BioClinicum Center

Moscow, Russia

BioClinicum Center

Moscow, Russia
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Zakharyants A.A.,BioClinicum Center | Burmistrova O.A.,BioClinicum Center | Poloznikov A.A.,BioClinicum Center
Bulletin of Experimental Biology and Medicine | Year: 2017

The possibility of interactions between warfarin and dasatinib and their interactions with other drugs metabolized by cytochrome P450 isoform CYP3A4 was demonstrated using a previously created cytochrome P450 substrate—inhibitor panel for preclinical in vitro studies of drug biotransformation on a 3D histotypical microfluidic cell model of human liver (liver-on-a-chip technology). Dasatinib and warfarin are inhibitors of CYP2C19 isoform and hence, can interfere the drugs metabolized by this isoform. Our findings are in line with the data obtained on primary culture of human hepatocytes and suggest that the model can be used in preclinical in vitro studies of drugs. © 2017, Springer Science+Business Media New York.


Senyavina N.V.,BioClinicum Center | Khaustova S.A.,BioClinicum Center
Bulletin of Experimental Biology and Medicine | Year: 2013

Metabolome analysis of the serum from pregnant patients aimed at detection of low-molecular-weight biomarkers of gestation process disorders indicated a relationship between themetabolic profile of maternal serum and risk of gestosis. In women with pre-eclampsia or preterm delivery, analysis of serum purine metabolites revealed changes in the metabolite concentrations, associated with pregnancy complications. © 2013 Springer Science+Business Media New York.


Maltseva D.V.,BioClinicum Center | Ryabenko E.A.,BioClinicum Center | Yashin D.V.,Russian Academy of Sciences | Khaustova S.A.,BioClinicum Center | Shkurnikov M.Yu.,Russian Academy of Medical Sciences
Bulletin of Experimental Biology and Medicine | Year: 2012

The effects of 30-min medium-intensity exercise on the expression of genes encoding heat shock protein 70 (HSPA1A) and its cochaperones HSP-70-binding protein 1 (HSPBP1) and Tag7 (PGLYRP1) in human leukocytes were studied. Transcription activities of HSPA1A and PGLYRP1 genes increased immediately after medium-intensity exercise, while activity of HSPBP1 gene remained unchanged. During recovery after exercise, the expression of HSPA1A gene virtually did not change, while the expression of PGLYRP1 gene continued to increase and after 90 min more than 2-fold surpassed the basal level. © 2012 Springer Science+Business Media New York.


Fomicheva K.A.,National Health Research Institute | Osip'yants A.I.,National Health Research Institute | Knyazev E.N.,National Health Research Institute | Samatov T.R.,BioClinicum Center | Shkurnikov M.Y.,National Health Research Institute
Bulletin of Experimental Biology and Medicine | Year: 2017

We studied the profile of miRNA secreted into culture medium by DU145 prostate cancer cells and identified a subset of miRNAs characterized by the absence of correlation of their content in the cell and medium, which is likely a result of specific secretion. Three of these miRNA, hsa-miR-4417, hsa-miR-3175, and hsa-miR-6782-5p, exhibit the highest expression and are candidate circulating biomarkers for metastatic activity of prostate cancer. Two of these miRNA are coded by introns of genes linked with genome stability maintenance and chromatin remodeling regulation. © 2017 Springer Science+Business Media New York


Maltseva D.V.,BioClinicum Center
Bulletin of experimental biology and medicine | Year: 2012

The effects of 30-min medium-intensity exercise on the expression of genes encoding heat shock protein 70 (HSPA1A) and its cochaperones HSP-70-binding protein 1 (HSPBP1) and Tag7 (PGLYRP1) in human leukocytes were studied. Transcription activities of HSPA1A and PGLYRP1 genes increased immediately after medium-intensity exercise, while activity of HSPBP1 gene remained unchanged. During recovery after exercise, the expression of HSPA1A gene virtually did not change, while the expression of PGLYRP1 gene continued to increase and after 90 min more than 2-fold surpassed the basal level.


Khaustova N.A.,BioClinicum Center | Makeeva D.S.,Moscow State University | Kondrashina O.V.,Moscow State University | Fedotov N.N.,Moscow State University | And 6 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2014

We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76∈±∈0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies. © 2014 Springer Science+Business Media New York.


PubMed | BioClinicum Center
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2012

The effects of 30-min medium-intensity exercise on the expression of genes encoding heat shock protein 70 (HSPA1A) and its cochaperones HSP-70-binding protein 1 (HSPBP1) and Tag7 (PGLYRP1) in human leukocytes were studied. Transcription activities of HSPA1A and PGLYRP1 genes increased immediately after medium-intensity exercise, while activity of HSPBP1 gene remained unchanged. During recovery after exercise, the expression of HSPA1A gene virtually did not change, while the expression of PGLYRP1 gene continued to increase and after 90 min more than 2-fold surpassed the basal level.


PubMed | BioClinicum Center
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2013

Metabolome analysis of the serum from pregnant patients aimed at detection of low-molecular-weight biomarkers of gestation process disorders indicated a relationship between the metabolic profile of maternal serum and risk of gestosis. In women with pre-eclampsia or preterm delivery, analysis of serum purine metabolites revealed changes in the metabolite concentrations, associated with pregnancy complications.


PubMed | BioClinicum Center
Type: Comparative Study | Journal: Bulletin of experimental biology and medicine | Year: 2014

We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.

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