Gutierrez-Gonzalez A.,Metropolitan Autonomous University |
Gutierrez-Gonzalez A.,Biomedical Research Institute of Madrid |
Belda-Iniesta C.,Ghm Grupo Hospital Of Madrid |
Belda-Iniesta C.,Hospital Universitario La Paz |
And 9 more authors.
Apoptosis | Year: 2013
Our results demonstrate that the addition of cisplatin after paclitaxel-induced mitotic arrest was more effective than individual treatment on gastric adenocarcinoma cells (MKN45). However, the treatment did not induce benefits in cells derived from lymph node metastasis (ST2957). Time-lapse microscopy revealed that cell death was caused by mitotic catastrophe and apoptosis induction, as the use of the caspase inhibitor z-VAD-fmk decreased cell death. We propose that the molecular mechanism mediating this cell fate is a slippage suffered by these cells, given that our Western blot (WB) analysis revealed premature cyclin B degradation. This resulted in the cell exiting from mitosis without undergoing DNA damage repair, as demonstrated by the strong phosphorylation of H2AX. A comet assay indicated that DNA repair was impaired, and Western blotting showed that the Chk2 protein was degraded after sequential treatment (paclitaxel-cisplatin). Based on these results, the modulation of cell death during mitosis may be an effective strategy for gastric cancer therapy. © 2012 Springer Science+Business Media New York.