Biohealth Convergence Center

Daegu, South Korea

Biohealth Convergence Center

Daegu, South Korea

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Lee S.-W.,Kyungpook National University | Yoon S.-R.,BioHealth Convergence Center | Kim G.-R.,Kyungpook National University | Woo S.-M.,Keimyung University | And 4 more authors.
Food Science and Biotechnology | Year: 2012

The effects of different nuruk contents and fermentation methods (AV, vinegar fermented in an agitated culture; SV, vinegar fermented in a static culture) on organic acids and volatile compounds in brown rice vinegars were investigated. In the SV, the contents of acetic, oxalic, tartaric, and malic acids increased with hipher contents of nuruk. Acetic, tartaric, and malic acid contents were higher in the SV than those in the AV. Volatile compounds that can affect vinegar quality, including acetic acid, isoamyl acetate, phenethyl acetate, and phenethyl alcohol were present at high concentrations in the AV. With the increase in nuruk contents in the AV, acetic acid content decreased and isoamyl acetate and phenethyl acetate content increased. No significant differences in sensory scores were observed regarding the amount of nuruk and the type of fermentation. However, electronic-nose analysis showed its potential to effectively differentiate different samples. © 2012 The Korean Society of Food Science and Technology and Springer Netherlands.


Kim B.J.,Biohealth Convergence Center | Son W.R.,Biohealth Convergence Center | Choi M.O.,Biohealth Convergence Center | Jo S.K.,Biohealth Convergence Center | And 4 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2013

Atopic dermatitis (AD) is a common skin disease characterized by chronic and relapsing inflammatory dermatitis with immunological disturbances. In spite of the continuous increase in the incidence of AD, it is regrettable that till date there is no effective treatment to treat the same. Therefore, the present study was designed to examine the possible anti-atopic effects of Castanea crenata inner shell extracts fermented by Lactobacillus bifermentans (FCS) in 2,4-dinitrochlorobenzene (DNCB) induced AD in NC/Nga mice. Based on the results of HPLC analysis, we found that FCS contains anti-inflammatory factors such as gallic acid (10.18 mg/g) and ellagic acid (2.14 mg/g). The groups that we have used in this study included 0.1%, 1%, 5% fermented Castanea crenata inner shell extracts (FCS 0.1, FCS 1, FCS 5), 1,3-butylene glycol treated control (AD), and normal mice. After topical FCS treatment, we observed that the clinical severity score for AD was lower in both the FCS 1 and FCS 5 groups than the AD group. We also proved beyond doubt that there was improvement of melanin, erythema and skin moisture indices in the FCS 5 group. Spleen index and gene expression levels of pro-inflammatory cytokines such as IL-1β and TNF-α were significantly decreased in the FCS 5 group compared to the AD group (P<0.05). Further, we also found that the level of serum immunoglobulin E (IgE) in the FCS-treated group was decreased in a concentration-dependent manner. The results of our study suggest that FCS can be effectively used as a cosmeceutical ingredient for both the prevention and improvement of AD. © 2013 by The Korean Society of Food Science and Nutrition. All rights reserved.


Akram K.,Kyungpook National University | Ahn J.-J.,Kyungpook National University | Yoon S.-R.,BioHealth Convergence Center | Kim G.-R.,Kyungpook National University | Kwon J.-H.,Kyungpook National University
Postharvest Biology and Technology | Year: 2012

