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Saint Beauzire, France

Badel S.,University Blaise Pascal | Bernardi T.,BioFilm Control | Michaud P.,University Blaise Pascal
Biotechnology Advances | Year: 2011

Lactobacilli have the ability to produce different kinds of exopolysaccharides (EPS) exhibiting a wide diversity of structures. EPS are classified, according to their composition into homopolysaccharides and heteropolysaccharides. One of their most described applications is their utilization as texturing agents naturally synthesized in the fermented food products. Nowadays, in regard to demand of modern consumers focusing towards safe and healthy food without additives, new perspectives of development appear for these biopolymers. The GRAS (Generally Recognized As Safe) and probiotic status of some lactobacilli give to them more preference for consumable EPS production. The main drawbacks limiting their industrial expansion are their low yields of production and the validation of their healthy allegations. Moreover, the texturing role of these exopolysaccharides, notably in dairy products, is actually a controversial issue. This review focuses on the novel ways of EPS production employing Lactobacillus spp. and their potential as nutraceuticals. © 2010 Elsevier Inc.


Badel S.,University Blaise Pascal | Laroche C.,University Blaise Pascal | Gardarin C.,University Blaise Pascal | Petit E.,Laboratoire des Polysaccharides Microbiens et vegetaux | And 2 more authors.
Enzyme and Microbial Technology | Year: 2011

The activity of polysaccharide cleavage enzymes has usually been evaluated by qualitative plate screening methods and quantitative colorimetric or chromatographic assays. The recent development of protein engineering has shown the limits of these techniques when applied to high throughput screening. Here we propose a microplate method to measure the activity of polysaccharide cleavage enzymes through small variations in viscosity. Polysaccharide solutions are co-incubated with magnetic particles in enzyme buffers. The cleavage action of polymer-degrading enzymes increases the mobility of the particles in a magnetic field, even at low levels of enzyme activities. This reproducible, sensitive technique was used to evaluate enzymatic specificity towards substrates. BioFilm indices (BFI) determined by associated software were used to follow enzyme kinetics and measure the usual variables. © 2010 Elsevier Inc.


Patent
Biofilm Control | Date: 2011-06-30

The present invention relates to a method for detecting molecular interactions in a solution. In particular, the present invention relates to a method for detecting interactions between two substances that are likely to interact with one another. The present invention can be used in particular in the field of scientific research and in the field of medical analysis.


Patent
Biofilm Control | Date: 2011-12-11

The present invention relates to a method for measuring the effect of a force on a film. In particular, the present invention relates to a method for measuring the effect of a mechanical, hydrodynamic or physical force, for example, on the integrity of a film, for example a film made of microorganisms, foodstuffs and/or chemical substances. The present invention is especially applicable to the fields of biology, chemistry, biotechnology and food processing.


Trademark
Biofilm Control | Date: 2012-09-21

Diagnostic preparations other than for medical or veterinary use. diagnostic reagents for medical use. apparatus and instruments for scientific research in laboratories, namely, air analysis apparatus, chemistry apparatus and instruments for testing the presence or the efficacy of antimicrobial agents, DNA chips, chromatography apparatus for laboratory use, computer programs used for testing the presence or the efficacy of antimicrobial agents; data processing apparatus, detectors of the presence or efficacy of antimicrobial agents; diagnostic apparatus, not for medical purposes, for testing the presence or the efficacy of antimicrobial agents; diagnostic apparatus for testing the presence or the efficacy of antimicrobial agents in food; incubators for bacteria culture, interfaces for computers, instruments and machines for testing the presence or the efficacy of antimicrobial agents, measures of the presence or the efficacy of antimicrobial agents, measuring apparatus of the presence or the efficacy of antimicrobial agents, measuring instruments of the presence or the efficacy of antimicrobial agents, observation instruments meant for testing the presence or the efficacy of antimicrobial agents; apparatus and instruments for physics meant for testing the presence or the efficacy of antimicrobial agents; probes meant for scientific purposes for testing the presence or the efficacy of antimicrobial agents; computer programs downloadable software for testing the presence or the efficacy of antimicrobial agents; scanners data processing equipment; apparatus, not for medical purposes, for testing the presence or the efficacy of antimicrobial agents. blood testing apparatus, catheters meant for testing the presence or the efficacy of antimicrobial agents; diagnostic apparatus for medical purposes meant for testing the presence or the efficacy of antimicrobial agents; medical apparatus and instruments meant for testing the presence or the efficacy of antimicrobial agents; urological imaging systems for detecting the presence or the efficacy of antimicrobial agents; veterinary apparatus and instruments meant for testing the presence or the efficacy of antimicrobial agents.

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