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Ghandinagar, India

Koradiya M.,Biogas Research Center | Duggirala S.,Biogas Research Center | Tipre D.,Gujarat University | Dave S.,Gujarat University
Bioresource Technology | Year: 2016

Based on one parameter at a time, saccharification of delignified sorghum biomass by 4% and 70% v/v sulfuric acid resulted in maximum 30.8 and 33.8 g% sugar production from biomass respectively. The Box Behnken Design was applied for further optimization of acid hydrolysis. As a result of the designed experiment 36.3 g% sugar production was achieved when 3% v/v H2SO4 treatment given for 60 min at 180 °C. The process was scaled-up to treat 2 kg of biomass. During the screening of yeast cultures, isolate C, MK-I and N were found to be potent ethanol producers from sorghum hydrolyzate. Culture MK-I was the best so used for scale up of ethanol production up to 25 L capacity, which gave a yield of 0.49 g ethanol/g sugar from hydrolyzate obtained from 2 kg of sorghum biomass. © 2015 Elsevier Ltd. Source

Patel M.V.,Biogas Research Center | Shilpkar P.,Biogas Research Center | Dungrechia A.,Biogas Research Center
Journal of Pure and Applied Microbiology | Year: 2015

In a laboratory study 18 different types of bacteria were isolated from soil showing protease production in 1% casein agar medium. After screening, one potent bacterial culture was identified as Bacillus thuringiensis and its protease production was further optimized for various fermentation conditions. Results show that the culture gives maximum alkaline protease production under optimum condition of pH-11.5 (40.7 U/mL), temperature-25°C (60.13 U/mL), substrate concentration-3% (33.54 U/mL) inoculum size - 1mL (v/v) with optimum activity 60.13 U/mL, incubation time-24 hr (60.13 U/mL), 0.30% beef extract as nitrogen source (61.23 U/mL), and 1% lactose as carbon source (37.77 U/mL). Source

Prajapati K.A.,Biogas Research Center | Shilpkar P.,Biogas Research Center | Dungrechiya A.,Biogas Research Center
Journal of Pure and Applied Microbiology | Year: 2015

Nine bacterial strains were isolated from the waste water collected from slaughter house of Sadra village, Gujarat, India. Among the isolates, one potent strain was selected on the basis of diameter of zone of clearance and enzyme activity. The strain was identified on the basis of microscopic, cultural and biochemical tests as Staphylococcus sciuri. Biochemical tests were performed by VITEK® 2 Systems Version: 05.04 at Supra Tech laboratory, Ahmedabad. Some fundamental parameters like effect of raw materials, substrate concentration, pH, temperature, incubation time and inoculum size, nitrogen and carbon sources for maximum protease production were also studied. Maximum yield of enzyme was obtained at pH of 9.0 with 3 mL inoculum size in the media containing 2% stem of Sorghum vulgare (jowar) as substrate, after 24 h of incubation in environmental shaker maintained at a temperature of 25°C along with maltose as carbon source and beef extract as nitrogen source. The study show that the Staphylococcus sciuri can efficiently produce alkaline protease using stem of Sorghum vulgare. Source

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