Cheongju, South Korea
Cheongju, South Korea

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Park S.C.,Hannam University | Park S.C.,Bioland Co. | Oh S.H.,Hannam University | Lee J.H.,Hannam University
Tissue Engineering and Regenerative Medicine | Year: 2011

An asymmetrically porous nerve guide conduit (NGC) with selective permeability and hydrophilicity was prepared by rolling an asymmetrically porous poly(dioxanone-b-caprolactone) (PDOCL)/Pluronic F127 membrane fabricated by a novel immersion precipitation method. The asymmetrically porous PDOCL/Pluronic F127 membrane was fabricated by immersing the PDOCL/Pluronic F127 solution (in tetraglycol) in a mold into water. The PDOCL/Pluronic F127 mixture was precipitated in water by the diffusion of water into PDOCL/Pluronic F127 mixture solution. The prepared PDOCL/F127 NGC had an asymmetric column-shape pore structure. The inner surface of the tube had sub-micron pores (< 1 μm) that can prevent fibrous tissue infiltration but permeate nutrients/ oxygen, and the outer surface had micron pores (∼ 200 μm) that can allow vascular ingrowth for the effective supply of nutrients and oxygen into the NGC to promote nerve regeneration. The PDOCL/F127 NGC with 3 wt% Pluronic F127 appeared to be optimal for its mechanical properties and hydrophilicity. To stimulate nerve regeneration, NGF was immobilized onto the pore surfaces of the NGC via heparin binding. The NGF immobilized on the heparinbound NGC was released in a sustained manner for up to 35 days. The NGF-immobilized asymmetrically porous PDOCL/F127 (3 wt%) NGC may be a good candidate as a NGC for enhanced nerve regeneration.


Lee G.-C.,Korea Institute of Water and Environment | Jeon E.-S.,Bioland Ltd. | Le D.T.,Research Team for Vectorborne Diseases | Kim T.-S.,Inha University | And 3 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2011

Plasmodium falciparum and P. vivax malaria are endemic to many parts of the world and humans can be co-infected with both species. Because each Plasmodium species has different biological and clinical characteristics, accurate differentiation of the infecting species is essential for effective treatment. Therefore, we produced three monoclonal antibodies that recognize the lactate dehydrogenase of P. falciparum, P. vivax, or both to develop the first P. falciparum, P. vivax, and mixed-species infections malaria antigen detection kit. The detection limits of this kit were 150 and 250 parasites/μL for P. falciparum and P. vivax, respectively, and the kit was able to detect mixed-species infections. The sensitivity and specificity of this kit was assessed with 722 clinical specimens. Our results showed that its sensitivities for P. falciparum, P. vivax, and mixed-species infection were 96.5%, 95.3%, and 85.7%, respectively. In addition, its specificity was high (99.4%). Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.


Choi H.-R.,Seoul National University | Kang Y.-A.,Seoul National University | Na J.-I.,Seoul National University | Huh S.Y.,Seoul National University | And 3 more authors.
Journal of Cosmetic Dermatology | Year: 2012

Backgrounds: Hyaluronic acid (HA) is an abundant matrix component and is degraded into polymers of various sizes. These generated fragments appear to have properties that affect wound healing of the skin. In industry, small-sized HA is used as a moisturizing agent but can have biologic effects when it is absorbed through the skin with barrier disruption. Aims: In this study, the regenerative effects of these molecules were investigated using skin equivalent (SE) models. Methods: Normal human keratinocytes and fibroblasts were isolated, and the effects of oligosaccharides of HA were tested in cultured keratinocytes and in the SE model. Results: In the monolayer of cultured keratinocytes, oligosaccharides of HA did not affect the proliferation of keratinocytes. However, the epidermis became thicker when oligosaccharides of HA were added during the culture of SE models. The data also showed that oligosaccharides of HA promote the differentiation of the epidermis. Furthermore, the expression of p63, integrin-α6 and integrin-β1 was increased. Western blot analysis also showed increased expression of both integrins. Conclusions: These findings suggest that oligosaccharides of HA increase the differentiation of the epidermis. In addition, increased number of p63, a putative stem cell marker of the skin, showed that oligosaccharides of HA promote the survival of basal stem cells by modulating the expression of integrin-α6 and integrin-β1. Finally, it can be said that inflammation-induced small-sized oligosaccharides can have beneficial effects on epidermal regeneration and topically applied oligosaccharide of HA can have healing effects in skin problems. © 2012 Wiley Periodicals, Inc..


Lee Y.K.,Chungbuk National University | Choi I.S.,Chungbuk National University | Ban J.O.,Chungbuk National University | Lee H.J.,Chungbuk National University | And 7 more authors.
Journal of Nutritional Biochemistry | Year: 2011

Oxidative stress induced neuronal cell death by accumulation of β-amyloid (Aβ) is a critical pathological mechanism of Alzheimer's disease (AD). Intracerebroventrical infusion of Aβ1-42 (300 pmol/day per mouse) for 14 days induced neuronal cell death and memory impairment, but pre-treatment of 4-O-methylhonokiol (4-O-MH), a novel compound extracted from Magnolia officinalis for 3 weeks (0.2, 0.5 and 1.0 mg/kg) prior to the infusion of Aβ1-42 and during the infusion dose dependently improved Aβ1-42-induced memory impairment and prevented neuronal cell death. Additionally, 4-O-MH reduced Aβ1-42 infusion-induced oxidative damages of protein and lipid but reduced glutathione levels in the cortex and hippocampus. Aβ1-42 infusion-induced activation of astrocytes and p38 mitogenic activated protein (MAP) kinase was also prevented by 4-O-MH in mice brains. In further study using culture cortical neurons, p38 MAP kinase inhibitor abolished the inhibitory effect of 4-O-MH (10 μM) on the Aβ1-42 (5 μM)-induced reactive oxidative species generation and neuronal cell death. These results suggest that 4-O-MH might prevent the development and progression of AD through the reduction of oxidative stress and neuronal cell death via inactivation of p38 MAP kinase pathway. © 2011 Elsevier Inc.


