one outgroups from database. The plantaricin gene screening then performed in plasmid that was isolated from L. plantarum IBL-2 by PCR using five pairs or plantaricin gene primer. The anti-Candida potential of plantaricin observed were analyzed by in silico by docking analysis between the plantaricin and receptors of apoptosis proteins. PlnB and PlnEF were observed in the L. plantarum plasmid. Only PlnEF become the focus of study. Analysis of docking study predicted that PlnEF have interactions with apoptosis proteins regulator in eukaryotic cells. PlnEF that encoded by plasmid of L. plantarum may exert anti-Candida potential through interactions with apoptosis proteins regulator. © 2015 Academic Journals Inc.

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