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Nomani A.,Tehran University of Medical Sciences | Nomani A.,Zanjan University of Medical Sciences | Haririan I.,Tehran University of Medical Sciences | Haririan I.,Biomaterials Research Center | And 6 more authors.
International Journal of Nanomedicine | Year: 2010

To gain a deeper understanding of the physicochemical phenomenon of self-assembled nanoparticles of different generations and ratios of poly (amidoamine) dendrimer (PAMAM) dendrimer and a short-stranded DNA (antisense oligonucleotide), multiple methods were used to characterize these nanoparticles including photon correlation spectroscopy (PCS); zeta potential measurement; and atomic force microscopy (AFM). PCS and AFM results revealed that, in contrast to larger molecules of DNA, smaller molecules produce more heterodisperse and large nanoparticles when they are condensed with a cationic dendrimer. AFM images also showed that such nanoparticles were spherical. The stability of the antisense content of the nanoparticles was investigated over different charge ratios using polyacrylamide gel electrophoresis. It was clear from such analyses that much more than charge neutrality point was required to obtain stable nanoparticles. For cell uptake, self-assembled nanoparticles were prepared with PAMAM G5 and 5'-FITC labeled antisense and the uptake experiment was carried out in T47D cell culture. This investigation also shows that the cytotoxicity of the nanoparticles was dependent upon the generation and charge ratio of the PAMAM dendrimer, and the antisense concentration had no significant effect on the cytotoxicity. © 2010 Nomani et al, publisher and licensee Dove Medical Press Ltd.


Haririan I.,Biomaterials Research Center | Ghane Z.Z.,Tehran University of Medical Sciences | Namazi H.,University of Tabriz
International Journal of Nanomedicine | Year: 2010

Due to their unique properties, Anticancer dendrimer-based drugs have been displaying promising results in both in vitro and in vivo in the treatment of cancerous cells, as compared to the traditional polymers. In this report, two conjugates (G1+Pt and G2+Pt) of cisplatin [cis-diaminedichloroplatinum; (CDDP)] with two generations (G1, G2) of a biocompatible anionic dendrimer were prepared in an aqueous media. Their potential cytotoxic effects, in two sensitive cancer cell lines HT1080 and CT26 together with one resistant cancer cell line SKOV3, using MTT (methyl thiazolyl tetrazolium) assay were examined. Hemolytic impacts and cell death mechanisms of the conjugates on human blood and HT1080 cell line were also investigated. The conjugate G2+Pt showed greater toxicity up to 9× and 2× in the sensitive and resistant cell lines (IC50 comparison, inhibitory concentration) respectively when compared to the parent drug. The G1+Pt conjugate showed greater toxicity only in the sensi- tive HT1080 (2×) and CT26 (3.7×) cell lines. Moreover, the G1+Pt conjugate was less toxic approximately one third of the cisplatin in SKOV3 after 48 hrs of incubation. In summary, the G2+Pt conjugate had greater toxicity than the G1+Pt conjugate and cisplatin, based on the in vitro results. Approximately the same hemolysis behavior was observed for both conjugates and cisplatin. Both apoptosis and necrosis mechanisms (about 2× more than cisplatin) were attributed to conjugates and cisplatin in a direct correlation between the concentration and the degree of cell death. In conclusion, these conjugates with such high potency and minimum hemolysis would be suitable candidates for use against these cancerous cell lines as efficient and novel antitumor agents. © 2010 Haririan et al.


Azizi E.,Tehran University of Medical Sciences | Namazi A.,Tehran University of Medical Sciences | Haririan I.,Tehran University of Medical Sciences | Haririan I.,Biomaterials Research Center | And 6 more authors.
International Journal of Nanomedicine | Year: 2010

Chitosan/alginate nanoparticles which had been optimized in our previous study using two different N/P ratios were chosen and their ability to release epidermal growth factor receptor (EGFR) antisense was investigated. In addition, the stability of these nanoparticles in aqueous medium and after freeze-drying was investigated. In the case of both N/P ratios (5, 25), nanoparticles started releasing EGFR antisense as soon as they were exposed to the medium and the release lasted for approximately 50 hours. Nanoparticle size, shape, zeta potential, and release profile did not show any significant change after the freeze-drying process (followed by reswelling). The nanoparticles were reswellable again after freeze-drying in phosphate buffer with a pH of 7.4 over a period of six hours. Agarose gel electrophoresis of the nanoparticles with the two different N/P ratios showed that these nanoparticles could protect EGFR antisense molecules for six hours. © 2010 Azizi et al, publisher and licensee Dove Medical Press Ltd.


Parsapour A.,Biomaterials Research Center | Khorasani S.N.,Isfahan University of Technology | Fathi M.H.,Isfahan University of Technology
Acta Metallurgica Sinica (English Letters) | Year: 2013

The aim of this studies at simultaneous improvement of the corrosion behavior and biocompatibility of metallic implant and bone Osseointegration simultaneously. Stainless steel 316L (SS) was used as metallic substrate and after surface treatment with 15 vol.% sulfuric acid, it was coated with hydroxyapatite coating employing plasma - spraying process. Structure characterization techniques including XRD, SEM and EDX were also utilized to investigate the microstructure, morphology, and crystallinity of the coating. Electrochemical potentiodynamic tests were performed in two types of physiological solutions in order to determine and compare the corrosion resistance behavior of the coated and uncoated specimens as an indication of biocompatibility. The results indicate that the surface treatment and hydroxyapatite coating improve the corrosion resistance behavior of SS. The corrosion current density of the surface treated and the hydroxyapatite coated SS also decrease. These also show that surface treated and hydroxyapatite coated SS can be used as human body implants with the goals of corrosion resistance improvement (biocompatibility) and bone osseointegration. © The Chinese Society for Metals and Springer-Verlag Berlin Heidelberg.


Jung H.,Biomaterials Research Center | Kim H.H.,Yonsei University | Lee D.H.,Biomaterials Research Center | Hwang Y.-S.,Kyung Hee University | And 2 more authors.
Cytotechnology | Year: 2011

Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). However, the main soluble factor of ADSC-CM which mediates paracrine effects and its underlying mechanism has not been elucidated yet. In this study, we considered transforming growth factor-beta1 (TGF-β1) as a strong candidate for paracrine effect of ADSC-CM and investigated collagen synthesis and hyaluronic acid synthase (HAS) expression. After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. Furthermore, to clarify effects of TGF-β1 as a paracrine mediator, TGF-β1 antibody and external supplementary TGF-β1 were treated to HDFs. Collagens type I, type III, HAS-1 and HAS-2 mRNA expressions of HDFs were greatly increased by ADSC-CM treatment, however there was no change in TGF-β1 antibody treated HDFs compared with non-treated control. These results strongly demonstrate that TGF-β1 plays an important role as a paracrine mediator of ECM synthesis. The fact that TGF-β1 contained in ADSC-CM not only accelerates collagen deposition but also increase hyaluronic acid synthesis of HDFs through HAS-1 and HAS-2 expression was also elucidated in this study. Therefore, ADSC-CM shows promise for the treatment of cutaneous wounds and accelerates granulation formation during healing process. © 2010 Springer Science+Business Media B.V.

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