Daejeon, South Korea
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Cho H.-J.,Seoul National University | Yoon I.-S.,Seoul National University | Yoon H.Y.,Korea Institute of Science and Technology | Koo H.,Korea Institute of Science and Technology | And 7 more authors.
Biomaterials | Year: 2012

Polyethylene glycol (PEG)-conjugated hyaluronic acid-ceramide (HACE) was synthesized for the preparation of doxorubicin (DOX)-loaded HACE-PEG-based nanoparticles, 160 nm in mean diameter with a negative surface charge. Greater uptake of DOX from these HACE-PEG-based nanoparticles was observed in the CD44 receptor highly expressed SCC7 cell line, compared to results from the CD44-negative cell line, NIH3T3. A strong fluorescent signal was detected in the tumor region upon intravenous injection of cyanine 5.5-labeled nanoparticles into the SCC7 tumor xenograft mice; the extended circulation time of the HACE-PEG-based nanoparticle was also observed. Pharmacokinetic study in rats showed a 73.0% reduction of the in vivo clearance of DOX compared to the control group. The antitumor efficacy of the DOX-loaded HACE-PEG-based nanoparticles was also verified in a tumor xenograft mouse model. DOX was efficiently delivered to the tumor site by active targeting via HA and CD44 receptor interaction and by passive targeting due to its small mean diameter (<200 nm). Moreover, PEGylation resulted in prolonged nanoparticle circulation and reduced DOX clearance rate in an in vivo model. These results therefore indicate that PEGylated HACE nanoparticles represent a promising anticancer drug delivery system for cancer diagnosis and therapy. © 2011 Elsevier Ltd.


Cho H.-J.,Seoul National University | Yoon H.Y.,Korea Institute of Science and Technology | Koo H.,Korea Institute of Science and Technology | Ko S.-H.,Biogenics Inc. | And 6 more authors.
Biomaterials | Year: 2011

Hyaluronic acid-ceramide (HA-CE)-based self-assembled nanoparticles were developed for intravenous docetaxel (DCT) delivery. In this study, physicochemical properties, cellular uptake efficiency, and in vivo targeting capability of the nanoparticles developed were investigated. DCT-loaded nanoparticles composed of HA-CE and Pluronic 85 (P85) with a mean diameter of 110-140. nm were prepared and their morphological shapes were assessed using transmission electron microscopy (TEM). DCT release from nanoparticle was enhanced with increasing P85 concentrations in our in vitro model. Blank nanoparticles exhibited low cytotoxicity in U87-MG, MCF-7 and MCF-7/ADR cell lines. From cellular uptake studies, the nanoparticles developed enhanced the intracellular DCT uptake in the CD44-overexpressing cell line (MCF-7). The nanoparticles were shown to be taken up by the HA-CD44 interaction according to DCT and coumarin 6 (C6) cellular uptake studies. The multidrug resistance (MDR)-overcoming effects of DCT-loaded HA-CE/P85-based nanoparticles were also observed in cytotoxicity tests in MCF-7/ADR cells. Following the intravenous injection of DCT-loaded cyanine 5.5 (Cy5.5)-conjugated nanoparticles in MCF-7/ADR tumor-bearing mice, its in vivo targeting for CD44-overexpressing tumors was identified by non-invasive near-infrared (NIR) fluorescence imaging. These results indicate that the HA-CE-based nanoparticles prepared may be a promising anti-cancer drug delivery system through passive and active tumor targeting. © 2011 Elsevier Ltd.


Jin Y.-J.,Seoul National University | Termsarasab U.,Seoul National University | Ko S.-H.,Biogenics Inc. | Shim J.-S.,Biogenics Inc. | And 6 more authors.
Pharmaceutical Research | Year: 2012

Purpose: Hyaluronic acid-ceramide (HACE)-based nanoparticles (NPs) were developed for the targeted delivery of doxorubicin (DOX), and their antitumor efficacy for melanoma was evaluated. Methods: DOX-loaded HACE-based self-assembled NPs were prepared and their physicochemical properties were characterized. The in vitro cytotoxicity of HACE was measured using an MTS-based assay. The cellular uptake efficiency of DOX into mouse melanoma B16F10 cells was assessed by confocal laser scanning microscopy and flow cytometry. Tumor growth and body weight were monitored after the intratumoral and intravenous injection of DOX-loaded NPs into a B16F10 tumor-bearing mouse model. Results: DOX-loaded NPs, with a mean diameter of ∼110 nm, a narrow size distribution, and high drug entrapment efficiency, were prepared. A sustained DOX release pattern was shown, and drug release was enhanced at pH 5.5 compared with pH 7.4. The cytotoxicity of HACE to B16F10 cells was negligible. It was assumed that DOX was taken up into the B16F10 cells through receptor-mediated endocytosis. A significant inhibitory effect was observed on tumor growth, without any serious changes in body weight, after the injection of DOX-loaded NPs into the B16F10 tumor-bearing mouse model. Conclusions: DOX-loaded HACE-based NPs were successfully developed and their antitumor efficacy against B16F10 tumors was demonstrated. © Springer Science+Business Media, LLC 2012.


