Biomedical zet Life Science GmbH

Linz, Austria

Biomedical zet Life Science GmbH

Linz, Austria
SEARCH FILTERS
Time filter
Source Type

Patent
Johannes Kepler University and Biomedical Zet Life Science GmbH | Date: 2015-06-16

The invention relates to a polymer for tissue engineering from biodegradable polyphosphazenes, having photopolymerizable side groups, wherein the side groups of the polyphosphazenes are formed exclusively from amino acids and/or amino acid derivatives, which are bonded to the backbone of the polyphosphazene via the amino group of the amino acid and to a spacer attached to the acid group with a carbon chain of length m, which has a vinyl group at the free end, wherein m=0 to m=10.


Patent
Biomedical Zet Life Science GmbH | Date: 2017-06-21

The invention relates to a polymer for tissue engineering composed of biodegradable polyphosphazenes with photopolymerizable side groups, wherein the side groups of the polyphosphazenes are exclusively formed of amino acids and/or amino acid derivatives, wherein said side groups are bound to the backbone of the polyphosphazene via the amino group of the amino acid, and comprising a spacer bound to the acid group having a carbon chain of length m which comprises a vinyl group at its free end, wherein m = 0 to 10.


Kianfar E.,Johannes Kepler University | Scha fer C.,Biomedical zet Life Science GmbH | Lornejad-Scha fer M.R.,Biomedical zet Life Science GmbH | Portenkirchner E.,University of Innsbruck | Knor G.,Johannes Kepler University
Inorganica Chimica Acta | Year: 2015

Air- and water-stable rhenium carbonyl complexes are investigated as CO-carriers for studying the light-dependent release of small amounts of carbon monoxide under physiological conditions. The reported very low quantum yields are in a suitable range for studying potential applications as photochemical CO-releasing molecules (Photo-CORMs) without rapidly reaching cytotoxic levels of carbon monoxide. Preliminary results on bio-compatibility and selective uptake of the compounds into human colon carcinoma cells (Caco-2 cell line) are described. © 2015 Elsevier B.V.


Rothemund S.,Johannes Kepler University | Aigner T.B.,Johannes Kepler University | Aigner T.B.,Fritsch GmbH | Iturmendi A.,Johannes Kepler University | And 13 more authors.
Macromolecular Bioscience | Year: 2015

Photo-polymerizable scaffolds are designed and prepared via short chain poly(organo) phosphazene building blocks bearing glycine allylester moieties. The polyphosphazene was combined with a trifunctional thiol and divinylester in various ratios, followed by thiol-ene photo-polymerization to obtain porous matrices. Degradation studies under aqueous conditions showed increasing rates in correlation with the polyphosphazene content. Preliminary cell studies show the non-cytotoxic nature of the polymers and their degradation products, as well as the cell adhesion and proliferation of adipose-derived stem cells. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Hilber W.,Johannes Kepler University | Lornejad-Schafer M.R.,Biomedical Zet Life Science GmbH | Schafer C.,Biomedical Zet Life Science GmbH | Lederer T.,Johannes Kepler University | And 2 more authors.
Procedia Engineering | Year: 2011

By measuring modulus and phase of either impedance Z or reflection coefficient S 11 of differently shaped electrodes which were in defined contact with a biological sample, we showed that electrical parameters may be utilized to assess biological functions on a three-dimensional (3D) in-vitro system. Using the example of a human hepatic 3D cell model we showed that certain frequency domains in the impedance or reflection coefficient spectra may be assigned to specific biological functions, which would possibly enable the investigation of preclinical drug metabolism and toxicity testing in-vitro. Furthermore, we comment on assets and drawbacks of the electrode designs for the use as a measurement probe in biological studies. © 2011 Published by Elsevier Ltd.


Schafer C.,Biomedical zet Life Science GmbH | Schroder K.R.,Biomedical zet Life Science GmbH | Hoglinger O.,Upper Austria University of Applied Sciences | Tollabimazraehno S.,Johannes Kepler University | And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2013

Background/Aims: Acetaminophen (APAP) effects on intestinal barrier properties are less investigated. APAP may lead to a changed bioavailability of a subsequently administered drug or diet in the body. We investigated the influence of APAP on enterocytic cell membrane properties that are able to modify the net intestinal absorption of administered substances across the Caco-2 barrier model. Methods: The effect of APAP on cytotoxicity was measured by LDH assay, TER value and cell capacitance label-free using impedance monitoring, membrane permeability by FITC-dextrans, and efflux transporter MDR1 activity by Rh123. APAP levels were determined by HPLC analysis. Cell membrane topography and microvilli were investigated using SEM and intestinal alkaline phosphatase (Alpi) and tight junction protein 1 (TJP1) expression by western blot analysis. Results: APAP changed the apical cell surface, reduced the number of microvilli and protein expression of Alpi as a brush border marker and TJP1, increased the membrane integrity and concurrently decreased cell capacitance over time. In addition, APAP decreased the permeability to small molecules and increased the efflux transporter activity, MDR1. Conclusion: APAP alters the Caco-2 cell membrane properties by different mechanisms and reduces the permeability to administered substances. These findings may help to optimize therapeutic implications. © 2013 S. Karger AG, Basel.


