Biochemical Recovery Group

Birmingham, United Kingdom

Biochemical Recovery Group

Birmingham, United Kingdom
SEARCH FILTERS
Time filter
Source Type

Luechau F.,Biochemical Recovery Group | Ling T.C.,Biochemical Recovery Group | Lyddiatt A.,Biochemical Recovery Group | Lyddiatt A.,Northumbria University
Biochemical Engineering Journal | Year: 2010

The main parameters which influence the behaviour of phase separation in a single-stage Kühni-type aqueous two-phase extraction column containing polyethylene (PEG) and di-potassium hydrogen phosphate were characterised. Two aqueous two-phase system (ATPS) composed of 12% (w/w) PEG 1450 and 12% (w/w) di-potassium hydrogen phosphate (designated as 12/12) and 12% (w/w) PEG 1450 and 11% (w/w) di-potassium hydrogen phosphate (designated as 12/11) were chosen in this study. The hold-up εD increased with increasing impeller speeds and mobile phase flow rates. Phase separation for the 12/11 system was slower than that for the 12/12 system, which resulted in higher dispersed phase hold-up values for the 12/11 system. For 12/12 system, mass transfer of plasmid DNA (pDNA) from the dispersed mobile phase to the stationary phase increased rapidly with increasing impeller speeds of 130, 160 and 200 rpm which was reflected in the decreased values for CT/CTo. The degree of back-mixing quantified by the axial dispersion coefficient Dax was estimated to be 2.7 × 10-6 m2 s-1. © 2010 Elsevier B.V. All rights reserved.


Luechau F.,Biochemical Recovery Group | Ling T.C.,Biochemical Recovery Group | Lyddiatt A.,Biochemical Recovery Group | Lyddiatt A.,Northumbria University
Biochemical Engineering Journal | Year: 2010

In this paper, we have developed a descriptive model and methods for the up-scaled interfacial partition of bioparticles in polymer-salt aqueous two-phase systems (ATPS). The model is only valid for ideal spherical particles. The model might shed some light on the mechanism of interfacial partition, which allows one to draft some process routes for its potential operation on a larger scale. © 2010 Elsevier B.V. All rights reserved.


Selvakumar P.,Biochemical Recovery Group | Ling T.C.,Biochemical Recovery Group | Ling T.C.,University Putra Malaysia | Walker S.,Biochemical Recovery Group | And 2 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2010

Aqueous two-phase systems (ATPS) have been widely adopted for the combined purpose of solid liquid separation, and recovery and purification of bioproducts such as proteins, viruses and organelles from biological feedstocks and fermentation broth. However, in spite of potential advantages over other techniques applied to concentrated biological feedstocks, ATPS have been applied at process scale only by a few industries and research establishments. ATPS are sensitive to loading with modest to extreme quantities of biological feedstock due to the contribution of that material to phase formation in combination with the conventional phase-forming chemicals. This causes problem associated with the definition and manipulation of loaded working systems, which may be addresses as in the present study with the aid of distribution analysis of radiolabel led analytes (DARA) in representative process samples. The present study focussed on establishing a generic description for characterising ATPS loaded with biological feedstocks and the redefinition of the biological feedstock loaded system composition in terms of phase forming chemical equivalents. This evaluation will be useful to achieve ATPS process implementation where phase recycle/reuse is adopted without compromise to process operations and consistent protein recovery performance. © 2010 Elsevier B.V. All rights reserved.

Loading Biochemical Recovery Group collaborators
Loading Biochemical Recovery Group collaborators