Provided is a three-layered capsule using a hollow silica capsule containing a stabilizer, a hydrogel polymer consisting of a polymer blend, and an appropriately hydrophobic polymer. The principal components of the present invention include a single-layered capsule formed from water-insoluble substances, a stabilizer, and a hollow silica; a double-layered capsule having a hydrogel polymer blend surrounding the outside of the single-layered capsule; and a three-layered capsule having a hydrophobic polymer surrounding the outside of the double-layered capsule.


The present invention relates to a three-layered capsule using a hollow silica capsule containing a stabilizer, a hydrogel polymer consisting of a polymer blend, and an appropriately hydrophobic polymer. The principal components of the present invention include a single-layered capsule formed from water-insoluble substances, a stabilizer, and a hollow silica; a double-layered capsule having a hydrogel polymer blend surrounding the outside of the single-layered capsule; and a three-layered capsule having a hydrophobic polymer surrounding the outside of the double-layered capsule.


Disclosed herein are a color capsule composition, which contains a polymer and a plasticizer swelling the polymer so as to allow the capsule particles to easily break and in which the capsule particles have a porous structure that boosts the effects thereof, and a method for preparing the color capsule composition. The preparation method comprises: uniformly mixing a color pigment, a plasticizer and a polymer in a first solvent to produce a first mixture solution; spray-drying the first mixture solution to produce core particles in which the color pigment is covered by the polymer; uniformly mixing the obtained core particles, a functional pigment and a second solvent to produce a second mixture solution; and spray-drying the second mixture solution to produce in which a coating layer of the functional pigment is formed on the outer surface of the polymer.


The present invention relates to a capsule composition containing UV-blocking inorganic nanoparticles, and a preparation method thereof, in which the UV-blocking inorganic nanoparticles are embedded in hydrophilic polymer capsules using a water-soluble dispersing agent, so that when the composition is applied to the skin, the polymer forms a thin hydrogel film that keeps the inorganic nanoparticles contained in the capsules to prevent the inorganic nanoparticles from penetrating the skin. The method comprises: uniformly mixing UV-blocking inorganic nanoparticles with a water-soluble dispersing agent in water to prepare a dispersion of the inorganic nanoparticles coated with the dispersing agent; adding, to the dispersion, either a hydrophilic polymer capable of forming a hydrogel, or a solution of the hydrophilic polymer in distilled water, to prepare a mixture; and spray-drying the mixture, thereby producing capsule particles containing the dispersing agent-coated UV-blocking inorganic nanoparticles covered by a film formed of the hydrophilic polymer.


Trademark
Biogenics Inc. | Date: 2010-08-10

therapeutic mattresses and mattress pads.


Provided is a method of solubilizing a poorly soluble/insoluble active material through formation of an oligomer composite, in which a structure having a hydrophobic cavity structure is formed by using oligomers derived from two types of hydrophilic natural polymers and a poorly soluble/insoluble component is encapsulated in the cavity structure, and thus, self-aggregation of the poorly soluble/insoluble material is prevented and simultaneously, thermodynamic stability increases to effectively solubilize the poorly soluble/insoluble material. According to the constitution of the present invention, the method may include a first operation of preparing an oligomer composite having a cavity structure formed therein by mixing and dissolving oligomers derived from two types of hydrophilic natural polymers in water, and a second operation of adding a poorly soluble/insoluble material to the oligomer composite to encapsulate the poorly soluble/insoluble material in the hydrophobic cavity structure of the oligomer composite.


A capsule composition containing UV-blocking inorganic nanoparticles, and a preparation method thereof, in which the UV-blocking inorganic nanoparticles are embedded in hydrophilic polymer capsules using a water-soluble dispersing agent, so that when the composition is applied to the skin, the polymer forms a thin hydrogel film that keeps the inorganic nanoparticles contained in the capsules to prevent the inorganic nanoparticles from penetrating the skin. The method comprises: uniformly mixing UV-blocking inorganic nanoparticles with a water-soluble dispersing agent in water to prepare a dispersion of the inorganic nanoparticles coated with the dispersing agent; adding, to the dispersion, either a hydrophilic polymer capable of forming a hydrogel, or a solution of the hydrophilic polymer in distilled water, to prepare a mixture; and spray-drying the mixture, thereby producing capsule particles containing the dispersing agent-coated UV-blocking inorganic nanoparticles covered by a film formed of the hydrophilic polymer.

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