Biological Research Division

Dhaka, Bangladesh

Biological Research Division

Dhaka, Bangladesh
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Kamal A.K.I.,Jahangirnagar University | Islam M.R.,Jahangirnagar University | Islam M.R.,Gas and Mineral Corporation PETROBANGLA | Hassan M.,Jahangirnagar University | And 3 more authors.
Environmental Processes | Year: 2016

The presence of trace metals in landfill soil and plants pose a risk to the environment and human health. This study was conducted to determine trace metal concentrations in soil and different plant parts that grow in the Amin Bazar landfill, Bangladesh, to evaluate the possible human health risk on consumer. The collected soil and plant samples were analyzed for trace metals using a Flame Atomic Absorption Spectrophotometer. The mean concentrations of trace metal found in the soil were in the following order: zinc (Zn) > manganese (Mn) > lead (Pb) > copper (Cu) > chromium (Cr) > nickel (Ni). Considering all selected plant species, the mean concentrations of trace metals were in the following order: Zn > Ni > Mn > Cu. On the other hand considering all measured trace metals, the accumulation trend in plant species were in the following order: Carica papaya > Enhydra flactuans > Amaranthus gangeticus > Ipomoea aquatica > Sesbania cannabina > Musa sapientum. Pb and Cr were not accumulated in the studied plant species. Translocation factor (TF), bioaccumulation factor (BAF) and bioaccumulation coefficient (BAC) were calculated for the assessment of mobility of trace metals from root to shoot, soil to shoot and soil to whole plant, respectively. TF values showed that the plant species effectively translocate trace metals from roots to the shoots, suggesting that they are suitable for phytoextraction. According to BAF all studied plants were excluders for all metals except Ni, and according to BAC, all studied plants were hyperaccumulators of Ni. The daily metal intake and health risk index values of the studied metals, except of Ni, indicated that there is a relative absence of health risks associated with the ingestion of contaminated edible parts of plants. © 2016, Springer International Publishing Switzerland.

Sinha P.,Biological Research Division | Jahan M.A.A.,Biological Research Division
Plant Tissue Culture and Biotechnology | Year: 2012

For high frequency regeneration of Rhyncnostylis retusa (Lin.) Blume apical nodal segments were used. Half strength MS + 2% sucrose + 1.5 mg/l BA + 0.5 mg/l NAA + 2 g/l peptone + 10% (v/v) coconut water (CW) + 0.5 g/l activated charcoal (AC) was the best nutrient medium, on which 89% cultures induced 8 microshoots per culture. Subculture of microshoots for further 8 weeks on the same nutrient medium enhanced the number of microshoots up to 95. For further proliferation of microshoots, their development into shoots as well as formation of secondary microshoots from the base of the old ones, the best medium was half strength of MS + 2% sucrose + 2 g/l peptone + 10% (v/v) CW + 0.5 g/l AC + 150 mg/l L-glutamine. Plantlets with roots were obtained in half strength of MS + 2% sucrose + 2 g/l peptone + 10% (v/v) CW + 0.5 g/l AC + 5.0 g/l banana powder, on which cent per cent shoots rooted within eight weeks. The pH of all the categories of cultures were maintained at 5.6 before adding 2.2 g/l gelrite and autoclaving, and the cultures were incubated at 2000 - 3000 lux for 16/8 hrs light/dark at 24 ± 2oC. Regeneration of plantlets continued due to repeated subculture of microshoots and regenerants were acclimatized and established in the nursery.

Sinha P.,Biological Research Division | Jahan M.A.A.,Biological Research Division | Munshi J.L.,Biological Research Division | Khatun R.,Biological Research Division
Plant Tissue Culture and Biotechnology | Year: 2010

Young leaf explants from mature plant of Phalaenopsis amabilis cv. Lovely were cultured on half strength of MS supplemented with BA (2.0 mg/l), NAA (0.5 mg/l), 2% (w/v) sucrose, 10% (v/v) coconut water, 2 g/l peptone and 1 g/l activated charcoal. Each section of explant produced ten protocorm-like bodies (PLBs) after 12 weeks of culture. The PLBs were subcultured on the same nutrient medium but without phytohormone and addition of 150 mg/l L-glutamine, where PLBs were found to be enlarged with leafy shoots and new PLBs were induced from the base of the old ones. Plantlet development from leafy shoots was achieved on half strength MS supplemented with 2 g/l peptone, 2% (w/v) sucrose, 10% (v/v) CW and 1 g/l activated charcoal, where 100% explants were developed into plantlets with roots within eight weeks. The addition of 2.5 g/l banana powder enhanced the number and length of roots. Within the first 32 weeks after initiation of culture about 1200 plantlets as well as a huge amount of PLBs were achieved from a single explant section. The plantlets were acclimated in natural environment.