Clara S.,Johannes Kepler University | Lornejad-Schafer M.R.,Biomedical zet Life Science GmbH | Schafer C.,Biomedical zet Life Science GmbH | Jakoby B.,Johannes Kepler University | Hilber W.,Johannes Kepler University
Procedia Engineering | Year: 2014

We present a feasibility study aiming at the detection of silica nanoparticles (NPs) in human intestinal epithelial cells. Caco-2 cells were maintained in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) and differentiated for 21 days. To study the effect of silica NPs on the differentiated Caco-2 cell, cells were treated for 24h with different doses. We measured the serum-free culture medium first, which was supplemented with different doses of silica NPs using a parallel-plate capacitor principle to demonstrate the sensitivity of the sensing principle to the particle concentration. In a next step we treated Caco-2 cells with serum-free culture medium containing silica NPs. After 24h incubation time, culture medium was removed and cells were washed with PBS, so that topical adherent NPs should have been removed. We finally present the use of a second coplanar electrode design with eight single electrodes connectable in arbitrary combination to detect the silica NPs in cells. © 2014 The Authors. Published by Elsevier Ltd.


Stadlbauer V.,Upper Austria University of Applied Sciences | Haselgrubler R.,Upper Austria University of Applied Sciences | Lanzerstorfer P.,Upper Austria University of Applied Sciences | Plochberger B.,Upper Austria University of Applied Sciences | And 7 more authors.
PLoS ONE | Year: 2016

Induction of GLUT4 translocation in the absence of insulin is considered a key concept to decrease elevated blood glucose levels in diabetics. Due to the lack of pharmaceuticals that specifically increase the uptake of glucose from the blood circuit, application of natural compounds might be an alternative strategy. However, the effects and mechanisms of action remain unknown for many of those substances. For this study we investigated extracts prepared from seven different plants, which have been reported to exhibit anti-diabetic effects, for their GLUT4 translocation inducing properties. Quantitation of GLUT4 translocation was determined by total internal reflection fluorescence (TIRF) microscopy in insulin sensitive CHO-K1 cells and adipocytes. Two extracts prepared from purslane (Portulaca oleracea) and tindora (Coccinia grandis) were found to induce GLUT4 translocation, accompanied by an increase of intracellular glucose concentrations. Our results indicate that the PI3K pathway is mainly responsible for the respective translocation process. Atomic force microscopy was used to prove complete plasma membrane insertion. Furthermore, this approach suggested a compound mediated distribution of GLUT4 molecules in the plasma membrane similar to insulin stimulated conditions. Utilizing a fluorescent actin marker, TIRF measurements indicated an impact of purslane and tindora on actin remodeling as observed in insulin treated cells. Finally, in-ovo experiments suggested a significant reduction of blood glucose levels under tindora and purslane treated conditions in a living organism. In conclusion, this study confirms the anti-diabetic properties of tindora and purslane, which stimulate GLUT4 translocation in an insulin-like manner. © 2016 Stadlbauer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Witteveen W.,Upper Austria University of Applied Sciences | Wagner C.,Upper Austria University of Applied Sciences | Jachs P.,Biomedical zet Life Science GmbH | Froschauer S.,Biomedical zet Life Science GmbH | Schoffl H.,Biomedical zet Life Science GmbH
Conference Proceedings of the Society for Experimental Mechanics Series | Year: 2015

A suspicion of a femoral neck fracture is a frequently recurring situation, especially in nursing homes. For the clarification of such a suspicion normally imaging techniques are used. Such equipment is expensive and therefore is located in hospitals. In addition to the costs, a transport causes stress for the patient. This pilot study is devoted to the question whether the detection of a femoral neck fracture with vibration measurements is possible in principal. For this purpose vibration measurements on a dead body with intact, with partially fractured and with complete cut femoral neck have been performed. The frequency response function has been determined for all combinations on both sides of the body. It turned out that there is a difference in the frequency response functions of the fractured bone with respect to the intact bone when shaker testing is used. © The Society for Experimental Mechanics, Inc. 2015.


PubMed | Biomedical zet Life Science GmbH and Upper Austria University of Applied Sciences
Type: Journal Article | Journal: PloS one | Year: 2016

Induction of GLUT4 translocation in the absence of insulin is considered a key concept to decrease elevated blood glucose levels in diabetics. Due to the lack of pharmaceuticals that specifically increase the uptake of glucose from the blood circuit, application of natural compounds might be an alternative strategy. However, the effects and mechanisms of action remain unknown for many of those substances. For this study we investigated extracts prepared from seven different plants, which have been reported to exhibit anti-diabetic effects, for their GLUT4 translocation inducing properties. Quantitation of GLUT4 translocation was determined by total internal reflection fluorescence (TIRF) microscopy in insulin sensitive CHO-K1 cells and adipocytes. Two extracts prepared from purslane (Portulaca oleracea) and tindora (Coccinia grandis) were found to induce GLUT4 translocation, accompanied by an increase of intracellular glucose concentrations. Our results indicate that the PI3K pathway is mainly responsible for the respective translocation process. Atomic force microscopy was used to prove complete plasma membrane insertion. Furthermore, this approach suggested a compound mediated distribution of GLUT4 molecules in the plasma membrane similar to insulin stimulated conditions. Utilizing a fluorescent actin marker, TIRF measurements indicated an impact of purslane and tindora on actin remodeling as observed in insulin treated cells. Finally, in-ovo experiments suggested a significant reduction of blood glucose levels under tindora and purslane treated conditions in a living organism. In conclusion, this study confirms the anti-diabetic properties of tindora and purslane, which stimulate GLUT4 translocation in an insulin-like manner.

Loading Biomedical zet Life Science GmbH collaborators
Loading Biomedical zet Life Science GmbH collaborators