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Colomer E.G.,University of Valencia | Colomer E.G.,Biomedical Research in the Valencian Community FISABIO | Gomez M.A.M.,University of Valencia | Gomez M.A.M.,Biomedical Research in the Valencian Community FISABIO | And 6 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2014

The main objective of our study is to develop a simple, fast and reliable method for measuring β-glucocerebrosidase activity in Gaucher patients leukocytes in clinical practice. This measurement may be a useful marker to drive dose selection and early clinical decision making of enzyme replacement therapy. We measure the enzyme activity by high-performance liquid chromatography with ultraviolet detection and 4-nitrophenyl-β- d-glucopyranoside as substrate.A cohort of eight Gaucher patients treated with enzyme replacement therapy and ten healthy controls were tested; median enzyme activity values was 20.57. mU/ml (interquartile range 19.92-21.53. mU/ml) in patients and mean was 24.73. mU/ml (24.12-25.34. mU/ml) in the reference group, which allowed the establishment of the normal range of β-glucocerebrosidase activity. The proposed method for leukocytes glucocerebrosidase activity measuring is fast, easy to use, inexpensive and reliable. Furthermore, significant differences between both populations were observed (p= 0.008). This suggests that discerning between patients and healthy individuals and providing an approach to enzyme dosage optimization is feasible. This method could be considered as a decision support tool for clinical monitoring. Our study is a first approach to in depth analysis of enzyme replacement therapy and optimization of dosing therapies. © 2013 Elsevier B.V.

Alis R.,University of Valencia | Sanchis-Gomar F.,University of Valencia | Sanchis-Gomar F.,Institute of Health Research INCLIVA | Pareja-Galeano H.,University of Valencia | And 11 more authors.
Clinical Biochemistry | Year: 2014

Objectives: The aim of study was to explore whether a relationship exists between homocysteine and irisin in type 2 diabetes (T2D) patients-a population with a high risk of developing cardiovascular disease-and euglycemic controls. Design and methods: 69 T2D patients and 75 control subjects (adjusted by body mass index (BMI)) were included in the study. Irisin and homocysteine concentrations and anthropometric and biochemical variables were determined. Results: Levels of homocysteine were significantly higher (11.0 ± 3.0 vs 12.4 ± 4.2. μmol/l) and levels of irisin were lower (279 ± 58 vs 263 ± 38. ng/ml) in T2D patients. When both T2D and controls were considered, irisin was found to correlate only with homocysteine (r = - 0.215; p = 0.011). Moreover, a decreasing trend in irisin levels was observed according to homocysteine tertile (p = 0.034). Conclusions: Our results provide evidence of an association between irisin and homocysteine, which may be due to nicotinamide metabolism. The clinical significance of this relationship is unclear, but our findings may prompt further mechanistic research to investigate the role played by irisin in vascular disorders. © 2014 The Canadian Society of Clinical Chemists.

Hernandez-Mijares A.,Biomedical Research in the Valencian Community FISABIO | Hernandez-Mijares A.,University of Valencia | Hernandez-Mijares A.,Institute of Health Research INCLIVA | Banuls C.,Biomedical Research in the Valencian Community FISABIO | And 14 more authors.
Journal of Functional Foods | Year: 2015

The anti-diabetic properties of an inositol-enriched beverage (IEB) on the endothelial function and redox status in diabetic subjects were assessed. This was a 12-week, double-blind randomized controlled trial employing thirty-eight diabetic subjects that were divided into two intervention groups: one receiving an IEB and the other a sucrose-enriched beverage. Subjects consuming IEB exhibited a significant decrease in triacylglycerol (8.82%) and HbA1c (4.53%) levels. Continuous glucose monitoring revealed a significant net reduction of 2.51 and 7.11% during postprandial and overnight fasting periods after consumption of IEB, respectively. Moreover, IEB improved endothelial function by reducing P-selectin levels and leucocyte-endothelium interactions, as there was an increase in rolling velocity and a reduction in polymorphonuclear leucocyte adhesion and induced a significant diminution in the generation of ROS. The present results show that IEB supplementation induces a significant improvement in glycaemic control in diabetic subjects by improving endothelial function and intracellular redox status. © 2015 Elsevier Ltd.

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