Quality attributes (appearance, color, texture, weight, and volatile compounds) of irradiated (0, 1, 2, and 3) king oyster (Pleurotus eryngii) mushrooms, packed in polystyrene trays and covered with polyvinylchloride (PVC) film, were evaluated over a storage period of 4 weeks at 5 ± 1 °C. Hunter L values (lightness) increased upon irradiation and remained high throughout the storage period in 1. kGy-irradiated samples, whereas a values showed a slight decrease, and b values exhibited an increasing trend following irradiation. Mushroom texture decreased in firmness with increasing dose, but 1. kGy-irradiated samples maintained an overall better texture than all other samples. A similar trend was observed for weight loss during storage. The control samples developed mold, whereas 3. kGy-irradiated mushrooms were soft at the 3rd week of storage. Scanning electron microscopy showed micro-structural changes in the irradiated samples, which were probably the cause for increased weight loss and poor texture at the high 3. kGy dose. A principal component analysis of E-nose data showed dose-dependent differences in volatile profiles of all samples. Irradiation at 1. kGy was most effective for the extension of postharvest storage with added advantages. © 2011 Elsevier B.V..


Jung H.K.,BioHealth Convergence Center | Kim K.-S.,Kyungpook National University | Jeong Y.S.,BioHealth Convergence Center
Journal of the Korean Society of Food Science and Nutrition | Year: 2014

In this study, the anti-inflammatory effects of Lespedeza cuneata extract on macrophages and wound-healing in wound-induced animal experiments were investigated. In an anti-inflammatory test, 0.1 mg/mL of Lespedeza cuneata extract did not affect growth of RAW 264.7 cells, and Lespedeza cuneata extract suppressed nitric oxide (NO) generation from inflammation-induced macrophages in a concentration-dependent manner. Wounds on the skin of rats were treated with vehicle containing Lespedeza cuneata extract (SSP), vehicle (SCO), and commercial ointment (CCO). The wound and scar sizes in the SSP group were significantly reduced in comparison to the SCO and CCO groups (P<0.05). The epidermis and dermis of the SSP group also recovered faster than the SCO group based on Masson's trichrome staining. The gene expression levels of vascular endothelial growth factor (VEGF) de- creased and transforming growth factor-beta 1 (TGF-β1) increased in wound tissue from the SSP group compared to that from the SCO group. These results show that Lespedeza cuneata extract accelerates wound-healing through anti-inflammatory activity and induction of collagen regeneration as well as reduces the scar area surrounding wounds. Accordingly, Lespedeza cuneata extract could be useful as a cosmeceutical in the cosmetic industry.


Kim B.J.,Biohealth Convergence Center | Jung H.K.,Biohealth Convergence Center | Jeong Y.S.,Biohealth Convergence Center | Yang S.J.,Catholic University of Daegu | Hong J.-H.,Catholic University of Daegu
Applied Biological Chemistry | Year: 2016

Constipation is a common cause of discomfort that affects the quality of life. The aim of the present study was to evaluate the ameliorative effects of microencapsulated food products using Bacillus subtilis CBD2 strain-fermented grain in mice with loperamide-induced constipation. Microcapsules prepared by spray-drying with 2 % pectin and 2 % alginic acid (SD-P2A2) showed higher adhesion and dissolution of amylase than those prepared by freeze-drying. Experimental mice were fed a diet containing SD-P2A2 for 7 days prior to loperamide treatment. The ameliorative effects of an experimental diet containing SD-P2A2 on constipation were compared with those observed in mice fed a control diet containing non-fermented grain. Following the induction of constipation in mice, a marked alteration was observed in fecal parameters (frequency of defecation, fecal weights, fecal water content, and fecal pellet numbers in the distal colon), gastrointestinal transit ratios, harmful enzyme activities, and gene expression differences related to intestinal muscle contraction. These symptoms were significantly ameliorated in mice fed the experimental diet with SD-P2A2 versus constipated control mice. Thus, the intake of microencapsulated food products using B. subtilis CBD2 strain-fermented grain exhibited probiotic effects; these products may be used as a functional material for reducing constipation. © 2016, The Korean Society for Applied Biological Chemistry.


