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Puchberger-Enengl D.,Vienna University of Technology | Krutzler C.,Austrian Center for Medical Innovation and Technology | Binder M.,Medical University of Vienna | Rohrer C.,Lohmann and Rauscher GmbH | And 3 more authors.
Procedia Engineering | Year: 2012

Conventional principles of clinical wound care rely on subjective assessments only, whereas objective measurements are hardly applied. In this contribution we present a novel sensor system for the integration into a wound dressing that measures healing related parameters in a continuous manner. The low-cost sensor determines wound pH, moisture, temperature, optical tissue absorption and oxygen saturation. The performance of the sensor elements is evaluated by different on-body experiments and reference measurements. © 2012 The Authors. Published by Elsevier Ltd.

Stadlbauer V.,Upper Austria University of Applied Sciences | Haselgrubler R.,Upper Austria University of Applied Sciences | Lanzerstorfer P.,Upper Austria University of Applied Sciences | Plochberger B.,Upper Austria University of Applied Sciences | And 7 more authors.
PLoS ONE | Year: 2016

Induction of GLUT4 translocation in the absence of insulin is considered a key concept to decrease elevated blood glucose levels in diabetics. Due to the lack of pharmaceuticals that specifically increase the uptake of glucose from the blood circuit, application of natural compounds might be an alternative strategy. However, the effects and mechanisms of action remain unknown for many of those substances. For this study we investigated extracts prepared from seven different plants, which have been reported to exhibit anti-diabetic effects, for their GLUT4 translocation inducing properties. Quantitation of GLUT4 translocation was determined by total internal reflection fluorescence (TIRF) microscopy in insulin sensitive CHO-K1 cells and adipocytes. Two extracts prepared from purslane (Portulaca oleracea) and tindora (Coccinia grandis) were found to induce GLUT4 translocation, accompanied by an increase of intracellular glucose concentrations. Our results indicate that the PI3K pathway is mainly responsible for the respective translocation process. Atomic force microscopy was used to prove complete plasma membrane insertion. Furthermore, this approach suggested a compound mediated distribution of GLUT4 molecules in the plasma membrane similar to insulin stimulated conditions. Utilizing a fluorescent actin marker, TIRF measurements indicated an impact of purslane and tindora on actin remodeling as observed in insulin treated cells. Finally, in-ovo experiments suggested a significant reduction of blood glucose levels under tindora and purslane treated conditions in a living organism. In conclusion, this study confirms the anti-diabetic properties of tindora and purslane, which stimulate GLUT4 translocation in an insulin-like manner. © 2016 Stadlbauer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Schafer C.,Biomedical zet Life Science GmbH | Schroder K.R.,Biomedical zet Life Science GmbH | Hoglinger O.,Upper Austria University of Applied Sciences | Tollabimazraehno S.,Johannes Kepler University | And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2013

Background/Aims: Acetaminophen (APAP) effects on intestinal barrier properties are less investigated. APAP may lead to a changed bioavailability of a subsequently administered drug or diet in the body. We investigated the influence of APAP on enterocytic cell membrane properties that are able to modify the net intestinal absorption of administered substances across the Caco-2 barrier model. Methods: The effect of APAP on cytotoxicity was measured by LDH assay, TER value and cell capacitance label-free using impedance monitoring, membrane permeability by FITC-dextrans, and efflux transporter MDR1 activity by Rh123. APAP levels were determined by HPLC analysis. Cell membrane topography and microvilli were investigated using SEM and intestinal alkaline phosphatase (Alpi) and tight junction protein 1 (TJP1) expression by western blot analysis. Results: APAP changed the apical cell surface, reduced the number of microvilli and protein expression of Alpi as a brush border marker and TJP1, increased the membrane integrity and concurrently decreased cell capacitance over time. In addition, APAP decreased the permeability to small molecules and increased the efflux transporter activity, MDR1. Conclusion: APAP alters the Caco-2 cell membrane properties by different mechanisms and reduces the permeability to administered substances. These findings may help to optimize therapeutic implications. © 2013 S. Karger AG, Basel.

Rothemund S.,Johannes Kepler University | Aigner T.B.,Johannes Kepler University | Aigner T.B.,Fritsch GmbH | Iturmendi A.,Johannes Kepler University | And 13 more authors.
Macromolecular Bioscience | Year: 2015

Photo-polymerizable scaffolds are designed and prepared via short chain poly(organo) phosphazene building blocks bearing glycine allylester moieties. The polyphosphazene was combined with a trifunctional thiol and divinylester in various ratios, followed by thiol-ene photo-polymerization to obtain porous matrices. Degradation studies under aqueous conditions showed increasing rates in correlation with the polyphosphazene content. Preliminary cell studies show the non-cytotoxic nature of the polymers and their degradation products, as well as the cell adhesion and proliferation of adipose-derived stem cells. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Kianfar E.,Johannes Kepler University | Scha fer C.,Biomedical zet Life Science GmbH | Lornejad-Scha fer M.R.,Biomedical zet Life Science GmbH | Portenkirchner E.,University of Innsbruck | Knor G.,Johannes Kepler University
Inorganica Chimica Acta | Year: 2015

Air- and water-stable rhenium carbonyl complexes are investigated as CO-carriers for studying the light-dependent release of small amounts of carbon monoxide under physiological conditions. The reported very low quantum yields are in a suitable range for studying potential applications as photochemical CO-releasing molecules (Photo-CORMs) without rapidly reaching cytotoxic levels of carbon monoxide. Preliminary results on bio-compatibility and selective uptake of the compounds into human colon carcinoma cells (Caco-2 cell line) are described. © 2015 Elsevier B.V.

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