Chen T.,BiologyChinese Academy of science |
Li H.-M.,BiologyChinese Academy of science |
Li H.-M.,University of Chinese Academy of Sciences |
Zou D.-L.,BiologyChinese Academy of science |
And 4 more authors.
Journal of Separation Science | Year: 2014
Barley seedlings are rich in flavones that can have positive effects on people with antihypoxia and antifatigue. Lutonarin and saponarin are two major flavonoid glycosides that have unique structures in barley seedlings. This study presents a new approach for the preparation of lutonarin and saponarin from barely seedlings by membrane separation technology and preparative high-performance liquid chromatography. Preparative conditions of these two flavonoid glycosides by membrane separation technology were studied using response surface methodology. Under the optimized conditions, the total contents of these two flavonoid glycosides amounts to 17.0%. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.