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Novickij V.,Vilnius Gediminas Technical University | Lastauskiene E.,Vilnius University | Svediene J.,Laboratory of Biodeterioration Research | Grainys A.,Vilnius Gediminas Technical University | And 6 more authors.
Journal of Membrane Biology | Year: 2017

Recently, a novel contactless treatment method based on high-power pulsed electromagnetic fields (PEMF) was proposed, which results in cell membrane permeabilization effects similar to electroporation. In this work, a new PEMF generator based on multi-stage Marx circuit topology, which is capable of delivering 3.3 T, 0.19 kV/cm sub-microsecond pulses was used to permeabilize pathogenic yeast Candida albicans separately and in combination with conventional square wave electroporation (8–17 kV/cm, 100 μs). Bursts of 10, 25, and 50 PEMF pulses were used. The yeast permeabilization rate was evaluated using flow cytometric analysis and propidium iodide (PI) assay. A statistically significant (P < 0.05) combinatorial effect of electroporation and PEMF treatment was detected. Also the PEMF treatment (3.3 T, 50 pulses) resulted in up to 21% loss of yeast viability, and a dose-dependent additive effect with pulsed electric field was observed. As expected, increase of the dB/dt and subsequently the induced electric field amplitude resulted in a detectable effect solely by PEMF, which was not achievable before for yeasts in vitro. © 2017 Springer Science+Business Media New York


Novickij V.,Vilnius Gediminas Technical University | Grainys A.,Vilnius Gediminas Technical University | Svediene J.,Laboratory of Biodeterioration Research | Paskevicius A.,Laboratory of Biodeterioration Research | And 2 more authors.
Biotechnology Progress | Year: 2016

The dermatophytes infect the skin by adherence to the epidermis followed by germination, growth, and penetration of the fungal hyphae within the cells. The aim of this study was to investigate the efficacy of the pulsed electric fields (PEF) of controlled inactivation of Trichophyton rubrum (ATCC 28188). In this work, we have used bursts of the square wave PEF pulses of different intensity (10–30 kV/cm) to induce the irreversible inactivation in vitro. The electric field pulses of 50 µs and 100 µs have been generated in bursts of 5, 10, and 20 pulses with repetition frequency of 1 Hz. The dynamics of the inactivation using different treatment parameters were studied and the inactivation map for the T. rubrum has been defined. Further, the combined effect of PEF with the antifungal agents itraconazole, terbinafine, and naftifine HCl was investigated. It has been demonstrated that the combined effect results in the full inactivation of T. rubrum colony. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1056–1060, 2016. © 2016 American Institute of Chemical Engineers


PubMed | Vilnius Gediminas Technical University and Laboratory of Biodeterioration Research
Type: Journal Article | Journal: Biotechnology progress | Year: 2016

The dermatophytes infect the skin by adherence to the epidermis followed by germination, growth, and penetration of the fungal hyphae within the cells. The aim of this study was to investigate the efficacy of the pulsed electric fields (PEF) of controlled inactivation of Trichophyton rubrum (ATCC 28188). In this work, we have used bursts of the square wave PEF pulses of different intensity (10-30 kV/cm) to induce the irreversible inactivation in vitro. The electric field pulses of 50 s and 100 s have been generated in bursts of 5, 10, and 20 pulses with repetition frequency of 1 Hz. The dynamics of the inactivation using different treatment parameters were studied and the inactivation map for the T. rubrum has been defined. Further, the combined effect of PEF with the antifungal agents itraconazole, terbinafine, and naftifine HCl was investigated. It has been demonstrated that the combined effect results in the full inactivation of T. rubrum colony. 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1056-1060, 2016.


