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Chaudhary N.,Chonnam National University | Bhardwaj J.,Chonnam National University | Hwang J.-H.,Chonnam National University | Seo H.-J.,Chonnam National University | And 5 more authors.
Biotechnology and Bioprocess Engineering | Year: 2015

Camellia sinensis L. O. Kuntze has traditionally been used in Asian countries as a stimulant, a diuretic, and to prevent obesity. Studies in green tea are well-documented in literature, though the potential of fruit from the plant has never been studied in details. In the present study, the anti-adipogenic effect of aqueous extract of green tea seed cake extract (GTE) were analysed in-vitro and the anti-obesity effects were determined in-vivo in murine model fed high-fat and high-carbohydrate diets. We found that GTE inhibited the lipid accumulation in 3T3-L1 adipocyte and significantly lowered body weight, body fat-pad weights, blood lipid, glucose, insulin, and leptin levels in mice fed high-fat and high-carbohydrate diets. GTE suppressed the expression of important adipogenic genes (PPARγ, C/EBPα, SREBP, LPL, aP2 and leptin) and lipid metabolism genes (FAS). Furthermore, GTE induced the levels of adiponectin in serum and adipose tissue, as well as AMP-activated protein kinase (AMPK) in liver tissue. These results suggested that GTE ameliorates its anti-obesity effect through multiple cells signaling pathways in diets induced obesity in mice. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.


Wee J.-H.,Food Research Center | Sung H.M.,Food Research Center | Jung K.O.,Food Research Center | Kim S.J.,Food Research Center | And 3 more authors.
Korean Journal of Food Science and Technology | Year: 2015

The effect of the hot water extract of defatted green tea seed cake (GTSE) on lipid metabolism and the underlying mechanisms of lipolysis in mature 3T3-L1 adipocytes were investigated. In this study, we found that the naringenin content of GTSE was 5.5 mg/g; however, catechins were not detected. The intracellular lipid droplets were stained with Oil Red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 52%, and intracellular triglyceride (TG) level was reduced by 33% after treatment with GTSE at a concentration of 40 μg/mL. To determine the mechanism of reduction in TG content, we determined the level of fatty acid synthase (FAS), phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), and acetyl-coenzyme A carboxylase (ACC) in the cell model. Incubation of the 3T3-L1 adipocytes with GTSE stimulated AMPK and ACC phosphorylation in a dosedependent manner, and decreased the expression of FAS. © The Korean Society of Food Science and Technology.


Jung H.J.,Food Research Center | Sung H.M.,Food Research Center | Kim K.M.,Food Research Center | Shin Y.-R.,BioFoodstory Co. | Wee J.-H.,Food Research Center
Journal of the Korean Society of Food Science and Nutrition | Year: 2015

The present investigation evaluated the antioxidant activities of water extracts from dandelion (Taraxacum officinale) aerial parts, roots, and mixed extracts. Mixed extract of T. officinale was a mixture of aerial parts and roots at 9:1 and 8:2 weight ratios. Extracts from aerial parts (DAE), roots (DRE), and mixture of aerial parts and roots (DME) were measured for cell viability and catalase activity in HepG2 cells, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and lipid peroxidation inhibitory activity. Cell viabilities of HepG2 cells treated with DAE, DRE, DME 8:2, and DME 9:1 against H2O2-induced oxidative damage were 63.4%, 54.6%, 76.7% and 83.4% at a concentration of 400 μg/mL, respectively. Catalase activity was highest in DME 9:1 (12.2 mU/min/mg protein) compared with DAE (9.0 mU/min/mg protein) and DRE (9.7 mU/min/mg protein). DPPH radical scavenging activity of DME showed a significantly lower EC50 value than DAE (EC50 value of DME 9:1=163.3 μg/mL, DME 8:2=172.4 μg/mL, and DAE=173.7 μg/mL). Lipid peroxidation inhibitory activity of DME showed a significantly lower EC50 value than DAE [EC50 values of DME (9:1)=454.4 μg/mL, DME (8:2)=426.6 μg/mL, and DAE=654.7 μg/mL]. The results indicate that a small amount of T. officinale roots increased antioxidant activity of aerial parts. Especially, a 9:1 mixture was more valuable than 8:2 mixture for industry. © 2015, Korean Society of Food Science and Nutrition. All rights reserved.


