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Chengdu, China

Abbas G.,Zhejiang University | Abbas G.,University of Gujrat | Wang L.,Biogas Institute | Wang L.,Laboratory of Development and Application of Rural Renewable Energy | And 3 more authors.
Ecological Engineering | Year: 2015

The pilot-scale internal-loop-airlift bio-particle (ILAB) reactor was a novel configuration adopted to study the performance of simultaneous partial nitrification (PN) and anaerobic ammonia oxidation (ANAMMOX). The ILAB reactor was operated at different nitrogen loading rates and hydraulic retention times to investigate the nitrogen removal kinetics. The reactor showed good tolerance to substrate concentration shock while it was significantly affected by hydraulic shock. Five kinetic models including first-order model, Grau second-order model, Stover-Kincannon model, Monod model and Contois model were applied to steady-state experimental data to evaluate the substrate removal in the reactor. The Grau second-order model and Stover-Kincannon model were found to be more appropriate compared with other models to describe and predict the performance of the reactor. Both the models were evaluated by judging the linearity between experimental data and predicted values which confirmed their validity for the process. © 2014 Published by Elsevier B.V.


Qiu T.,Beijing Academy of Agriculture and Forestry Sciences | Sun X.,Beijing Academy of Agriculture and Forestry Sciences | Wang X.,Beijing Academy of Agriculture and Forestry Sciences | Han M.,Beijing Academy of Agriculture and Forestry Sciences | And 4 more authors.
Advanced Materials Research | Year: 2011

Biogas fermentation is always limited or affected at low temperature conditions, one of key factors may be physiological adaption of methanogenic communities to low temperature. Howerer, biogas process could be stimulated and enhanced with addition of enriched consortia. Here, six psychroactive methanogenic consortiums were enriched as additives, and consortium E-1 was found to be most effective at 150C. The total biogas production addition with E-1 improved 39.3 % and 17.0% from pig manure and cow manure, respectively, when decreasing from 210C to 150C, which is a potential microbial addition for biogas fermentation at low temperature. © (2011) Trans Tech Publications.


Feng Y.,Key Laboratory of Energy Microbiology and Application | Feng Y.,Biogas Institute | Cheng L.,Key Laboratory of Energy Microbiology and Application | Cheng L.,Biogas Institute | And 7 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2T, was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous togalike structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7-0.9 mm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 °C, 7.0 and 15 g l1, respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2T fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, L-alanine, H 2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2T (=ACCC 00496T=DSM 22460T). © 2010 IUMS.


Yang C.,Sichuan Normal University | Yang C.,Biogas Institute | Wang W.-G.,Biogas Institute | Wang W.-G.,Key Laboratory of Development and Application of Rural Renewable Energy | And 5 more authors.
Huanjing Kexue/Environmental Science | Year: 2015

A Chlorella strain tolerant to high-strength anaerobic digestion effluent was isolated from the anaerobic digestion effluent with a long-term exposure to air. The strain was identified as a Chlorella by morphological and molecular biological methods, and named Chlorella sp. BWY-1. The anaerobic digestion effluent used in this study was from a biogas plant with the raw materials of swine wastewater after solid-liquid separation. The Chlorella regularis (FACHB-729) was used as the control strain. The comparative study showed that Chlorella sp. BWY-1had relatively higher growth rate, biomass accumulation capacity and pollutants removal rate in BG11 and different concentrations of anaerobic digestion effluent. Chlorella sp. BWY-1 had the highest growth rate and biomass productivity (324.40 mg·L-1) in BG11, but its lipid productivity and lipid content increased with the increase of anaerobic digestion effluent concentration. In undiluted anaerobic digestion effluent, the lipid productivity and lipid content of Chlorella sp. BWY-1 were up to 44.43% and 108.70 mg·L-1, respectively. Those results showed that the isolated algal strain had some potential applications in livestock wastewater treatment and bioenergy production, it could be combined with a solid-liquid separation, anaerobic fermentation and other techniques for processing livestock wastewater and producing biodiesel. ©, 2015, Science Press. All right reserved.

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