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Shah M.C.,Gujarat Vidyapeeth | Patel H.P.,Gujarat Vidyapeeth | Shilpkar P.G.,Biogas Research and Extension Center
Pollution Research | Year: 2015

Now a days many synthetic neutralization indicators are available and their number are continuously increasing. Industrial synthesis of indicators causes environmental pollution directly as well as indirectly. The present study deals with small effort to give alternate of synthetic neutralization indicators through principles of green chemistry. Lavender to pink coloured Argyreia nervosa flowers are available during July-December and March-April. The water extract 10% w/v shows different colours at different pH: Pink (0 < pH < 2.87); Light pink (2.87 ≤ pH < 3.09); Coolourless (3.09 ≤ pH < 6.00%) Green (6.9 ≤ pH) in pH metric titration of 0.1M HC1 → 0.1 M NaOH. Indicator properties for this extract may be due to change in structure of anthocyanine derivatives, present in flower with pH change. Water extract of Argyreia nervosa flowers is more suitable as an acid-base indicator in HCl → NaOH. It is not suitable for CH3COOH → NaOH and NH4OH → HCl titrations. Copyright © EM International. Source


Balapure K.H.,Biogas Research and Extension Center | Jain K.,Sardar Patel University | Chattaraj S.,Sardar Patel University | Bhatt N.S.,Biogas Research and Extension Center | Madamwar D.,Sardar Patel University
Journal of Hazardous Materials | Year: 2014

Mixed cultures BDN (BDN) proficient in decolourizing diazo dye-reactive blue 160 (RB160) consist of eight bacterial strains, was developed through culture enrichment method from soil samples contaminated with anthropogenic activities. The synthrophic interactions of BDN have led to complete decolourization and degradation of RB160 (100mg/L) within 4h along with co-metabolism of yeast extract (0.5%) in minimal medium. BDN microaerophilicaly decolourized even 1500mg/L of RB160 under high saline conditions (20g/L NaCl) at 37°C and pH 7.0. BDN exhibited broad substrate specificity and decolourized 27 structurally different dyes. The reductase enzymes symmetrically cleaved RB160 and oxidative enzymes further metabolised the degraded products and five different intermediates were identified using FTIR, 1HNMR and GC-MS. The phytotoxicity assay confirmed that intact RB160 was more toxic than dye degraded intermediates. The BDN was able to colonize and decolourized RB160 in soil model system in presence of indigenous miocroflora as well as in sterile soil without any amendment of additional nutrients, which signifies it useful and potential application in bioremediation. © 2014 Elsevier B.V. Source


Prajapati K.P.,Biogas Research and Extension Center | Shilpkar P.,Biogas Research and Extension Center | Shah M.C.,Biogas Research and Extension Center | Patel K.R.,Biogas Research and Extension Center
Journal of Pure and Applied Microbiology | Year: 2013

Madhuca indica flowers are well-known for their sugar and nutrients content due to which they are used widely for alcohol production. After alcohol production the leftover cake has no economic utilization. Present study first time reports the alcohol production potential of Madhuca indica flower's cake (6.2%) compared to Madhuca indica flowers (10.9%). Submerged fermentation was carried out in laboratory shake flask culture using Kluyveromyces marxianus which was isolated from curd, screened on the basis of alcohol production and identified by 16SrRNA technique. Fermentation was carried out using Madhuca indica flowers or its cake as substrate in fermentation medium for 36 hours at 30°C temperature in shake flask culture at 100 rpm. The fermented broths were analyzed for alcohol, total sugars, total acidity, volatile acid, and tannin content. CO2 content was estimated from collecting solution of 3N NaOH. Results show that after fermentation 10.9 & 6.2% alcohol, 117.2 & 109.15 μg/mL total sugars, 6.8 & 14.6 meq/L total acids, 4.1 & 1.1 g/L total volatile acids, 2.9 and 2.6g carbon dioxide, and 5.95 & 8.0 (μg) tannin were produced in Madhuca indica flowers and Madhuca indica flower's cake fermentation, respectively. Source

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