Biomolecular and Biomedical Research Center

Bandung, Indonesia

Biomolecular and Biomedical Research Center

Bandung, Indonesia

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PubMed | Maranatha Christian University, Padjadjaran University, Jeju National University, Chungnam National University and Biomolecular and Biomedical Research Center
Type: Journal Article | Journal: Planta medica | Year: 2016

Four new compounds, acacetin 8-C-[


Thao N.P.,Vietnam Academy of Science and Technology | Luyen B.T.T.,Chungnam National University | Widowati W.,Maranatha Christian University | Fauziah N.,Biomolecular and Biomedical Research Center | And 6 more authors.
Planta Medica | Year: 2016

Four new compounds, acacetin 8-C-[β-D-apiofuranosyl-(1 → 2)-β-D-glucopyranoside] (1), 7-methoxyacacetin 8-C-[β-D-apiofuranosyl-(1 → 3)-β-D-glucopyranoside] (2), 7-methoxyacacetin 8-C-[β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranoside] (3), and 4‴-O-acetylacacetin 8-C-[α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside] (4), along with ten known compounds (5–14), were isolated from Piper aduncum leaves. The effects of these compounds on lipopolysaccharide-induced expression of the proinflammatory cytokines IL-12 p40, IL-6, and TNF-α in bone marrow-derived dendritic cells were evaluated. Compounds 2, 3, 6, 8, 9, and 11–13 inhibited the production of both IL-12 p40 and IL-6, with IC50 values ranging from 0.35 ± 0.01 to 1.40 ± 0.04 µM and 1.22 ± 0.02 to 3.79 ± 0.10 µM, respectively. Compounds 5 and 10 only showed strong inhibition effects on the production of IL-12 p40, with IC50 values of 2.76 ± 0.08 and 0.39 ± 0.05 µM, respectively. However, all compounds showed weak activity or no activity on TNF-α production at the tested concentrations. Copyright © 2016, Georg Thieme Verlag KG. All rights reserved.


Widowati W.,Maranatha Christian University | Wijaya L.,Stem Cell and Cancer Institute | Laksmitawati D.R.,Pancasila University | Widyanto R.M.,Brawijaya University | And 5 more authors.
Natural Product Sciences | Year: 2016

Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 µmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels. © 2016 Korean Society of Pharmacognosy. All rights reserved.


Widowati W.,Maranatha Christian University | Widyanto R.M.,Biomolecular and Biomedical Research Center | Husin W.,Maranatha Christian University | Ratnawati H.,Maranatha Christian University | And 4 more authors.
Iranian Journal of Basic Medical Sciences | Year: 2014

Objective(s): Many studies have reported that tea consumption decreases cardiovascular risk, but the mechanisms remain unclear. Green tea is known to have potent antioxidant and free radical scavenging activities. This study aimed to investigate whether green tea extract (GTE) can protect endothelial progenitors cells (EPCs) against oxidative stress through antioxidant mechanisms.Materials and Methods: Mononuclear cells (MNCs) were isolated from peripheral blood by density gradient centrifugation with Ficoll. The cells were then plated on fibronectin-coated culture dishes. After 7 days of culture, EPCs were characterized as adherent cells double positive for DiI-ac-LDL uptake and lectin binding. EPCs were further identified by assessing the expression of CD34/45, CD133, and KDR. EPCs were then treated with hydrogen peroxide (H2O2) at doses of 50, 100, 200 μM and incubated with or without GTE (25 μg/ml). The intracellular reactive oxygen species (ROS) levels were detected by flow cytometry using a 2’,7’-dichlorofluorescein diacetate (DCF-DA) fluorescent probe.Results: GTE ameliorated the cell viability of EPCs induced by H2O2 at doses of 50, 100, 200 μM for about 25.47, 22.52, and 11.96% higher than controls, respectively. GTE also decreased the intracellular ROS levels of EPCs induced by H2O2 at doses of 50, 100, 200 μM for about 84.24, 92.27, and 93.72% compared to controls, respectively.Conclusion: GTE improves cell viability by reducing the intracellular ROS accumulation in H2O2-induced EPCs. © 2014 - Journal Management System.