Lee Y.J.,Chungbuk National University | Choi I.S.,Chungbuk National University | Park M.H.,Chungbuk National University | Lee Y.M.,Chungbuk National University | And 10 more authors.
Free Radical Biology and Medicine | Year: 2011

Presenilin 2 (PS2) mutation increases Aβ generation and neuronal cell death in the brains of Alzheimer disease (AD) patients. In a previous study, we showed that increased oxidative damage and activation of extracellular signal-regulated kinase (ERK) were associated with Aβ generation and neuronal cell death in neuronal cells expressing mutant PS2. In this study, we show that oral treatment with 4-O-methylhonokiol, a novel compound isolated from Magnolia officinalis, for 3 months (1.0 mg/kg) prevented PS2 mutation-induced memory impairment and neuronal cell death accompanied by a reduction in Aβ1-42 accumulation. We also found that 4-O-methylhonokiol inhibited PS2 mutation-induced activation of ERK and β-secretase, and oxidative protein and lipid damage, but recovered glutathione levels in the cortex and hippocampus of PS2 mutant mice. Additionally, 4-O-methylhonokiol prevented PS2 mutation-induced activation of astrocytes as well as production of TNF-α, IL-1β, reactive oxygen species (ROS), and nitric oxide (NO) in neurons. Generation of TNF-α, IL-1β, ROS, and NO and ERK activation in cultured astrocytes treated with lipopolysaccharide (1 μg/ml) were also prevented by 4-O-methylhonokiol in a dose-dependent manner. These results suggest that the improving effects of 4-O-methylhonokiol on memory function may be associated with a suppression of the activation of ERK and astrocytes as well as a reduction in oxidative damage. Thus, 4-O-methylhonokiol may be useful in the prevention and treatment of AD. © 2010 Elsevier Inc. All rights reserved.


Lee J.W.,Chungbuk National University | Lee Y.K.,Chungbuk National University | Lee B.J.,Chungbuk National University | Nam S.-Y.,Chungbuk National University | And 5 more authors.
Pharmacology Biochemistry and Behavior | Year: 2010

The components of the herb Magnolia officinalis have exhibited antioxidant and neuroprotective activities. In this study, we investigated effects of ethanol extract of M. officinalis and its major component 4-O-methylhonokiol on memory dysfunction and neuronal cell damages caused by Aβ. Oral pretreatment of ethanol extract of M. officinalis (2.5, 5 and 10mg/kg) and 4-O-methylhonokiol (1mg/kg) into drinking water for 5weeks suppressed the intraventricular treatment of Aβ1-42 (0.5μg/mouse, i.c.v.)-induced memory impairments. In addition, 4-O-methylhonokiol prevented the Aβ1-42-induced apoptotic cell death as well as β-secretase expression.4-O-methylhonokiol also inhibited H2O2 and Aβ1-42-induced neurotoxicity in cultured neurons as well as PC12 cells by prevention of the reactive oxygen species generation. 4-O-methylhonokiol also directly inhibited β-secretase activity and Aβ fibrilization in vitro. Thus, ethanol extract of M. officinalis may be useful for prevention of the development or progression of AD, and 4-O-methylhonokiol may be a major active component. © 2009 Elsevier Inc.


Patent
Bioland Ltd. | Date: 2010-04-15

The present invention relates to a method for preparing a contact lens-shaped amniotic dressing and a contact lens-shaped amniotic dressing prepared therefrom for treating ocular surface diseases, which does not require the use of sutures or an adhesion material. The inventive contact lens-shaped amniotic dressing is capable of solving the problems associated with suturing an amniotic membrane, e.g., highly delicate surgical techniques of suturing, long surgery time, stitch abscess, granuloma formation, tissue necrosis, and discomfort of patients; and the problems associated with the use of a support, e.g., the elimination of the support by eye blinking, breaking of the support, and discomfort.


Disclosed is a composition for treating or preventing amyloid-related diseases including 4-O-methylhonokiol as an active ingredient. More specifically, a pharmaceutical composition including 4-O-methylhonokiol, which is effective for treating or preventing amyloid-related diseases such as Alzheimers disease, cognitive disorder, defective memory, amyloidosis, etc. is disclosed. The inventors of the present disclosure have found out for the first time that 4-O-methylhonokiol inhibits the production of -amyloid. It has been confirmed to be useful in treating or preventing amyloid-related diseases. Through animal tests including water maze test and passive avoidance test on mice, 4-O-methylhonokiol has been confirmed to be effective for amyloid-related diseases such as Alzheimers disease, defective memory, cognitive disorder, and the like. It was further confirmed through acetylcholinesterase activity inhibition test using mouse brain cortex and hippocampus tissue that they are particularly effective in treating or preventing Alzheimers disease among the amyloid-related diseases.


Trademark
Bioland Corporation | Date: 2015-04-27

Casein dietary supplements; Dietary fiber to aid digestion; Dietary supplements; Enzyme dietary supplements; Food supplements; Lecithin for use as a dietary supplement; Medicated lozenges; Mineral food supplements; Mineral nutritional supplements; Nutritional supplements; Vitamins and vitamin preparations.


Trademark
Bioland Corporation | Date: 2016-01-04

Enzyme dietary supplements; Enzyme food supplements; Mineral supplements; Nutritional supplements; Protein dietary supplements; Protein supplements; Vitamins.

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