Sedrati K.,University of Mentouri Constantine |
Abadi N.,University of Mentouri Constantine |
Abadi N.,Biochemical Laboratory |
Sifi K.,University of Mentouri Constantine |
And 3 more authors.
International Journal of Pharmaceutical Sciences Review and Research | Year: 2016
Two common single-nucleotide polymorphisms (SNPs) of Methylenetetrahydrofolate reductase (MTHFR) gene, C677T (rs1801133) and A1298C (rs1801131), reduce enzyme activity. These SNPs seems to influence the effectiveness of treatment with fluoropyrimidine in breast cancer chemotherapy, but further studies have yielded contradictory results. We tested whether these two polymorphisms are determinants of clinical outcome. 117 breast cancer patients receiving 5-FU based chemotherapy was genotyped for the tow variants in MTHFR gene using PCR-RFLP, relationships between genetic variants and tumor response were assessed. The response rate (48.7 % responders, 51.3 % non-responders) was related to 5-FU pharmacogenetic. MTHFR genotypes for C677T were associated to response (p=0.01, χ2 = 8.18). However, no association was observed for the A1298C polymorphism (p=0.79, χ2 = 0.46). Our data provide that C677T MTHFR polymorphism affect the clinical outcome for fluoropyrimidine chemotherapy. The MTHFR polymorphism may be a useful pharmacogenetic determinant for predict of benefit from 5-FU based chemotherapy. © 2016, Global Research Online. All rights reserved.
Ballester M.,Hospital Clinico Universitario |
Llorens J.,Hospital Clinico Universitario |
Garcia-De-La-Asuncion J.,Hospital Clinico Universitario |
Perez-Griera J.,Biochemical Laboratory |
And 3 more authors.
European Journal of Anaesthesiology | Year: 2011
Context Myocardial oxidative stress plays an essential role in the pathogenesis of ischaemia-reperfusion injury associated with coronary artery bypass grafting (CABG). Both propofol and volatile anaesthetics have been shown to reduce reactive oxygen species in experimental and clinical studies. Main objective To compare the influence of sevoflurane and propofol on myocardial oxidative stress markers (F2-isoprostanes and nitrates/nitrites) in coronary sinus blood samples from patients undergoing off-pump CABG. Design and setting Randomised controlled clinical study of patients scheduled for off-pump CABG in a tertiary academic university hospital from June 2007 to August 2009. Forty patients consented to enrolment and were assigned to receive either propofol or sevoflurane. Interventions Upon completion of the proximal anastomosis, a retroplegia cannula was inserted in the coronary sinus to obtain blood samples, according to the study protocol. Main outcome measures Markers of lipoperoxidation (F2-isoprostanes) and nitrosative stress (nitrates/nitrites) were measured in coronary sinus blood samples at three time points: after the end of the proximal anastomosis (T1), after completion of all grafts (T2) and 15min after revascularisation (T3). Results Of the 40 recruited patients, 38 fully completed the study. In the sevoflurane group (n - 20), concentrations of oxidative stress markers in the coronary sinus remained almost constant and were significantly lower than those in the propofol group (n=18) at all time points. F2-isoprostanes concentrations were as follows at T1:sevoflurane group 37.2 ±27.5 pgml 1 vs. propofol group 170.7±30.9pgml -1 [95% confidence interval (CI) 112.16-155.08, P<0.0001); at T2:sevoflurane group 31.94±24.6pgml -1 vs. propofol group 171.6±29.7 pgm -1 (95% CI 119.78-159.63, P< 0.0001); and at T3:sevoflurane group 23.8 ±13.0pgml -1 vs. propofol group 43.6±31 pgml -1 (95% CI 2.87-36.63, P = 0.023). Conclusion In patients undergoing off-pump CABG, sevoflurane showed better antioxidative properties than propofol. © 2011 Copyright European Society of Anaesthesiology.
