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Grant
Agency: European Commission | Branch: FP7 | Program: CP | Phase: ENERGY.2009.3.3.1 | Award Amount: 17.46M | Year: 2010

Economic and sustainable production of fuels, chemicals and materials from biomass requires capture of the maximum energy and monetary value from sustainable feedstock. SUPRA-BIO achieves this by focussing on innovative research and development of critical unit operations, by using process intensification to match economic production to the scale of available feedstock and by process integration that provides energy from process waste, optimises utilities to minimise environmental impact and maximises value from the product mix. A technology toolbox for conversion and separation operations is developed that adapts to various scenarios of product mix and feedstock. These are contextualized by full life cycle and economic analysis of potential biorefinery schemes. Based on lignocellulose, microbial/organic waste or microalgae feedstock, innovation and intensification are used to improve the economics and carbon efficiency of fractionation, separation, bio and thermochemical conversions to produce biofuels, intermediates and high value products. Strain selection, genetic manipulation, molecular design and nanocatalysis are used to improve productivity and selectivity; reactor design, intensification and utilities integration for economics. Fermentation to 2,3 butanediol is demonstrated. Mono and multiculture processes are researched for high value products and feedstock streams. Separation is developed for omega oils and specific lignochemicals. Nano and biocatalytic processes are developed for biofuels and bioactive molecules. Integration into potential biorefinery schemes is explored in laboratory pilots of integrated reactors, by piloting on sidestreams, by exchanging separated fractions between partners and by process evaluations. The project includes all the scientific, engineering and industrial skills required to produce the step changes required for biorefineries to impact significantly on realising the aims of the European Strategic Energy Technology Plan


Grant
Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: ENERGY-2007-3.3-02 | Award Amount: 4.97M | Year: 2008

The EU target to increase the use of renewable energy carriers in the transportation sector in the coming years has already started to boost the production of biodiesel from rapeseed and other vegetable oils. This is leading to an immense increase in the production of glycerol, which is an unavoidable by-product from the esterification process. Since the volume of the glycerol-byproduct has already started to exceed the current market need, the biodiesel producers are requesting new methods for sustainable glycerol management. The GLYFINERY project is targeted to development of a novel technology based on biological conversion of the glycerol by-product into known and new, advanced liquid biofuels, bioenergy and valuable biochemicals in an integrated biorefinery concept. Implementation of the glycerol bioprocessing line into the biodiesel plant will improve the economics of the biodiesel biorefinery by enhanced energy conversion efficiency of the traditional raw materials and by generating valuable, highly demanded bioproducts. The GLYFINERY project will achieve the aim of the highly efficient process scheme through a multidirectional effort using new biocatalysts tailored by genetic modification for specific conversion routes, highly efficient fermentation processes for maximum possible conversion of glycerol into the target products, highly efficient methods for product recovery and the overall process integration. The individual processes are proposed to be studied in the lab-scale, and the most promising process scheme will be tested in the pilot-scale as an integrated concept for demonstration of the suitability of the GLYFINERY concept implementation into the large-scale biorefineries. The environmental and economical assessment will be performed along with the technological assessment for obtaining of the overall, integrated assessment of the optimized multi-stage process.


Yao S.,Technical University of Denmark | Mikkelsen M.J.,BioGasol
Journal of Molecular Microbiology and Biotechnology | Year: 2010

Thermoanaerobacter mathranii contains four genes, adhA, adhB, bdhA and adhE, predicted to code for alcohol dehydrogenases involved in ethanol metabolism. These alcohol dehydrogenases were characterized as NADP(H)-dependent primary alcohol dehydrogenase (AdhA), secondary alcohol dehydrogenase (AdhB), butanol dehydrogenase (BdhA) and NAD(H)-dependent bifunctional aldehyde/alcohol dehydrogenase (AdhE), respectively. Here we observed that AdhE is an important enzyme responsible for ethanol production in T. mathranii based on the constructed adh knockout strains. An adhE knockout strain fails to produce ethanol as a fermentation product, while other adh knockout strains showed no significant difference from the wild type. Further analysis revealed that the ΔadhE strain was defective in aldehyde dehydrogenase activity, but still maintained alcohol dehydrogenase activity. This showed that AdhE is the major aldehyde dehydrogenase in the cell and functions predominantly in the acetyl-CoA reduction to acetaldehyde in the ethanol formation pathway. Finally, AdhE was conditionally expressed from a xylose-induced promoter in a recombinant strain (BG1E1) with a concomitant deletion of a lactate dehydrogenase. Overexpressions of AdhE in strain BG1E1 with xylose as a substrate facilitate the production of ethanol at an increased yield. Copyright © 2010 S. Karger AG, Basel.


