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Nablus, Palestinian Territory

Fuellgrabe M.W.,AlPlanta Institute for Plant Research | Boonrod K.,AlPlanta Institute for Plant Research | Jamous R.,AlPlanta Institute for Plant Research | Jamous R.,Biodiversity and Environmental Research Center | And 6 more authors.
Protein Expression and Purification | Year: 2011

HC-Pro is a helper component-proteinase which acts as a multifunctional protein in the potyviral life cycle. Apart from its proteolytic activity, HC-Pro has the capacity to bind duplex small RNAs (sRNAs). To investigate HC-Pro-mediated sRNA binding in vitro, high amounts of purified protein are required. For this purpose, the Zucchini yellow mosaic virus (ZYMV) HC-Pro was expressed as a fusion with hexa-histidine (6xHis) or maltose-binding protein (MBP) in Escherichia coli. The expressed fusion proteins were purified by affinity chromatography. 6xHis:HC-Pro and MBP:HC-Pro were partially soluble. Electrophoretic mobility-shift assays demonstrated that only MBP:HC-Pro exhibits the sRNA binding activity. The recombinant HC-Pro bound 21 bp siRNAs as well as 19 bp and 24 bp siRNAs. A point mutation in the highly conserved FRNK box produced the HC-Pro FINK protein, previously shown to be associated with reduced viral symptoms and weak sRNA binding. In this study, sRNA binding of the MBP:HA-HC-Pro FINK was not detectable. The high yield of purified HC-Pro offers the possibility to study the biochemistry of the protein in detail. © 2010 Elsevier Inc. All rights reserved.

Amessis-Ouchemoukh N.,University of Abderrahmane Mira de Bejaia | Abu-Reidah I.M.,University of Granada | Abu-Reidah I.M.,Functional Food Research and Development Center | Abu-Reidah I.M.,Biodiversity and Environmental Research Center | And 5 more authors.
Industrial Crops and Products | Year: 2014

The leaves of horehound (Marrubium vulgare), were screened for their phytochemical composition by the use of ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) together with the determination of antioxidant capacity of the methanol and acetone extracts. Total phenolic, flavonoid, and tannin contents were also determined by colorimetry. The antioxidant potential was assessed by DPPH• (1,1-diphenyl-2-picrilhydrazyl) radical scavenging activity, the scavenging activity against H2O2, total antioxidant capacity, and iron-reducing power. The UHPLC-MS/MS method of the methanolic extract revealed the presence of 34 compounds of different families, of which 17 compounds have been tentatively characterized for the first time. Flavonoids and phenylethanoid derivatives were the major compounds. Methanol extract contained more flavonoids and tannins, and the percentage of DPPH radical scavenging activity ranged from 51.90 to 97.15%. The ability of the methanol extract to scavenge H2O2 was considerable (59.73%). Total antioxidant capacity exhibited by methanol leaf extract was higher than that of acetone and in the ferric reducing power the absorbance augmented with increasing extract concentrations. The best overall antioxidant capacity was shown by the methanol leaf extract, indicating its use as a promising source of natural antioxidants for food preservation and prevention of oxidative-stress-related diseases. © 2014 Elsevier B.V.

Abu-Reidah I.M.,University of Granada | Abu-Reidah I.M.,Functional Food Research and Development Center | Abu-Reidah I.M.,Biodiversity and Environmental Research Center | del Mar Contreras M.,University of Granada | And 7 more authors.
Electrophoresis | Year: 2014

Vicia faba (Fabaceae) is a popular food in many countries and a good source of nutrients. However, little is known about its phytochemical composition, specially referring to phenolic compounds. In the present work, the dietary metabolites from a hydro-methanolic extract of V. faba seeds were thoroughly characterized by a nontargeted analytical approach based on reversed-phase ultra-HPLC (UHPLC) coupled to QTOF-MS. A total of 155 primary and secondary metabolites of various structural types were characterized: carbohydrates, amino acids, organic acids, alkaloids, terpenoids, jasmonates, and, mainly, polyphenols. Among the latter group, 73 compounds were characterized for the first time in this legume. In addition, 24 new structures, belonging to jasmonates and glycosylated N-containing compounds, were also proposed. Thus, this methodology could be implemented in foodomics as a characterization strategy to complement the knowledge of the phytochemical composition of vegetables. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Azaizeh H.,Haifa University | Azaizeh H.,Galilee College | Linden K.G.,University of Colorado at Boulder | Barstow C.,University of Colorado at Boulder | And 4 more authors.
Water Science and Technology | Year: 2013

Water shortage is an ongoing cardinal issue in the Middle East region. Wastewater reuse offers some remediation, but to-date many rural communities in the Palestinian Authority (PA) and in Jordan are not connected to centralized wastewater treatment plants (WWTPs), many of them are disposing of their wastewater using infiltration septic tanks. This highlights the need for a small, local, low cost WWTP that can directly benefit local communities, producing effluents suitable for unrestricted irrigation. Constructed wetlands (CWs) could offer a solution as they are relatively easy and cheap to construct and maintain, and effective in removal of many pollutants. Nevertheless, pathogen removal in CWs is often not adequate, calling for additional disinfection. Here we describe the use of low-cost, consumer level, UV based disinfection systems coupled to CWs for wastewater treatment in three CWs: in Israel, Jordan and in the PA. Once mature, our adapted CWs reduced chemical oxygen demand (COD) load, and, given proper use of the UV systems, inactivated indicator bacteria (faecal and E. coli) to levels suitable for irrigation, even when UV transmission (UVT) levels were low (∼40%). Our results demonstrate the promise in this combined treatment technique for cheap and simple wastewater treatment suitable for the Middle East region. © IWA Publishing 2013.

Amessis-Ouchemoukh N.,University of Abderrahmane Mira de Bejaia | Abu-Reidah I.M.,University of Granada | Abu-Reidah I.M.,Functional Food Research and Development Center | Abu-Reidah I.M.,Biodiversity and Environmental Research Center | And 9 more authors.
Phytochemical Analysis | Year: 2014

Introduction Globularia alypum L., belonging to the Globulariaceae family, is a perennial wild shrub found throughout the Mediterranean area, Europe, and Africa. This plant is widely used to treat many diseases, but no previous work on the phytochemical composition of the Algerian G. alypum species has yet been reported. Objective To investigate the phytoconstituents of the methanolic extract of G. alypum using an LC-ESI-QTOF-MS method. Methods Ground air-dried leaves of G. alypum were macerated with methanol at room temperature for 24 h. The supernatant was filtered and concentrated to dryness under reduced pressure in a rotary evaporator, and extracts were recovered with methanol and filtered. Afterwards, the G. alypum extract was injected into the LC-ESI-QTOF-MS system. Results The combined LC-MS/MS led to the tentative characterisation of 63 phytochemicals. In this work, a large number of compounds have been characterised in the leaf-extract analysis of this plant. Among others, 24 iridoids and secoiridoids were found, of which nine compounds have not previously been recorded in G. alypum. Also, nine unusual phenylethanoid glycosides were characterised for the first time in this species. Conclusion The method used has proved to be a valued tool for the characterisation of a wide range of compounds from G. alypum leaves. This work constitutes a detailed investigation of the chemical composition of G. alypum leaves, which are widely used in different traditional systems of medicine. Copyright © 2014 John Wiley & Sons, Ltd.

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