Diareme M.,Biochemical Laboratory |
Markou A.,General Hospital of Zakynthos |
Karkalousos P.,Technological Educational Institute of Athens |
Acta Microbiologica Hellenica | Year: 2014
Prostatic antigen (PSA) or P-30 antigen is produced by the glandular epithelium of prostate. It circulates in blood in very low levels but in pathological situations, such as cancer, increases dramatically. In current study General Hospital of Zakynthos and EOPPY of Zakynthos tried to estimate PSA reference values and to confirm common knowledge about its diagnostic value. For that reason, 1320 PSA values and 170 Free/Total PSA values (F/T ratio) were collected. All determinations followed by the patients' diagnosis and divided in three groups: normal, hypertrophy and prostate cancer. The data were analyzed by non-parametric statistical tests in order to investigate any difference between the men's groups. We make also ROC curves for the estimation of reference values. According to our results, it was proved that increase of age increases also the variance of PSA values. Significant statistical difference observed after 80 years old. Between different groups of patients there were significant differences. In normal men PSA values varied between 1,3 and 1,8 ng/ml and in men with prostate hypertrophy PSA values varied between 6,6 and 7,6 ng/ml and in men with prostate cancer between 34,9 and 63,2 ng/ml. ROC curves confirmed the higher diagnostic capability of F/T ratio against PSA in the diagnosis of prostate cancer. The upper value of PSA dispersion is increasing with the age but the lower value remains constant. Prostate cancer causes a large dispersion of PSA values. Our sample's values didn't prove the ability of PSA and F/T ratio to diagnose prostate hypertrophy and cancer.
Kasmi G.,University of Tirana |
Refatllari E.,Biochemical Laboratory |
Dumani S.,Service of Heart Surgery |
Refatllari A.,Service of Heart Surgery
Clinical Laboratory | Year: 2014
Staphylococcus aureus is now the most common cause of infective endocarditis (IE) in many areas of the developed world. Patients with S. aureus IE exhibit different characteristics compared to patients with IE deriving from other organisms . IE in general is a complication of bacteremia following invasive procedures .
PubMed | Biochemical Laboratory
Type: Journal Article | Journal: Biocontrol science | Year: 2013
Some studies show the uptake of disinfectants on medical devices but no studies on their cytotoxicity have been reported. This study aimed to assess that cytotoxicity in a 3-dimensional culture system using HeLa cells grown in matrices composed of collagen. Plastic materials were soaked in the use solutions of the widely used high-level disinfectants, glutaraldehyde (GA), ortho-phthalaldehyde (OPA) and peracetic acid (PAA). After being rinsed, they were allowed to dry and were embedded into the cell medium to investigate the cytotoxicity of the residual disinfectants. Cytotoxicity was observed with the polyvinyl chloride, polyurethane and silicon tubes soaked in GA and OPA, indicating that both disinfectants were absorbed in the test pieces, whereas for PAA, none was observed. As for the polytetrafluoroethylene (PTFE) tubes, no disinfectant displayed cytotoxicity. GA and OPA are primary irritants, having a potential to cause anaphylaxis and other forms of allergic reactions. There should be consideration not only about the toxicity of the residual disinfectant from poor rinsing, but also about the toxicity that would result from the disinfectants that were absorbed and consequently released from the medical devices or materials.
PubMed | Biochemical Laboratory
Type: Comparative Study | Journal: Biocontrol science | Year: 2013
Most critical instruments are not designed for heat sterilization and autoclaving. These items are usually treated with chemical agents such as peracetic acid(PAA), glutaraldehyde (GA) and ortho-phthalaldehyde (OPA). MTT assay is often used to evaluate the in vitro cytotoxicity of these chemical agents. In this study, disinfectants were allowed to come in direct contact with cells. Their cytotoxicity was evaluated based on cell viability and adhesive properties. The results obtained from the direct contact method were compared with those obtained from the conventional MTT assay wherein the disinfectants were added into a nutrient medium. It was found that the two methods yielded very different results, especially when aldehyde- and halogen-containing disinfectants were tested, and that toxicity may be underestimated in the MTT assay. Hence, it can be assumed that the direct contact assay is more accurate when evaluating the cytotoxicity of residual chemicals. It was also observed that the cytotoxicity of PAA was lower than that of GA and OPA.
PubMed | Biochemical Laboratory
Type: Journal Article | Journal: The Journal of general physiology | Year: 2010
This paper deals with the relation between substrate concentration and velocity in the case of the reduction of methylene blue and of the other oxidation-reduction indicators of Clark by B. coli in the presence of succinic acid and glucose. This system is compared with starch and barley amylase. Reasons are given for considering the mechanism as an adsorption phenomenon.