Wee J.-H.,Korea Food Research Institute | Jung H.J.,Korea Food Research Institute | Jung K.O.,Korea Food Research Institute | Sung H.M.,Korea Food Research Institute | And 6 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2015

In this study, the effect of Goheung pomegranate extract on postmenopausal syndrome was evaluated both in vitro and in vivo in ovariectomized Sprague-Dawly (SD) rats. Sixty female SD rats were divided into six groups: sham, sham operation and distilled water; OVX, ovariectomized and distilled water; PE1, ovariectomized and pomegranate concentrate (0.75 mL/twice/d); PE2, ovariectomized and pomegranate concentrate (1.5 mL/twice/d); PE3, ovariectomized and pomegranate concentrate (2.2 mL/twice/d); and CE, ovariectomized and commercial pomegranate concentrate (2.2 mL/twice/d). Percent bone volume (bone volume/tissue volume) and trabecular thickness (Tb.Th) improved in a dose-independent manner in PE1, 2, and 3. Especially, bone mineral density was significantly improved in PE3 (P<0.05) compared to OVX. Pomegranate extract reduced body weight and visceral fat mass. High density lipoprotein cholesterol (HDL-C) level slightly increased in a dose-independent manner in the experimental group. In addition, HDL-C/total cholesterol level of PE3 significantly increased (P<0.05) compared with OVX. These results show that pomegranate concentrate improved blood lipid levels and bone metabolism in ovariectomized rats. Therefore, Goheung pomegranate concentrates are expected to improve cardiovascular and bone-related diseases in menopausal women. © 2015, Korean Society of Food Science and Nutrition. All rights reserved.


Shin S.J.,BioFood Story Inc | Bae U.J.,Chonbuk National University | Ahn M.,Chonbuk National University | Ka S.O.,Chonbuk National University | And 6 more authors.
International Journal of Pharmacology | Year: 2013

Purple sweet potato is a widely consumed food around the world has been reported to possess antioxidant, antimutagenic and memory-enhancing effects. However, antiobesity effect of PSP is not clear. The objective of this study was to determine the effects of Purple Sweet Potato Extracts (PSPE) on serum and fecal lipid profiles, body weight gain, body fat percentage and hepatic lipogenesis. Mice were administered a standard chow diet, a 45% high-fat diet, or a high-fat diet with various doses of PSPE. Mice that were fed a high-fat diet containing PSPE were found to have lower increases in body and adipose tissue weights and lessened occurrences of hepatic steatosis than mice that were fed a high-fat diet without PSPE. The decreased adiposity induced by PSPE accounted for lower serum levels of leptin and a higher adiponectin/leptin ratio. PSPE administration also resulted in a significant decrease in serum and hepatic triglyceride and cholesterol levels and a significant increase in fecal triglyceride and cholesterol levels when compared to the high-fat group. To identify the mechanism by which PSPE induced its antiobesity effect, the expression of lipogenesis-related genes that were induced in high fat-fed mice was investigated. PSPE suppressed the expression of Sterol Regulatory Element-Binding Protein (SREBP)-l, Acyl-CoA Synthase (ACS), Glycerol-3-Phosphate Acyltransferase (GPAT), HMG-CoA Reductase (HMGR) and Fatty Acid Synthase (FAS) in liver tissue in mice provided the high-fat diet. These findings suggest that the antiobesity effect of PSPE in high fat-fed mice occurs through its modulation of lipogenesis in the liver and inhibition of dietary lipid absorption. © 2013 Asian Network for Scientific Information.

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