Zalneravicius R.,Lithuanian Academy of Sciences | Paskevicius A.,Laboratory of Biodeterioration Research | Kurtinaitiene M.,Lithuanian Academy of Sciences | Jagminas A.,Lithuanian Academy of Sciences
Journal of Nanoparticle Research | Year: 2016

Abstract: The growing resistance of bacteria to conventional antibiotics elicited considerable interest to non-typical drugs. In this study, antimicrobial investigations were performed on low-size dispersion cobalt ferrite nanoparticles (Nps) fabricated by co-precipitation approach in several average sizes, in particular, 15.0, 5.0, and 1.65 nm. A variety of experimental tests demonstrated that the size of these Nps is determinant for antimicrobial efficiency against S. cerevisiae and several Candida species, in particular, C. parapsilosis, C. krusei, and C. albicans. The small and ultra-small fractions of CoFe2O4 Nps possess especially strong antimicrobial activity against all tested microorganisms. The possible reasons are discussed. Nps were characterized by means of transmission and high-resolution transmission electron microscopy, X-ray diffraction, energy dispersive X-ray spectroscopy and atomic force microscopy, chemical analysis and magnetic measurements. Graphical Abstract: [Figure not available: see fulltext.] © 2016, Springer Science+Business Media Dordrecht.


Svediene J.,Laboratory of Biodeterioration Research | Raudoniene V.,Laboratory of Biodeterioration Research | Loziene K.,Nature Research Center | Bridziuviene D.,Laboratory of Biodeterioration Research | And 2 more authors.
Journal of Essential Oil-Bearing Plants | Year: 2015

In this study, we determined the antimicrobial activity of Thymus pulegioides L. chemotypes geraniol/geranial/neral (G/G/N), carvacrol/γ-terpinene/p-cymeme (C/γT/pC) and linalool (L) essential oils. We observed the effect of pH and of the essential oils on microorganisms. Activities of essential oils of different Thymus pulegioides chemotypes were tested with three pH’s, i.e. 3, 6, and 9. The essential oils from T. pulegioides chemotype G/G/N were the most active, L – least active. The results of the bioassays showed a significant inhibition of fungal spore germination by different concentration of G/G/N chemotype. The studied chemotypes were more active than the synthetic preservative (potassium sorbate and sodium benzoate) which displayed an antifungal activity at pH 3 and 6. The statistically significant effect of pH increases with decreasing of essential oil concentration of C/γT/pC chemotype, while the statistically significant effect of pH decreases with decreasing of essential oil concentration of L chemotype. © 2015, Har Krishan Bhalla & Sons.


Novickij V.,High Magnetic Field Institute | Grainys A.,High Magnetic Field Institute | Lastauskiene E.,VU | Kananaviciute R.,VU | And 6 more authors.
BioMed Research International | Year: 2015

Candida lusitaniae is an opportunistic yeast pathogen, which can readily develop resistance to antifungal compounds and result in a complex long-term treatment. The efficient treatment is difficult since structure and metabolic properties of the fungal cells are similar to those of eukaryotic host. One of the potential methods to improve the inhibition rate or the cell permeability to inhibitors is the application of electroporation. In this work we investigated the dynamics of the growth inhibition and membrane permeabilization of C. lusitaniae by utilizing the various pulse shape and duration electric field pulses. Our results indicated that single electroporation procedure using 8 kV/cm electric field may result in up to 51±5% inhibition rate. Also it has been experimentally shown that the electroporation pulse shape may influence the inhibitory effect; however, the amplitude of the electric field and the pulse energy remain the most important parameters for definition of the treatment outcome. The dynamics of the cell membrane permeabilization in the 2-8 kV/cm electric field were overviewed. © 2015 V. Novickij et al.


PubMed | High Magnetic Field Institute, State Research Institute Center for Innovative Medicine, VU and Laboratory of Biodeterioration Research
Type: | Journal: BioMed research international | Year: 2015

Candida lusitaniae is an opportunistic yeast pathogen, which can readily develop resistance to antifungal compounds and result in a complex long-term treatment. The efficient treatment is difficult since structure and metabolic properties of the fungal cells are similar to those of eukaryotic host. One of the potential methods to improve the inhibition rate or the cell permeability to inhibitors is the application of electroporation. In this work we investigated the dynamics of the growth inhibition and membrane permeabilization of C. lusitaniae by utilizing the various pulse shape and duration electric field pulses. Our results indicated that single electroporation procedure using 8kV/cm electric field may result in up to 51 5% inhibition rate. Also it has been experimentally shown that the electroporation pulse shape may influence the inhibitory effect; however, the amplitude of the electric field and the pulse energy remain the most important parameters for definition of the treatment outcome. The dynamics of the cell membrane permeabilization in the 2-8kV/cm electric field were overviewed.

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