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Dhaka, Bangladesh

Sinha P.,Biological Research Division | Jahan M.A.A.,Biological Research Division
Plant Tissue Culture and Biotechnology | Year: 2012

For high frequency regeneration of Rhyncnostylis retusa (Lin.) Blume apical nodal segments were used. Half strength MS + 2% sucrose + 1.5 mg/l BA + 0.5 mg/l NAA + 2 g/l peptone + 10% (v/v) coconut water (CW) + 0.5 g/l activated charcoal (AC) was the best nutrient medium, on which 89% cultures induced 8 microshoots per culture. Subculture of microshoots for further 8 weeks on the same nutrient medium enhanced the number of microshoots up to 95. For further proliferation of microshoots, their development into shoots as well as formation of secondary microshoots from the base of the old ones, the best medium was half strength of MS + 2% sucrose + 2 g/l peptone + 10% (v/v) CW + 0.5 g/l AC + 150 mg/l L-glutamine. Plantlets with roots were obtained in half strength of MS + 2% sucrose + 2 g/l peptone + 10% (v/v) CW + 0.5 g/l AC + 5.0 g/l banana powder, on which cent per cent shoots rooted within eight weeks. The pH of all the categories of cultures were maintained at 5.6 before adding 2.2 g/l gelrite and autoclaving, and the cultures were incubated at 2000 - 3000 lux for 16/8 hrs light/dark at 24 ± 2oC. Regeneration of plantlets continued due to repeated subculture of microshoots and regenerants were acclimatized and established in the nursery. Source


Shahariar M.S.,Biological Research Division | Tahsin S.,Biological Research Division | Gani M.N.,Bangladesh Jute Research Institute BJRI | Huq S.M.I.,University of Dhaka
Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences | Year: 2012

The growth and biomass productivity of kenaf (Hibiscus cannabinus L.) grown with different sources of organic waste viz. sewage sludge, poultry litter, cow dung and rice straw application were observed in a field experiment. Organic wastes were applied at the rate of 5 t/ha and were compared with recommended dose of fertilizers and control. The plants were harvested at 120 days after sowing (at the flowering stage). Different sources of organic wastes had a significant effect (P < 0.05) on the growth indices and biomass productivity of kenaf. Increased plant growth and biomass productivity were in the order of sewage sludge > cow dung>poultry litter > rice straw treatments. Among the four sources of organic wastes, sewage sludge treated plot produced the highest mean biomass of 23.33 t/ha (dry weight basis) which was 14.64% higher than the mean biomass production from control plot. Source


Hassan S.K.M.,Biological Research Division | Khatun R.,Biological Research Division
Bangladesh Journal of Botany | Year: 2010

Shoot tips and nodal explants from in vitro growing seedlings of Ficus glomerata Roxb. (Moraceae). showed best shoot induction (88%) on MS medium supplemented with 0.5 mg/1 BAP, where maximum number of shoots were produced per culture. In vitro raised shoots rooted well on half strength of MS medium with 2.0 mg/1 IB A + 0.1 mg/1 NAA. The survival rate of regenerated plantlets was 82%. Source


Ando H.,Okayama University of Science | Ando H.,Kobe Skin Research Institute | Niki Y.,Kobe Skin Research Institute | Ito M.,Niigata University | And 4 more authors.
Journal of Investigative Dermatology | Year: 2012

Recent studies have described the role of shedding vesicles as physiological conveyers of intracellular components between neighboring cells. Here we report that melanosomes are one example of shedding vesicle cargo, but are processed by a previously unreported mechanism. Pigment globules were observed to be connected to the filopodia of melanocyte dendrites, which have previously been shown to be conduits for melanosomes. Pigment globules containing multiple melanosomes were released from various areas of the dendrites of normal human melanocytes derived from darkly pigmented skin. The globules were then captured by the microvilli of normal human keratinocytes, also derived from darkly pigmented skin, which incorporated them in a protease-activated receptor-2 (PAR-2)-dependent manner. After the pigment globules were ingested by the keratinocytes, the membrane that surrounded each melanosome cluster was gradually degraded, and the individual melanosomes then spread into the cytosol and were distributed primarily in the perinuclear area of each keratinocyte. These results suggest a melanosome transfer pathway wherein melanosomes are transferred from melanocytes to keratinocytes via the shedding vesicle system. This packaging system generates pigment globules containing multiple melanosomes in a unique manner. © 2012 The Society for Investigative Dermatology. Source


Matsui M.S.,Biological Research Division | Petris M.J.,University of Missouri | Karaman-Jurukovska N.,Biological Research Division | Muizzuddin N.,Biological Research Division | And 2 more authors.
Journal of Investigative Dermatology | Year: 2015

Omeprazole is a proton pump inhibitor used in the treatment of peptic ulcer disease and gastrosophageal reflux disease and acts by irreversibly blocking ATP4A, a P-type H+/K+ ATPase in gastric parietal cells. We found that omeprazole and its closely related congeners inhibited melanogenesis at micromolar concentrations in B16 mouse melanoma cells, normal human epidermal melanocytes, and in a reconstructed human skin model. Omeprazole topically applied to the skin of UV-irradiated human subjects significantly reduced pigment levels after 3 weeks compared with untreated controls. Omeprazole had no significant inhibitory effect on the activities of purified human tyrosinase or on the mRNA levels of tyrosinase, dopachrome tautomerase, Pmel17, or MITF mRNA levels. Although melanocytes do not express ATP4A, they do express ATP7A, a copper transporting P-type ATPase in the trans-Golgi network that is required for copper acquisition by tyrosinase. ATP7A relocalization from the trans-Golgi network to the plasma membrane in response to elevated copper concentrations in melanocytes was inhibited by omeprazole. Omeprazole treatment increased the proportion of EndoH sensitive tyrosinase, indicating that tyrosinase maturation was impaired. In addition, omeprazole reduced tyrosinase protein abundance in the presence of cycloheximide, suggestive of increased degradation. Our findings are consistent with the hypothesis that omeprazole reduces melanogenesis by inhibiting ATP7A and by enhancing degradation of tyrosinase. © 2015 The Society for Investigative Dermatology. Source

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