Hassan M.,Jahangirnagar University | Tanvir Rahman M.A.T.M.,Jahangirnagar University | Saha B.,Biological Research Division | Kamal A.K.I.,Jahangirnagar University
American Journal of Environmental Sciences | Year: 2015

The pollution of river water and sediments by heavy metals has assumed serious problems due to their toxicity and accumulative behavior. The present study has been undertaken to assess the levels of heavy metals and the extent of pollution in the surface water and sediments from the Meghna river. Water and sediment samples were collected by the Standard Methods and, processed and analyzed for heavy metals using Flame Atomic Absorption Spectrophotometer (FAAS). The mean concentrations of heavy metal found in the river water were in the order of: Fe (1.0224 mg L-1) > Zn (0.0364 mg L-1) > Cr (0.0346 mg L-1) > Mn (0.0088 mg L-1) > Cd (0.003 mg L-1) and in the sediments in the order of: Fe (1281.416 mg kg-1) > Mn (442.596 mg kg-1) > Zn (79.021 mg kg-1) > Ni (76.116 mg kg-1) > Cr (31.739 mg kg-1) > Pb (9.4702 mg kg-1) > Cd (0.230 mg kg-1). Pb and Ni were found below detection limit in river water. Based on the findings, the Meghna river water can be considered as unpolluted with respect to Cd, Cr, Mn and Zn, whereas concentration of Fe was above the standard value according to recommended standard guidelines. According to Sediment Quality Guideline (USEPA, 1989), sediments were not polluted for Cd, Pb and Zn; moderately polluted for Cr and Mn and heavily polluted for Ni. The sediment geo-accumulation index (Igeo) values showed no pollution for most of sampling sites for all studied heavy metals. Pollution Load Index (PLI) values showed that all the studied sampling sites were not polluted and on the other hand mean Contamination Factor (CF) values showed low pollution for all measured heavy metals except Ni which indicated moderate pollution. This study can be used as reference to monitor the quality of water and sediments of the Meghna river. © 2015 Mahmud Hassan, Mirza A.T.M. Tanvir Rahman, Badhan Saha and Abdul Kadir Ibne Kamal.

Shahariar M.S.,Biological Research Division | Tahsin S.,Biological Research Division | Gani M.N.,Bangladesh Jute Research Institute BJRI | Huq S.M.I.,University of Dhaka
Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences | Year: 2012

The growth and biomass productivity of kenaf (Hibiscus cannabinus L.) grown with different sources of organic waste viz. sewage sludge, poultry litter, cow dung and rice straw application were observed in a field experiment. Organic wastes were applied at the rate of 5 t/ha and were compared with recommended dose of fertilizers and control. The plants were harvested at 120 days after sowing (at the flowering stage). Different sources of organic wastes had a significant effect (P < 0.05) on the growth indices and biomass productivity of kenaf. Increased plant growth and biomass productivity were in the order of sewage sludge > cow dung>poultry litter > rice straw treatments. Among the four sources of organic wastes, sewage sludge treated plot produced the highest mean biomass of 23.33 t/ha (dry weight basis) which was 14.64% higher than the mean biomass production from control plot.

Matsui M.S.,Biological Research Division | Petris M.J.,University of Missouri | Karaman-Jurukovska N.,Biological Research Division | Muizzuddin N.,Biological Research Division | And 2 more authors.
Journal of Investigative Dermatology | Year: 2015

Omeprazole is a proton pump inhibitor used in the treatment of peptic ulcer disease and gastrosophageal reflux disease and acts by irreversibly blocking ATP4A, a P-type H+/K+ ATPase in gastric parietal cells. We found that omeprazole and its closely related congeners inhibited melanogenesis at micromolar concentrations in B16 mouse melanoma cells, normal human epidermal melanocytes, and in a reconstructed human skin model. Omeprazole topically applied to the skin of UV-irradiated human subjects significantly reduced pigment levels after 3 weeks compared with untreated controls. Omeprazole had no significant inhibitory effect on the activities of purified human tyrosinase or on the mRNA levels of tyrosinase, dopachrome tautomerase, Pmel17, or MITF mRNA levels. Although melanocytes do not express ATP4A, they do express ATP7A, a copper transporting P-type ATPase in the trans-Golgi network that is required for copper acquisition by tyrosinase. ATP7A relocalization from the trans-Golgi network to the plasma membrane in response to elevated copper concentrations in melanocytes was inhibited by omeprazole. Omeprazole treatment increased the proportion of EndoH sensitive tyrosinase, indicating that tyrosinase maturation was impaired. In addition, omeprazole reduced tyrosinase protein abundance in the presence of cycloheximide, suggestive of increased degradation. Our findings are consistent with the hypothesis that omeprazole reduces melanogenesis by inhibiting ATP7A and by enhancing degradation of tyrosinase. © 2015 The Society for Investigative Dermatology.

