Biodynamic Plant Institute Co

Sapporo, Japan

Biodynamic Plant Institute Co

Sapporo, Japan

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Takahashi A.,Hokkaido University | Okazaki Y.,Fuji Women's University | Nakamoto A.,Fuji Women's University | Watanabe S.,Fuji Women's University | And 7 more authors.
Journal of Oleo Science | Year: 2014

Haskap (Lonicera caerulea L.) fruit contains some bioactive phenolic phytochemicals, mainly cyanidin-3-glucoside (cy3-glc) and chlorogenic acid. The purpose of this study was to investigate the effects of anthocyanin-rich phenolic phytochemical (containing 13.2% anthocyanin) purified from a Haskap fruit (named Haskap phytochemical) on postprandial serum triglyceride and blood glucose levels. The Haskap phytochemical (containing cy 3-glc at 300 mg/kg of body weight) was administered orally to rats fasted for 24 h and 30 min later, a corn oil emulsion was administered to these rats. After the administration, serum triglyceride concentration was measured. An increase in serum triglyceride concentration and the AUC significantly lowered in the Haskap phytochemical-administered group than in the saline-administered group. To evaluate the effect of serum glucose levels, the Haskap phytochemical was orally administered to rats fasted for 24 h and sucrose solution (2 g/kg of body weight) was administered to these rats after 30 min. After the administration, blood glucose level was measured. The Haskap phytochemical significantly reduced the increase in blood glucose levels and AUC in the Haskap phytochemical-administered group than in the saline-administered group. Furthermore, to investigate the long-term effects of Haskap phytochemical intake, high-fat diet (HF diet) with 1.5% or 3.0% Haskap phytochemical was administered to rats for four weeks. The investigation of chronological changes in the serum components of the rats fed HF diets in addition to the administration of Haskap phytochemical showed that the increase in serum triglyceride concentrations, total cholesterol concentrations and blood glucose were significantly suppressed compared to the HF diet-fed control (HF-control). These results suggest that the decrease in postprandial blood lipids and blood glucose by short or long-term Haskap phytochemical ingestion is due to anthocyanin and other polyphenols contained in the Haskap phytochemical. © 2014 by Japan Oil Chemists' Society.


Miyazawa T.,Tohoku University | Nakagawa K.,Tohoku University | Takekoshi H.,Sun Chlorella Co. | Higuchi O.,Biodynamic Plant Institute Co. | And 3 more authors.
Journal of Oleo Science | Year: 2013

Accumulation of phospholipid hydroperoxide (PLOOH) in erythrocyte membranes is an abnormality found in patients with senile dementia, including those with Alzheimer's disease. In our previous studies, dietary xanthophylls (polar carotenoids such as lutein) were hypothesized to inhibit lipid peroxidation. In the present study, we conducted a randomized, double-blind, placebo-controlled human trial to assess the impact for a total of 2 months Chlorella supplementation (8 g Chlorella/day/person; equivalent to 22.9 mg lutein/day/person) on PLOOH and carotenoid concentrations in erythrocytes as well as plasma of 12 normal senior subjects. After 1 or 2 months of treatment, erythrocytes and plasma lutein concentrations increased in the Chlorella group but not in the placebo group. In the Chlorella-supplemented group, erythrocyte PLOOH concentrations after a total of 2 months of treatment were lower than the concentrations before supplementation. These results suggest that Chlorella ingestion improved erythrocyte antioxidant status and lowered PLOOH concentrations. These reductions might contribute to maintaining the normal function of erythrocytes and prevent the development of senile dementia. © 2013 by Japan Oil Chemists' Society.


Miyazawa T.,Tohoku University | Nakagawa K.,Tohoku University | Kimura F.,Tohoku University | Nakashima Y.,Chlorella Industry Co. | And 2 more authors.
Journal of Oleo Science | Year: 2013

Chlorella contains a high amount of carotenoids, especially lutein, and has received attention as a possible dietary source for improving carotenoid levels in human blood. In the present study, we performed a 2-month single arm human study, and investigated the efficacy of Chlorella supplementation (9 g Chlorella/day; equivalent to 32 mg lutein/day) on lutein and other carotenoid concentrations in plasma as well as erythrocytes of 12 healthy subjects. Following Chlorella supplementation, lutein was the predominant carotenoid in erythrocytes, showing a 4-fold increase (from 14 to 54 pmol/mL packed cells). After the one month without Chlorella ingestion, erythrocyte lutein then decreased to a basal level (17 pmol/ mL packed cells). Erythrocyte carotenoid (lutein, zeaxanthin, α-carotene, and β-carotene) levels were proportional to plasma carotenoid levels. The results suggest the transfer of Chlorella carotenoids, especially lutein, from plasma lipoprotein particles to the erythrocyte membrane. Chlorella intake would be effective for improving and maintaining lutein concentrations in human erythrocytes. © 2013 by Japan Oil Chemists' Society.


Hori M.,Tohoku University | Komatsubara S.,Tohoku University | Nakagawa K.,Tohoku University | Higuchi O.,Biodynamic Plant Institute Co. | And 2 more authors.
Applied Entomology and Zoology | Year: 2014

Silkworms (Bombyx mori L.) accumulate 1-deoxynojirimycin (DNJ), a mulberry iminosugar, by feeding on mulberry leaves. DNJ is a potent α-glucosidase inhibitor that helps prevent diabetes. However, iminosugars are toxic to many insects. In this study, we analyzed the concentrations of three major mulberry iminosugars—DNJ, 2-O-α-d-galactopyranosyl-DNJ (GAL-DNJ), and fagomine—in the larvae of mulberry-feeding insect species (two mulberry specialists and six non-specialists), to clarify the differences in accumulation, metabolism, and excretion of iminosugars between specialists and non-specialists. DNJ and fagomine concentrations in the two Bombyx larvae were much higher than those in the other larvae. GAL-DNJ concentrations were low in all species. DNJ and fagomine concentrations in the excrements of Agrotis segetum (Denis and Schiffermüller) and Sarcopolia illoba (Butler) larvae were lower than in those of the other larvae. Further, iminosugar concentrations in the hemolymph of B. mori, B. mandarina Moore, and S. illoba larvae were analyzed. DNJ and fagomine concentrations in the hemolymph of the two Bombyx larvae were much higher than in that of S. illoba. DNJ concentrations in the whole bodies of the two Bombyx larvae decreased as they developed. Similarly, DNJ, and fagomine concentrations in the hemolymph of B. mori larvae decreased with growth. © 2014, The Japanese Society of Applied Entomology and Zoology.

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