PubMed | Maranatha Christian University, Lambung Mangkurat University, Biomolecular and Biomedical Research Center, Pancasila University and 2 more.
Type: Journal Article | Journal: Iranian journal of basic medical sciences | Year: 2015

Many studies have reported that tea consumption decreases cardiovascular risk, but the mechanisms remain unclear. Green tea is known to have potent antioxidant and free radical scavenging activities. This study aimed to investigate whether green tea extract (GTE) can protect endothelial progenitors cells (EPCs) against oxidative stress through antioxidant mechanisms.Mononuclear cells (MNCs) were isolated from peripheral blood by density gradient centrifugation with Ficoll. The cells were then plated on fibronectin-coated culture dishes. After 7 days of culture, EPCs were characterized as adherent cells double positive for DiI-ac-LDL uptake and lectin binding. EPCs were further identified by assessing the expression of CD34/45, CD133, and KDR. EPCs were then treated with hydrogen peroxide (H2O2) at doses of 50, 100, 200 M and incubated with or without GTE (25 g/ml). The intracellular reactive oxygen species (ROS) levels were detected by flow cytometry using a 2,7-dichlorofluorescein diacetate (DCF-DA) fluorescent probe.GTE ameliorated the cell viability of EPCs induced by H2O2 at doses of 50, 100, 200 M for about 25.47, 22.52, and 11.96% higher than controls, respectively. GTE also decreased the intracellular ROS levels of EPCs induced by H2O2 at doses of 50, 100, 200 M for about 84.24, 92.27, and 93.72% compared to controls, respectively.GTE improves cell viability by reducing the intracellular ROS accumulation in H2O2-induced EPCs.


Flora R.,University of Sriwijaya | Zulkarnain M.,University of Sriwijaya | Sorena E.,Medical Polytechnic of Bengkulu | Deva I.D.G.S.,Biomolecular and Biomedical Research Center | Widowati W.,Maranatha Christian University
Trends in Medical Research | Year: 2016

During physical activity, oxygen reduction leading to hypoxia affects brain cell metabolisms, induces stabilization of Hypoxia Inducible Factor-1α (HIF-1α) and up-regulate Vesicular Endothelial Growth Factor (VEGF). This study aimed to investigate the correlation of HIF-1α and VEGF of brain tissue in male wistar rat after anaerobic exercise. Twenty four rats were divided into four groups: control, 1x, 3x and 7x in a week of anaerobic exercise. A rat treadmill was used at speed 35 m min-1, 20 min for every anaerobic exercise. The HIF-1α and VEGF level were measured by ELISA. The correlation between HIF-1α and VEGF was analyzed using Pearson test. The HIF-1α and VEGF level increased in 1x and 3x a week of anaerobic exercise. The highest HIF-1α and VEGF level were observed in 1x a week of anaerobic exercise. Pearson test between HIF-1α and VEGF showed r = 0.709 and p = 0.000. These findings showed that HIF-1α and VEGF are correlated and anaerobic exercise affects HIF-1α and VEGF level. © 2016 Academic Journals Inc.


Widowati W.,Maranatha Christian University | Murti H.,Stem Cell and Cancer Institute | Jasaputra D.K.,Maranatha Christian University | Sumitro S.B.,Brawijaya University | And 4 more authors.
Asian Journal of Cell Biology | Year: 2016

Background: Breast cancer is one of the most common life threatening diseases worldwide and it is the leading cause of death from cancer in women. The effectiveness of current cancer therapies is low, even though it requires expensive cost. Therefore, the development of the more efficient therapy is highly needed. Objective: This research was conducted to evaluate the effect Tumour Necrosis Factor alpha (TNF-α) and Interferon gamma (IFN-γ) as anticancer agents against breast cancer cells (T47D, MCF7) and selectivity of cytotoxic effect towards human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) to produce hWJMSCs-Conditioned Medium (CM), hWJMSCs-Cell Lysate (CL) and hWJMSCs which potential used as anticancer candidates after induced by TNFα or IFNγ. Methodology: The hWJMSCs were isolated from umbilical cord and characterized by its surface marker phenotype and its multipotent differentiation potential. The breast cancer cell lines (T47D and MCF7) were treated by TNF-α and IFN-γ and its cytotoxic activity was observed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS) viability assay. The cytotoxic effects of TNF-α and IFN-γ toward hWJMSCs were also observed using the same method. Results: Flow cytometry analysis showed that hWJMSCs for early passage (P4) were positive for hMSCs marker CD105, CD73 and negative for CD34, CD45, CD14, CD19 and HLA-II. It also showed the osteogenic, adipogenic and chondrogenic differentiation. The effect of TNF-α and IFN-γ against MCF7 cells exhibited that the cytokines decreased the cell viability in a dose dependent manner. The IC50 value of TNF-α and IFN-γ against breast cancer cell lines were found higher for T47D than MCF7. Conclusion: The TNF-α and IFN-γ inhibit the cell growth of breast cancer cells due to apoptotic or necrotic cells but it non-toxic against hWJMSCs. © 2016 Wahyu Widowati et al.