Hassan S.K.M.,Biological Research Division | Khatun R.,Biological Research Division
Bangladesh Journal of Botany | Year: 2010

Shoot tips and nodal explants from in vitro growing seedlings of Ficus glomerata Roxb. (Moraceae). showed best shoot induction (88%) on MS medium supplemented with 0.5 mg/1 BAP, where maximum number of shoots were produced per culture. In vitro raised shoots rooted well on half strength of MS medium with 2.0 mg/1 IB A + 0.1 mg/1 NAA. The survival rate of regenerated plantlets was 82%.

Sayeed Hassan A.K.M.,Biological Research Division | Begum N.,Biological Research Division | Sultana R.,Biological Research Division | Khatun R.,Biological Research Division
Plant Tissue Culture and Biotechnology | Year: 2011

An efficient protocol was developed for shoot proliferation and plant regeneration ofPhlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub ofBangladesh, through in vitro culture using shoot tip and nodal explants. Best shootinduction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2%of nodal explants responded to produce maximum number (12.4 ± 0.66) of shootsper culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA +0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rootingculture tubes were kept in normal room temperature for 7 days beforetransplanting in pots where plantlets were reared for three weeks. The survivalrate of regenerated plantlets was 85%.

Ando H.,Okayama University of Science | Ando H.,Kobe Skin Research Institute | Niki Y.,Kobe Skin Research Institute | Ito M.,Niigata University | And 4 more authors.
Journal of Investigative Dermatology | Year: 2012

Recent studies have described the role of shedding vesicles as physiological conveyers of intracellular components between neighboring cells. Here we report that melanosomes are one example of shedding vesicle cargo, but are processed by a previously unreported mechanism. Pigment globules were observed to be connected to the filopodia of melanocyte dendrites, which have previously been shown to be conduits for melanosomes. Pigment globules containing multiple melanosomes were released from various areas of the dendrites of normal human melanocytes derived from darkly pigmented skin. The globules were then captured by the microvilli of normal human keratinocytes, also derived from darkly pigmented skin, which incorporated them in a protease-activated receptor-2 (PAR-2)-dependent manner. After the pigment globules were ingested by the keratinocytes, the membrane that surrounded each melanosome cluster was gradually degraded, and the individual melanosomes then spread into the cytosol and were distributed primarily in the perinuclear area of each keratinocyte. These results suggest a melanosome transfer pathway wherein melanosomes are transferred from melanocytes to keratinocytes via the shedding vesicle system. This packaging system generates pigment globules containing multiple melanosomes in a unique manner. © 2012 The Society for Investigative Dermatology.

PubMed | Biological Research Division
Type: Journal Article | Journal: Cytotechnology | Year: 2012

NS0 has been used as a fusion partner for the production of hybridomas and has more recently been engineered to produce recombinant protein. A protein-free culture medium, designated W38 medium, has previously been developed which supported high density growth of rat myeloma and hybridoma cell lines. NS0 cells failed to grow in W38 medium and in a number of protein-free culture media which support the growth of other myeloma cell lines. NS0 cells are derived from the NS-1 cell line, which is known to require exogencus cholesterol. It was found that NS0 cells grew in W38 medium supplemented with phosphatidylcholine, cholesterol, and albumin and that NS0 were auxotrophic for cholesterol. Protein-free growth of NS0 cells was achieved by using -cyclodextrin to replace albumin as a lipid carrier. The maximal cell density reached in this protein-free medium was in excess of 1.510(6) cell ml(-1). The lipid supplements in the medium precipitated after a few days storage at +4C. In order to overcome this problem a protocol was developed which allowed NS0 cells to be adapted to cholesterol-independent growth in W38 medium. NS0.CF (cholesterol-independent NS0 cells) were cultured continuously in W38 medium for several months. In shake flask culture a cell density of 2.410(6) cells ml(-1) was achieved in W38 medium compared with 1.4110(6) cells ml(-1) in RPMI 1640 medium containing 10% foetal bovine serum. NS0.CF cells readily grew in a 1 litre stirred bioreactor using W38 medium supplemented with Pluronic F68 reaching a density of 3.2410(6) cells ml(-1). NS0.CF were cloned protein-free by limiting dilution in W38 medium, giving colonies in wells that were seeded at an average density of 0.32 cells per 200 l. This study has demonstrated for the first time the growth of a cholesterol-requiring mouse myeloma cell line in a completely defined protein-free medium and its subsequent adaptation to cholesterol-independence.

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