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Chinchiná, Colombia

Valido D.P.,Odontologa | Valido D.P.,Tiradentes University | Bastos T.S.,Academica de Biomedicina | De Albuquerque-Junior R.L.C.,Odontologa | And 8 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2010

Introduction; Otoliths are calcareous concrescences present in the inner ear of fishes. Since they are rich in minerals considered essential to the bone mineralization process on a protein matrix (otolin), it has been suggested that otoliths may work as biominerals. Objective; The objective in this study was to analyze the regeneration capacity of bone defects treated with sterile gelatinous otolith preparation. Material and method; The experiment was performed with 20 Wistar rats randomized into two groups, in which a bone defect was inflicted in the tibia. In group 1 (experimental), bone cavities were filled with otoliths. In group 2 (control), bone cavities did not receive any additional treatment. The animals were sacrificed 14 days after surgical procedures. The tibias were removed, fixed in formalin at 10%, decalcified in nitric acid at 5% and histologically processed. Before sacrifice, blood samples were collected to evaluate serum dosage of calcium (Ca2+) and alkaline phosphatase (Alkp). Results; Histological sections revealed that the newly formed trabecular bone was denser and the periosteal reabsorptive activity was less conspicuous in the experimental group. However, there was no statistically significant difference in the area of bone formation between G1 (1.9 ± 0.3 mm2) and G2 (1.5 ± 0.4 mm2) (p = 0.0617). Biochemical (Ca2+ and Alkp) parameters were within normal range. Discussion/conclusion: These data suggest that otoliths may be an adjuvant to the dynamics of bone regeneration.

This work analyzes the interactions between the community of Japuí Quarter, in the city of São Vicente (SP), and Xixová-Japuí (PEXJ) State Park. For doing this, a semi-structured questionnaire was applied to inhabitants of the quarter and actors related to the Park, including its manager at the time, approaching aspects on environmental information, interaction with the UC (Unit of Conservation) territory, knowledge and availability of information on the Park. The results showed a low middle class population with low degree of instruction in general. The relationship of the community with PEXJ management was almost nonexistent, something which may result from the fact the population are not considered by the UC. One also proved the low knowledge and interest on the Park by inhabitants, as well as the failure of the administration, until that moment, in inserting the community in the unit's discussion and management. Such situation allowed the establishment of a conflicting relationship between the PEXJ and members of the community, leading to a situation where management has not established participative actions.

De Sousa V.A.,Embrapa Florestas | Schemberg E.A.,Biologo | Aguiar A.V.,Embrapa Florestas
Scientia Forestalis/Forest Sciences | Year: 2010

Syagrus romanzoffiana (jerivá) is a native palm species from Brazil with potential as ornamental, for apicultural and for the maintenance of the wild fauna, because it produces nutritious and abundant fruits. As a consequence of the indiscriminate native forests exploitation and due to its ecological importance in the natural environment, measures related to its conservation are needed. Pollen storage can play an important tool not only for ex situ conservation but also for tree breeding programs. The knowledge to evaluate its pollen viability is lacking. The present work aimed at identifying adequate culture media for jerivá pollen germination, taking into consideration different nutrient concentration (B, Ca, Mg and K) within the Brewbaker and Kwack medium modified for Ca, B, sucrose (100 g.L-1) and agar (3 g.L-1 ). Germination percentages (%) were submitted to variance analysis and the average compared by the Tukey test at 1 % level. According to this study, nutrient addition did not increase significantly in vitro germination. Therefore the medium composed only by sucrose and agar is indicated to evaluate Syagrus romanzoffiana pollen viability.

Beruski G.C.,University of Sao Paulo | Pereira A.B.,Engenheiro Agronomo | Jaccoud-Filho D.S.,Biologo | Sartori F.F.,University of Sao Paulo | Sentelhas P.C.,University of Sao Paulo
Bioscience Journal | Year: 2015

The Sclerotinia sclerotiorum (Lib.) de Bary fungus is a necrotrophic and polyphagous pathogen with soybean crop as one of its most important hosts. However in order to occur epidemics caused by such a pathogen it is necessary that the climatic conditions, mainly air temperature and relative humidity be favorable to the occurrence and development of the disease. The current research aimed to assess the incidence and severity of S. sclerotiorum in soybean plants grown in Arapoti, PR, Brazil, as a function of different plant populations, row spacing, and microclimate. The experiment was conducted in a naturally infested area. The experimental design adopted was a randomized block in a factorial combination with 4 row spacing (0.35, 0.45, 0.60, 0.75 m) and 4 plant populations (150, 200, 250; 300 thousand plants per hectare), totaling 16 treatments and 4 replications. Throughout the current study we performed 4 assessments of incidence and severity. Local climatic conditions were favorable for the incidence and severity of the pathogen. Mean air temperature, rainfall, relative humidity and leaf wetness duration, monitored during all experimental period, favored the development of the pathogen in the experimental field. Yield and 100 seed weight did not show fluctuations as a function of variations on the incidence and severity of white mold in soybean crop fields. Nevertheless, crop yield was higher under a reduced spacing, whereas the 100 seed weight showed the highest values under the lowest plant population treatment. © 2015, Universidade Federal de Uberlandia.

Einloft R.,Federal University of Vicosa | Ruiz H.A.,Federal University of Espirito Santo | da Costa L.M.,Federal University of Vicosa | Schaefer C.E.G.R.,Federal University of Vicosa | And 3 more authors.
Revista Brasileira de Ciencia do Solo | Year: 2013

The succession of events after revegetation has rarely been studied. A plant consortium with a good initial development may come to be inadequate later, resulting in exposed soil, susceptible to the weathering forces. The objective of this study was to monitor characteristics associated with vegetative stabilization and, in the long term, the appearance of new cover forms or soil exposure in response to planting grasses and legumes on a steep road slope alongside a highway. After four years of recovery, new forms of soil cover or exposure were observed in the experimental plots, called typologies, described below: brachiaria grass cover, legume plants, gordura grass, invasive species, geotextile, decomposing residues, microphytic crusts, soil crust, exposed soil, erosion, and rock outcrops. The characteristics of these typologies were quantified by two surveys, before and after the rainy season. In addition, the different typologies of each experimental plot were mapped; these maps were used to analyze the dynamics, spatial distribution, frequency, and competition among typologies identified in the two surveys. Of the total 11 typologies, the vegetation species and microphytic crusts were the most relevant for revegetation. Microphytic crusts were very important in the initial stage of ecological succession, resulting in rapid stabilization and reclamation of degraded surfaces and favoring the appearance of invasive species. The seasonal variation between the two surveys showed that erosion and soil exposure decreased with increase in vegetation cover and microphytic crust development.

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