Widowati W.,Maranatha Christian University | Wijaya L.,Stem Cell and Cancer Institute | Bachtiar I.,Stem Cell and Cancer Institute | Gunanegara R.F.,Maranatha Christian University | And 4 more authors.
Biomarkers and Genomic Medicine | Year: 2014

Mesenchymal stem cells (MSCs) from Wharton's jelly have a higher proliferation rate and self-renewal capacity than adult tissue-derived MSCs. A low oxygen level or hypoxic condition is prevalent in the microenvironment of the stem cells in the early stages of development. Hypoxia can influence proliferation and differentiation of various stem/precursor cell populations. This research was conducted: to determine the proliferation rate and characteristics of human MSCs from Wharton's jelly in hypoxic and normoxic condition; to evaluate their character after MSCs are incubated in hypoxic and normoxic environment using surface markers including CD105, CD73, CD14, CD19, CD34, CD45, and HLA-II; and to evaluate the proliferation rate and number of MSCs at many passages using the trypan blue method. The hypoxic and normoxic microenvironment showed significant differences in the proliferation rate and population doubling time, but and there were no differences in surface markers. © 2014.


Widowati W.,Maranatha Christian University | Wijaya L.,Stem Cell and Cancer Institute | Agustina D.,Stem Cell and Cancer Institute | Murti H.,Stem Cell and Cancer Institute | And 4 more authors.
International Journal of Cancer Research | Year: 2015

Cancer is one of the leading causes of mortality and morbidity throughout the world. Since there are still some problems related to the conventional therapies for cancer treatment, it is critical to explore new more efficient therapy strategies. Mesenchymal Stem Cells (MSCs) are one of powerful tools for tissue engineering for regenerative medicine, as recent research aims to utilize MSCs for anti-cancer treatment. Our previous research demonstrated that Conditioned Medium from Whartons’ Jelly MSCs (WJ-MSCs-CM) significantly lowered cancer proliferation of various cancer cell lines. This research was performed to evaluate the tumoricidal property of cell lysate from WJ-MSCs from normoxia (WJMSCs-norCL) and hypoxia-treated WJMSCs (WJMSCs-hypoCL) on the proliferation of human cancer cells, including cervical (HeLa), liver (HepG2), ovarian (SKOV3) and oral squamous (HSC3) cancer cell lines compared to normal cells including mouse fibroblast (NIH3T3), human Mesenchymal Stem Cells (hMSCs), human fibroblast. The WJMSCs-norCL and WJMSCs-hypoCL have cytotoxic activity, reduce proliferation of various cancer cell lines with minimum inhibitory concentration (IC50) 21.094-95.928 μg mLG1 and no cytotoxic to normal cells with IC50 409, 191-629, 799.738 μg mL- 1. The WJMSCs-norCL and WJMSCs-hypoCL inhibit proliferation in various cancer cell lines and are not toxic for normal cells. © 2015 Academic Journals Inc.


Rusmana D.,Maranatha Christian University | Elisabeth M.,Maranatha Christian University | Widowati W.,Maranatha Christian University | Fauziah N.,Biomolecular and Biomedical Research Center | Maesaroh M.,Biomolecular and Biomedical Research Center
Research Journal of Medicinal Plant | Year: 2015

Various plants and their derived compounds have been used in the treatment of inflammation, including Syzygium aromaticum (L.) and eugenol as one of the most active compounds in it. Inflammation is one of an important biological response to injury. Anti-inflammatory is important to treat the danger of acute and chronic inflammation. The objective of the present study was to evaluate anti-inflammatory potential of S. aromaticum flower bud (clove) ethanol extract and eugenol from S. aromaticum on LPS stimulated-murine macrophage cell line (RAW 264.7). Cell viability assay was performed to evaluate the nontoxic concentration in cell line was performed by MTS assay. The cytotoxic activity which is considered safe on RAW.264.7 cell were 50 and 10 μg mLG1 concentration of the S. aromaticum flower bud ethanol extract and eugenol. Several measurements were performed including IL-1β, TNF-α, NO and IL-6 concentration assay by Elisa after treatment compared to non treated cells to determine the anti-inflammatory activity. Syzygium aromaticum was inhibited IL-1β, TNF-α, NO and IL-6 release on LPS stimulated-RAW.264.7. No significant difference was observed in the S. aromaticum flower bud extract and eugenol on IL-1β inhibitory activity but eugenol showed the higher TNF-α inhibitory activity then S. aromaticum flower bud extract. The 50 μg mLG1 ethanol extract of S. aromaticum flower bud showed the highest IL-6 and nitrite-associated NO inhibitory activity. This research revealed that ethanol extract and eugenol of S. aromaticum flower bud (clove) possess the anti-inflammatory potential showed by the inhibitory activity of the inflammatory mediator including IL-1β, TNF-α, NO and IL-6. © 2015 Academic Journals Inc.

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