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Skopje, Macedonia

Davalieva K.,Macedonian Academy of science and Arts | Kiprijanovska S.,Macedonian Academy of science and Arts | Komina S.,University of Macedonia | Petrusevska G.,University of Macedonia | And 2 more authors.
Proteome Science | Year: 2015

Despite the overall success of prostate specific antigen (PSA) in screening and detection of prostate cancer (PCa), its use has been limited due to the lack of specificity. The principal driving goal currently within PCa research is to identify non-invasive biomarker(s) for early detection of aggressive tumors with greater sensitivity and specificity than PSA. In this study, we focused on identification of non-invasive biomarkers in urine with higher specificity than PSA. We tested urine samples from PCa and benign prostatic hyperplasia (BPH) patients by 2-D DIGE coupled with MS and bioinformatics analysis. Statistically significant (p < 0.05), 1.8 fold variation or more in abundance, showed 41 spots, corresponding to 23 proteins. The Ingenuity Pathway Analysis showed significant association with the Acute Phase Response Signaling pathway. Nine proteins with differential abundances were included in this pathway: AMBP, APOA1, FGA, FGG, HP, ITIH4, SERPINA1, TF and TTR. The expression pattern of 4 acute phase response proteins differed from the defined expression in the canonical pathway. The urine levels of TF, AMPB and HP were measured by immunoturbidimetry in an independent validation set. The concentration of AMPB in urine was significantly higher in PCa while levels of TF and HP were opposite (p < 0.05). The AUC for the individual proteins ranged from 0.723 to 0.754. The combination of HP and AMBP yielded the highest accuracy (AUC = 0.848), greater than PSA. The proposed biomarker set is quickly quantifiable and economical with potential to improve the sensitivity and specificity ofPCa detection. © 2015 Davalieva et al.

Sifianou P.,General and Maternity Hospital | Zisis D.,Biochemical laboratory
Growth Hormone and IGF Research | Year: 2012

Objective: The aim of this study was to investigate whether readily available laboratory tests may aid in the identification of growth-restricted neonates. Design: Cord serum levels of 15 chemical analytes, including insulin-like growth factor I (IGF-I) and insulin-like growth factor binding protein 3 (IGFBP-3) were measured in newborns ≥36weeks gestational age (GA). Based on the number of anthropometric indices (out of four) with values ≤25th centile for GA, the babies were allocated into three groups, i.e., Group250, Group251 and Group252 corresponding to neonates with 0, 1 and 2 or more indices, respectively, that were ≤25th centile for GA. Furthermore, two composite variables were developed: A25 (Group250 and Group251) and B25 (Group250 and Group252). The data were evaluated by the Mann-Whitney test and multiple regression analyses. Results: Cord serum triglycerides and total cholesterol levels were significantly higher in Group252 compared to Group250 (p values 0.004 and 0.0009, respectively). The triglycerides almost doubled the power of the variable B25 for predicting IGF-I levels and were found to have a highly significant, negative association with the IGF-I levels (p<0.0001). The IGF-I along with the IGFBP-3 levels explained almost one third of the variation of triglycerides. Conclusion: Cord serum triglycerides can assist in the identification of growth-restricted neonates. The novel finding of the association of triglycerides with IGF-I calls for further research as this can illuminate unknown aspects of the fetal lipid metabolism. © 2012 Elsevier Ltd.

Ballester M.,Hospital Clinico Universitario | Llorens J.,Hospital Clinico Universitario | Garcia-De-La-Asuncion J.,Hospital Clinico Universitario | Perez-Griera J.,Biochemical laboratory | And 2 more authors.
European Journal of Anaesthesiology | Year: 2011

Context Myocardial oxidative stress plays an essential role in the pathogenesis of ischaemia-reperfusion injury associated with coronary artery bypass grafting (CABG). Both propofol and volatile anaesthetics have been shown to reduce reactive oxygen species in experimental and clinical studies. Main objective To compare the influence of sevoflurane and propofol on myocardial oxidative stress markers (F2-isoprostanes and nitrates/nitrites) in coronary sinus blood samples from patients undergoing off-pump CABG. Design and setting Randomised controlled clinical study of patients scheduled for off-pump CABG in a tertiary academic university hospital from June 2007 to August 2009. Forty patients consented to enrolment and were assigned to receive either propofol or sevoflurane. Interventions Upon completion of the proximal anastomosis, a retroplegia cannula was inserted in the coronary sinus to obtain blood samples, according to the study protocol. Main outcome measures Markers of lipoperoxidation (F2-isoprostanes) and nitrosative stress (nitrates/nitrites) were measured in coronary sinus blood samples at three time points: after the end of the proximal anastomosis (T1), after completion of all grafts (T2) and 15min after revascularisation (T3). Results Of the 40 recruited patients, 38 fully completed the study. In the sevoflurane group (n - 20), concentrations of oxidative stress markers in the coronary sinus remained almost constant and were significantly lower than those in the propofol group (n=18) at all time points. F2-isoprostanes concentrations were as follows at T1:sevoflurane group 37.2 ±27.5 pgml 1 vs. propofol group 170.7±30.9pgml -1 [95% confidence interval (CI) 112.16-155.08, P<0.0001); at T2:sevoflurane group 31.94±24.6pgml -1 vs. propofol group 171.6±29.7 pgm -1 (95% CI 119.78-159.63, P< 0.0001); and at T3:sevoflurane group 23.8 ±13.0pgml -1 vs. propofol group 43.6±31 pgml -1 (95% CI 2.87-36.63, P = 0.023). Conclusion In patients undergoing off-pump CABG, sevoflurane showed better antioxidative properties than propofol. © 2011 Copyright European Society of Anaesthesiology.

Hardavella G.F.,National and Kapodistrian University of Athens | Dionellis G.S.,National and Kapodistrian University of Athens | Kantza C.G.,Biochemical laboratory | Koulouris N.G.,National and Kapodistrian University of Athens | Alchanatis M.,National and Kapodistrian University of Athens
Recent Patents on Cardiovascular Drug Discovery | Year: 2011

Recent advances in technology and novel pharmaceutical research findings have added new grounds in the fields of medical treatment and quality of life of patients diagnosed with pulmonary arterial hypertension (PAH). Collective assessment of new data is mandatory and useful for specialist medical doctors. This review aims to present the latest therapeutic developments of the last two years (2009-2010) in PAH. Moreover, recent patents (of the year 2010) regarding therapeutic novelties in PAH that expand treatment modalities, are hereby presented. © 2011 Bentham Science Publishers Ltd.

Pizza V.,Neurophysiopathology Service | Agresta A.,Neurophysiopathology Service | Lamaida E.,Neurophysiopathology Service | Lamaida N.,Neurophysiopathology Service | And 2 more authors.
Open Neurology Journal | Year: 2012

The relationship between genetic polymorphisms and migraine as a cause of an increased risk of thrombotic disorders development is still debated In this respect, factor V Leiden, factor V (H1299R), prothrombin G20210A, factor XIII (V34L), β-fibrinogen, MTHFR (C677T), MTHFR (A1298C), APO E, PAI-1, HPA-1 and ACE I/D seem to play a determinant role in vascular diseases related to migraine. The present review analyzes both the incidence of the above genetic vascular mutations in migraineurs and the most recent developments related to genetic polymorphisms and migraine. © Finsterer and Stöllberger.

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