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Bothell, WA, United States

Mathew A.,BioLife Solutions Inc.
Journal of Visualized Experiments | Year: 2010

Cryopreservation efficacy - which includes post-thaw recovery, viability, and functionality is of importance to both research and clinical applications. The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis. Cells and tissues can be prepared and preserved in ultra low temperature environments (-80°C to -196°C) using CryoStor cryopreservation solutions. Since these solutions are formulated to address the molecular biological aspects of cells during the cryopreservation process, they reduce the level of cryopreservation-induced delayed-onset cell death, thereby improving post-thaw cell viability and function. In addition, they need to include serum, proteins, or high levels of cytotoxic agents is eliminated with this protocol. In this video article, we will demonstrate the procedures for freezing, storage, and thawing of cells, as well as a viability assessment of the cells post-thaw. © 2010 Journal of Visualized Experiments.

BioLife Solutions Inc. | Date: 2010-04-29

Provided are apparatuses for cryopreserving cells which include a vessel comprising a biocompatible substrate, wherein the vessel further comprises an interior and an exterior, and a mechanical ice nucleating device disposed in or on the vessel interior for initiating ice crystal formation. Also provided are kits comprising one or more apparatuses for cryopreserving cells and a biopreservation medium. Further provided are compositions comprising a vessel for holding cells, a mechanical ice nucleating device, a biopreservation medium, and cells disposed in the vessel. The apparatuses, kits, and compositions of the invention can optionally include an insulating material which is disposed on all or a portion of the vessel.

The present invention is directed to systems and proteogenomic methods for predicting the success of the transplant of a cell, tissue, or organ by providing a means to determine the quality of the cell, tissue, or organ to be transplanted. In one embodiment, the present invention uses samples from the preservation solution to obtain phenomic fingerprints correlated with transplant pre-operative and post-operative data as a pre-operative tissue diagnostic and procedural success predictive indicator.

BioLife Solutions Inc. | Date: 2013-03-15

Chemical preparations for scientific purposes, namely, biopreservation media formulations including hypothermic storage and cryopreservation solutions for use with cells and tissue in low temperature environments in academic and clinical research. Chemical solutions for clinical use in storing and treating cells and biological tissue in low temperature environments, namely, biopreservation media formulations in the nature of hypothermic storage and cryopreservation solutions.

Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase II | Award Amount: 522.62K | Year: 2001


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