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Junhua L.,Biology Institute of Hebei Academy of science | Chunsheng L.,Biology Institute of Hebei Academy of science | Meng W.,Biology Institute of Hebei Academy of science | Yan Z.,Hebei Food Inspection and Research Institute | And 3 more authors.
Journal of Food Science | Year: 2015

In this study a one-step immunochromatographic assay based on competitive format was developed for the rapid detection of phenylethanolamine A (PEAA) residues in urine and pork samples. A monoclonal antibody against PEAA was produced from BALB/c mice immunized with the PEAA-BSA conjugate. The results of this qualitative test strip were to be interpreted visually. The visual detection limit (VDL) and threshold level of the optimized immunochromatographic assay for PEAA were 0.1 ng/mL and 0.5 ng/mL, respectively. Cross-reactions with other β-agonists were not significant inhibitions to the performance of the test strip assay. The results from the test strip were in a good agreement with those obtained using a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay. The immunochromatographic assay developed here was a useful on-site screening tool that is rapid to use, low in cost, and extremely convenient for the detection of PEAA in urine samples and pork samples. Practical Application: Phenylethanolamine A (PEAA) is one of the β-agonists, which has been used not only to treat diseases, but also to promote growth in animals. However, concerning the harm to function of human body, a new fast and convenient method for monitoring PEAA should be established. We describe the development of a new test assay for rapid detection of PEAA in urine and pork samples. © 2015 Institute of Food Technologists®.


PubMed | Biology Institute of Hebei Academy of science
Type: Evaluation Studies | Journal: Journal of food science | Year: 2015

In this study a one-step immunochromatographic assay based on competitive format was developed for the rapid detection of phenylethanolamine A (PEAA) residues in urine and pork samples. A monoclonal antibody against PEAA was produced from BALB/c mice immunized with the PEAA-BSA conjugate. The results of this qualitative test strip were to be interpreted visually. The visual detection limit (VDL) and threshold level of the optimized immunochromatographic assay for PEAA were 0.1 ng/mL and 0.5 ng/mL, respectively. Cross-reactions with other -agonists were not significant inhibitions to the performance of the test strip assay. The results from the test strip were in a good agreement with those obtained using a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay. The immunochromatographic assay developed here was a useful on-site screening tool that is rapid to use, low in cost, and extremely convenient for the detection of PEAA in urine samples and pork samples.

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