Yao S.,Technical University of Denmark | Mikkelsen M.J.,BioGasol
Applied Microbiology and Biotechnology | Year: 2010

Thermoanaerobacter mathranii can produce ethanol from lignocellulosic biomass at high temperatures, but its biotechnological exploitation will require metabolic engineering to increase its ethanol yield. With a cofactor-dependent ethanol production pathway in T. mathranii, it may become crucial to regenerate cofactor to increase the ethanol yield. Feeding the cells with a more reduced carbon source, such as mannitol, was shown to increase ethanol yield beyond that obtained with glucose and xylose. The ldh gene coding for lactate dehydrogenase was previously deleted from T. mathranii to eliminate an NADH oxidation pathway. To further facilitate NADH regeneration used for ethanol formation, a heterologous gene gldA encoding an NAD+-dependent glycerol dehydrogenase was expressed in T. mathranii. One of the resulting recombinant strains, T. mathranii BG1G1 (Δldh, P xyl GldA), showed increased ethanol yield in the presence of glycerol using xylose as a substrate. With an inactivated lactate pathway and expressed glycerol dehydrogenase activity, the metabolism of the cells was shifted toward the production of ethanol over acetate, hence restoring the redox balance. It was also shown that strain BG1G1 acquired the capability to utilize glycerol as an extra carbon source in the presence of xylose, and utilization of the more reduced substrate glycerol resulted in a higher ethanol yield. © 2010 Springer-Verlag.


The invention relates to an apparatus for treating material by cutting, soaking and/or washing of the material, wherein the apparatus comprises a receptacle (10), a discharge element (20) with a vortex generator and pumping means (22) arranged to pump fluid and material from the receptacle (10) towards the vortex generator, wherein the vortex generator and pumping means (22) in combination are adapted to generate a vortex in the form of a conic helix in the fluid extending into the receptacle.


The invention relates to a method or methods of operating an apparatus for treating material by cutting, soaking and/or washing of the material, wherein the apparatus comprises a receptacle, a discharge element with a vortex generator and a pump arranged to pump fluid and material from the receptacle towards the vortex generator, wherein the vortex generator and the pump in combination are adapted to generate a vortex in the form of a conic helix in the fluid extending into the receptacle.


The invention relates to a method for isolating a microorganism containing a known genetic element. The method employs several rounds of 1) dilution of a mixed culture containing the selected microorganism in several replicates, 2) growing the replicates, 3) detecting the organism in at least one of the replicates and repeating steps 1) through 3) until the organism can be isolated by standard procedures.


Strict anaerobic thermophilic bacterium belonging to the group of Thermoanaerobacter italicus subsp. marato subsp. nov. and mutants and derivatives thereof. The bacterium is particularly suitable for the production of fermentation products such as ethanol, lactic acid, acetic acid and hydrogen from lignocellulosic biomass.


Patent
BioGasol | Date: 2010-01-12

The present invention relates to an apparatus, which can be part of a pre-treatment system in a plant for the production of fuels, e. g. bio-ethanol, derived from plant biomass, e. g. first generation crops, such as grain, sugarcane and corn or second generation crops such as lignocellulosic biomass. The invention relates to an apparatus for processing, such as fluffing and mixing, at least two media, such as a solid, e. g. biomass, and a fluid, e. g. steam, so as to rendering the first medium susceptible to efficient receiving of energy and/or mass which is provided by localized release of the second medium. Although the description of the present invention focuses on biomass, it is envisaged that the invention is generally applicable to control the mixing of at least two media by crossing their stream of while dispersing at least one of them.


Patent
BioGasol | Date: 2014-09-29

The present invention relates to an apparatus, which can be part of a pre-treatment system in a plant for the production of fuels, e. g. bio-ethanol, derived from plant biomass, e. g. first generation crops, such as grain, sugarcane and corn or second generation crops such as lignocellulosic biomass. The invention relates to an apparatus for processing, such as fluffing and mixing, at least two media, such as a solid, e. g. biomass, and a fluid, e. g. steam, so as to rendering the first medium susceptible to efficient receiving of energy and/or mass which is provided by localized release of the second medium. Although the description of the present invention focuses on biomass, it is envisaged that the invention is generally applicable to control the mixing of at least two media by crossing their stream of while dispersing at least one of them.

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