Biodiversity and Integrated Gene Management Program BIGM

Cairo, Egypt

Biodiversity and Integrated Gene Management Program BIGM

Cairo, Egypt

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Mohamed O.E.,Agricultural Research Corporation | Hamwieh A.,Biodiversity and Integrated Gene Management Program BIGM | Ahmed S.,Biodiversity and Integrated Gene Management Program BIGM | Ahmed S.,Rabat Institute | Ahmed N.E.,Agricultural Research Corporation
Journal of Phytopathology | Year: 2015

Fusarium wilt caused by Fusarium oxysporum f.sp. ciceris (Foc) is the most important soilborne disease of chickpea in the Sudan and many other countries. A total of 76 Foc isolates from six different chickpea-growing states in the Sudan have been collected in this study to investigate the genetic diversity of Sudanese Foc isolates. Additional 14 Foc isolates from Syria and Lebanon were included in this study. All isolates were characterized using four random amplified polymorphic DNA (RAPD), three simple sequence repeats (SSR), five sequence-characterized amplified region (SCAR) primers and three specific Foc genome primers. Based on the similarity coefficient, the results indicated two major clusters included seven subclusters. The isolates from the Sudan were grouped as identified as races 0, 2 and unknown races. The isolates from Syria and Lebanon were grouped together as they identified as races 1B/C and 6, respectively. This study identified a new race Foc (race 0) in the Sudan. The results of this study will be useful for breeders to design effective resistance breeding program in chickpea in the Sudan. © 2015 Blackwell Verlag GmbH.

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