Jeong Y.S.,Biohealth Convergence Center | Jung H.K.,Biohealth Convergence Center | Hong J.-H.,Catholic University of Daegu
Journal of the Korean Society of Food Science and Nutrition | Year: 2013

We investigated the protective effect of UVB inducing photodamage from mulberry extract (ME) and Lithospermum erythrorhizon extract (LE). The contents of total anthocyanin and shikonin as a color compound of ME and LE were 4.92 mg/g and 9.58 mg/g, respectively. The electron donating ability and superoxide radical scavenging activity of ME were 84.32% and 76.34%, respectively. The oxygen radical absorbance capacity of the ME (545.37 μmoles TE/g) was higher than LE (427.18 μmoles TE/g). MMP-1 production in the HS68 cells were exposed to UVB suppressed by treatment with 200 μg/mL of ME (68.6%) and LE (32.7%). ME and LE were applied to a skin aging mouse model, which was induced by the irradiation of UVB to the backs of hairless mice. The value of skin erythema index, wrinkle depth and thickness, epidermis thickness, and collagenous fiber damage in the experiment groups (MEL: ME 3%, MEM: ME 5%, MEH: ME 7%, LEL: LE 3%, LEM: LE 5%, LEH: LE 7%) were remarkably reduced than in the control group (only UVB exposure group), while water capacity increased. The level of total wrinkles depth in the skin was decreased to be 30% of the control group by MEH and LEM. These results suggest that ME and LE are useful cosmetic materials for skin protection against UVB-inducing.


Jeong Y.S.,Biohealth Convergence Center | Jung H.K.,Biohealth Convergence Center | Hong J.-H.,Catholic University of Daegu
Journal of the Korean Society for Applied Biological Chemistry | Year: 2013

This study presented a multiplex, single-tube, realtime polymerase chain reaction (RTi-PCR) approach for detecting Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella typhimurium, three of the more frequent foodborne pathogenic bacteria typically investigated in a variety of foods. New primer sequences were designed for detection of specific gene fragments in the 23s ribosomal RNA, transmembrane transcription regulator, and replication origin sequences of S. aureus, V. parahaemolyticus, and S. typhimurium. Simultaneous amplifications were performed under the optimized reaction conditions. Melting curve analysis using SYBR Green I RTi-PCR analysis produced characteristic Tm values for each target amplicon, demonstrating specific and efficient amplification of the three fragments. Addition of an internal amplification control did not affect detection sensitivity for the target pathogen. The analysis of frequent foodborne pathogenic bacteria in artificially inoculated food demonstrated analytical sensitivity for direct detection of each pathogen using the Chelex method rather than a commercial DNA extraction kit. The assay was sensitive to 103 colony-forming units (CFU)/reaction. With enrichment (2 or 4 h), each species could be detected at 101 CFU/g. These results provided that RTi-PCR is a rapid and costeffective procedure to detect foodborne pathogens. This assay could become a valuable tool for routine microbiological analysis in the food industry. © 2013 The Korean Society for Applied Biological Chemistry.


Hong J.-H.,Catholic University of Daegu | Jung H.K.,Biohealth Convergence Center
Journal of the Korean Society for Applied Biological Chemistry | Year: 2014

β-Glucan isolated from cell wall of fungi not only has low purity and yield, but also causes adverse effects. Consequently, extracellular β-glucan produced by microorganisms is the focus of this study. β-Glucan-rich exopolysaccharides (G-EPSs) with different molecular weights from Paenibacillus polymyxa JB115 were prepared by ultrafiltration and spray-drying. The weight-average molecular masses of the G-EPSs, P-SD-1 (spray-dried G-EPSs powder prepared below 100 kDa) and P-SD-2 (spray-dried G-EPSs powder prepared above 100 kDa), were 7.084×104 and 9.235×105 g/mol, respectively. β-Glucan content was 47.38% in P-SD-1 and 73.12% in P-SD-2. The hydroxyl radical- and superoxide radicalscavenging activities of P-SD-2 at 1 mg/mL (39.45 and 87.34%, respectively) were higher than those of P-SD-1 (30.32 and 53.06%, respectively). Maximal nitric oxide (22.24±1.34 μM) was generated in the presence of P-SD-2 (1 mg/mL) and the antitumor activity of P-SD-2 was higher than P-SD-1 in four tumor cell lines (HeLa, Sarcoma 180, A549, and Hep3B cells). Thus, antioxidant and antitumor activities could be enhanced by regulating the molecular weight of G-EPSs. We anticipate that the food and medicinal use of G-EPSs will follow further characterization of this class of exopolysaccharides. © 2014 The Korean Society for Applied Biological Chemistry.


