Dai L.,Affiliated Hospital |
Tian X.,Affiliated Hospital |
Tan N.,Biological Experiment Center |
Wen H.,Biological Experiment Center |
Huang L.,Biological Experiment Center
Chinese-German Journal of Clinical Oncology | Year: 2011
Objective: Colon cancer metastasis is the key in fertility rate of colon cancer. Many recent results about metastasis research indicated that EMMPRIN played an important role in cancer metastasis. So, we designed this experiment to investigate whether EMMPRIN can enhance the metastatic ability of murine colon adenocarcinoma cell, CT26. Methods: EMMPRIN was over expressed in CT26 cells through transfecting pCMV-HA2-EMMPRIN into the CT26 cells. Invasion assay, wound migration assay and adhesion assay were utilized to analyze the metastasis of CT26 cells in vitro after EMMPRIN over expression. Results: After EMMPRIN over expression, invasion assay showed that invasive cells were 103.33 + 8.49 in EMMPRIN group and 48.67 + 5.3 in control group (P < 0.001). Migration assay showed that migrating cells were 40.67 + 2.49 in EMMPRIN group and 18.33 + 2.05 in control group (P < 0.001). CCK-8 absorbance value in adhesion assay were 3.33 + 0.17 in EMMPRIN group and 2.10 + 0.22 in control group (P < 0.001). Conclusion: Over expression of EMMPRIN could enhance the CT26 cell capacity of invasion and migration, and inhibit CT26 cell capacity of adhesion remarkably. The results suggest that EMMPRIN may be involved in cancer metastasis and play an important role in promotion of cancer metastasis. © 2011 Springer-Verlag Berlin Heidelberg.