Balapure K.H.,Biogas Research and Extension Center |
Jain K.,Sardar Patel University |
Chattaraj S.,Sardar Patel University |
Bhatt N.S.,Biogas Research and Extension Center |
Madamwar D.,Sardar Patel University
Journal of Hazardous Materials | Year: 2014
Mixed cultures BDN (BDN) proficient in decolourizing diazo dye-reactive blue 160 (RB160) consist of eight bacterial strains, was developed through culture enrichment method from soil samples contaminated with anthropogenic activities. The synthrophic interactions of BDN have led to complete decolourization and degradation of RB160 (100mg/L) within 4h along with co-metabolism of yeast extract (0.5%) in minimal medium. BDN microaerophilicaly decolourized even 1500mg/L of RB160 under high saline conditions (20g/L NaCl) at 37°C and pH 7.0. BDN exhibited broad substrate specificity and decolourized 27 structurally different dyes. The reductase enzymes symmetrically cleaved RB160 and oxidative enzymes further metabolised the degraded products and five different intermediates were identified using FTIR, 1HNMR and GC-MS. The phytotoxicity assay confirmed that intact RB160 was more toxic than dye degraded intermediates. The BDN was able to colonize and decolourized RB160 in soil model system in presence of indigenous miocroflora as well as in sterile soil without any amendment of additional nutrients, which signifies it useful and potential application in bioremediation. © 2014 Elsevier B.V.
Shah M.C.,Gujarat Vidyapeeth |
Patel H.P.,Gujarat Vidyapeeth |
Shilpkar P.G.,Biogas Research and Extension Center
Pollution Research | Year: 2015
Now a days many synthetic neutralization indicators are available and their number are continuously increasing. Industrial synthesis of indicators causes environmental pollution directly as well as indirectly. The present study deals with small effort to give alternate of synthetic neutralization indicators through principles of green chemistry. Lavender to pink coloured Argyreia nervosa flowers are available during July-December and March-April. The water extract 10% w/v shows different colours at different pH: Pink (0 < pH < 2.87); Light pink (2.87 ≤ pH < 3.09); Coolourless (3.09 ≤ pH < 6.00%) Green (6.9 ≤ pH) in pH metric titration of 0.1M HC1 → 0.1 M NaOH. Indicator properties for this extract may be due to change in structure of anthocyanine derivatives, present in flower with pH change. Water extract of Argyreia nervosa flowers is more suitable as an acid-base indicator in HCl → NaOH. It is not suitable for CH3COOH → NaOH and NH4OH → HCl titrations. Copyright © EM International.
Prajapati K.P.,Biogas Research and Extension Center |
Shilpkar P.,Biogas Research and Extension Center |
Shah M.C.,Biogas Research and Extension Center |
Patel K.R.,Biogas Research and Extension Center
Journal of Pure and Applied Microbiology | Year: 2013
Madhuca indica flowers are well-known for their sugar and nutrients content due to which they are used widely for alcohol production. After alcohol production the leftover cake has no economic utilization. Present study first time reports the alcohol production potential of Madhuca indica flower's cake (6.2%) compared to Madhuca indica flowers (10.9%). Submerged fermentation was carried out in laboratory shake flask culture using Kluyveromyces marxianus which was isolated from curd, screened on the basis of alcohol production and identified by 16SrRNA technique. Fermentation was carried out using Madhuca indica flowers or its cake as substrate in fermentation medium for 36 hours at 30°C temperature in shake flask culture at 100 rpm. The fermented broths were analyzed for alcohol, total sugars, total acidity, volatile acid, and tannin content. CO2 content was estimated from collecting solution of 3N NaOH. Results show that after fermentation 10.9 & 6.2% alcohol, 117.2 & 109.15 μg/mL total sugars, 6.8 & 14.6 meq/L total acids, 4.1 & 1.1 g/L total volatile acids, 2.9 and 2.6g carbon dioxide, and 5.95 & 8.0 (μg) tannin were produced in Madhuca indica flowers and Madhuca indica flower's cake fermentation, respectively.
Acharya K.P.,Biogas Research and Extension Center |
Shilpkar P.,Biogas Research and Extension Center |
Shah M.C.,Biogas Research and Extension Center
Journal of Pure and Applied Microbiology | Year: 2016
Production of esterase enzyme which is the key enzyme for organophosphorus pesticide degradation from Bacillus subtilis KPA-1 was confirmed in present study. Esterase production from Bacillus subtilis KPA-1 was observed when inoculated in mineral salt medium and localization of esterase production was also determined and the esterase enzyme was found to be membrane bound enzyme. Various process parameters were optimized by conventional method for maximum esterase production by B. subtilis KPA-1. Results indicate that pH 8, incubation temperature of 40°C, incubation time of 72 hr, 48 hr old inoculum with 3% inoculum size, glucose as carbon source and ammonium sulfate as nitrogen source and 800ppm monocrotophos concentration were found to be optimum values for highest esterase production.