Kim B.J.,Biohealth Convergence Center | Hong J.-H.,Catholic University of Daegu | Jeong Y.S.,Biohealth Convergence Center | Jung H.K.,Biohealth Convergence Center
Journal of the Korean Society for Applied Biological Chemistry | Year: 2014

Probiotics are live microbes that confer health benefits on the host when administered in adequate amounts. To evaluate the probiotic potential of Bacillus subtilis isolated from Korean fermented foods, we investigated the resistance to biological barriers and improvement of loperamide-induced constipation. The values of resistance to gastric acidity of B. subtilis CBD2 and KMKW4 strains were 55.34±2.12 and 64.58±2.95%, respectively, whereas the survival rate of B. subtilis KMKW4 (31.17±5.78%) in bile acids was superior to that of CBD2 (8.62±2.09%). These strains also demonstrated adhesiveness to intestinal epithelial HT-29 cells and an inhibitory activity against pathogenic microflora. Furthermore, B. subtilis CBD2 and KMKW4 strains improved gastrointestinal activity when tested in a loperamide-induced mouse model of constipation. Pre-treatment with CBD2 and KMKW4 strains before the onset of constipation improved fecal output and gastrointestinal transit in loperamide-treated mice. These strains also showed inhibitory effects on the activity of β-glucosidase and tryptophanase, harmful enzymes of intestinal microflora. Taken together, these finding show that B. subtilis CBD2 and KMKW4 have high adaptability to gastrointestinal environment, and the ability to inhibit pathogenic microflora and prevent constipation, suggesting their activity as potential probiotics. © 2014, The Korean Society for Applied Biological Chemistry.


Kim D.,Yeungnam University | Kim D.,Hokkaido University | Park J.-H.,BioHealth Convergence Center | Kweon D.-J.,BioHealth Convergence Center | Han G.D.,Yeungnam University
International Journal of Nanomedicine | Year: 2013

Background: The aim of this study was to enhance the bioavailability of conjugated linoleic acid (CLA), which has low water solubility, using nanoemulsion technology and to evaluate the effects of its improved bioavailability as an antiobesity agent. Methods: The antiobesity effect of nanoemulsified water-soluble conjugated linoleic acid (N-CLA) was evaluated using in vitro and in vivo studies. Differentiated 3T3-L1 adipocytes were treated with CLA and N-CLA to assess their lipolytic effect. Further, to confirm the antiobesity effect of N-CLA, male Sprague-Dawley rats were randomly separated into four groups, ie, a group fed a normal diet, a group fed a high-fat diet (obesity rat model), a CLA-treated group, and an N-CLA-treated group. Results: N-CLA showed a greater lipolytic effect on differentiated 3T3-L1 adipocytes compared with normal CLA. N-CLA enhanced the release of glycerol from triglycerides, which accumulated in differentiated 3T3-L1 adipocytes. Further, N-CLA enhanced leptin secretion to an extent similar to that of orlistat, an antiobesity agent. In an animal obesity model fed a high-fat diet, N-CLA attenuated accumulation of triglycerides, total cholesterol, and low-density lipoprotein cholesterol in serum, and also significantly decreased the volume of triglycerides and cholesterol in liver tissue. Conclusion: These results indicate that N-CLA has a greater antiobesity effect than CLA as a result of its improved bioavailability. © 2013 Kim et al, publisher and licensee Dove Medical Press Ltd.

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